CAJ | 학술논문

目的：探讨多西环素对肾间质纤维化的影响及可能机制。方法采用单侧输尿管结扎术( UUO )致肾小管间质纤维化大鼠模型。将72只SD大鼠随机分为假手术组、模型组、多西环素治疗组[8 mg/( kg·d )]。手术后7，14，21 d时分别处死各组中6只大鼠，用免疫组化方法观察大鼠肾组织中转化生长因子-β1( TGF-β1)、基质金属蛋白酶2( MMP-2)表达情况。行HE及Masson染色评定大鼠肾脏的病理情况。结果模型组各时间点MMP-2表达明显低于同期假手术组( P<0.05)，多西环素组各时间点MMP-2表达明显低于同期模型组( P<0.05)，多西环素组术后14 d及术后21 d低于术后7 d。模型组各时间点TGF-β1表达明显高于同期假手术组( P<0.05)，而多西环素组术后7 d TGF-β1表达明显低于同期模型组，多西环素组术后14 d及术后21 d高于术后7 d。结论多西环素在肾小管间质纤维化的早期对肾脏有保护作用。
목적：탐토다서배소대신간질섬유화적영향급가능궤제。방법채용단측수뇨관결찰술( UUO )치신소관간질섬유화대서모형。장72지SD대서수궤분위가수술조、모형조、다서배소치료조[8 mg/( kg·d )]。수술후7，14，21 d시분별처사각조중6지대서，용면역조화방법관찰대서신조직중전화생장인자-β1( TGF-β1)、기질금속단백매2( MMP-2)표체정황。행HE급Masson염색평정대서신장적병리정황。결과모형조각시간점MMP-2표체명현저우동기가수술조( P<0.05)，다서배소조각시간점MMP-2표체명현저우동기모형조( P<0.05)，다서배소조술후14 d급술후21 d저우술후7 d。모형조각시간점TGF-β1표체명현고우동기가수술조( P<0.05)，이다서배소조술후7 d TGF-β1표체명현저우동기모형조，다서배소조술후14 d급술후21 d고우술후7 d。결론다서배소재신소관간질섬유화적조기대신장유보호작용。
Objective To investigate the effect of doxycycline on the renal interstitial fibrosis in rats and its possible mechanism. Methods The unilateral ureteral obstruction ( UUO ) was adopted to create the renal tubular interstitial fibrosis model in rats. 72 normal female Sprague-Dawley rats were randomly divided into the sham-operation group, UUO model group and doxycycline treatment [ 8 mg/ ( kg·d ) ] group. 6 rats in each group were respectively sacrificed on 7, 14, 21 d after surgery. Immunohistochemistry was adopted to detect the expression of transforming growth factor-β1 ( TGF-β1 ) and matrix metalloproteinase-2 ( MMP-2 ) in the obstructed renal tissue. The HE staining and the Masson staining were performed to evaluate the renal pathological status in each group. Results The expression of MMP-2 at different time points in the UUO model group was significantly lower than that in the sham-operation group ( P<0. 05 ) , and the expression of MMP-2 at different time points in the doxycycline group was significantly lower than that in the UUO model group ( P<0. 05 ) , the expression of MMP-2 on postoperative 14, 21 d in the doxycycline group were lower than that on postoperative 7 d. The expression of TGF-β1 at different time points in the UUO model group was significantly higher than that in the sham-operated group ( P<0. 05 ) , but the expression of TGF-β1 on postoperative 7 d in the doxycycline group was significantly lower than that in the UUO model group, which on postoperative 14, 21 d in the doxycycline group was significantly higher than that an post-operative 7 d. Conclusion Doxycycline has a protective effect on the early tubulointerstitial fibrosis.