CAJ | 학술논문

目的：研究伊伐布雷定（ivabradine，Iva）对糖尿病（DM）大鼠心肌的保护作用及机制。方法建立链脲佐菌素（STZ）诱导Ⅰ型糖尿病大鼠模型，将54只实验大鼠随机分为对照（ NC）组、DM组、Iva低、中、高剂量组、阳性对照药（卡维地洛）组。测血糖、体重、心脏重量和血清中乳酸脱氢酶（LDH）、肌酸激酶（CK），同时测定心肌组织超氧化物歧化酶（SOD）活性和丙二醛（ MDA）含量，观察心肌结缔组织生长因子（ CTGF）表达的变化。结果与NC组相比，DM组大鼠血清中LDH、CK活性显著升高（P＜0．05），心肌组织SOD活性显著降低（P＜0．05），经Iva治疗后LDH、CK活性显著降低，SOD活性显著升高（P＜0．05）；DM组心肌中MDA含量与NC组相比显著升高，Iva组MDA含量升高显著降低（P＜0．05）。与NC组相比，DM组大鼠心肌中CTGF表达显著升高（P＜0．05），应用Iva干预治疗后，CTGF表达的升高显著被抑制（P＜0．05）。结论 Iva可显著升高糖尿病大鼠心肌组织SOD活性，抑制CTGF表达升高和脂质过氧化，从而对糖尿病大鼠心肌损伤产生保护作用。
목적：연구이벌포뢰정（ivabradine，Iva）대당뇨병（DM）대서심기적보호작용급궤제。방법건립련뇨좌균소（STZ）유도Ⅰ형당뇨병대서모형，장54지실험대서수궤분위대조（ NC）조、DM조、Iva저、중、고제량조、양성대조약（잡유지락）조。측혈당、체중、심장중량화혈청중유산탈경매（LDH）、기산격매（CK），동시측정심기조직초양화물기화매（SOD）활성화병이철（ MDA）함량，관찰심기결체조직생장인자（ CTGF）표체적변화。결과여NC조상비，DM조대서혈청중LDH、CK활성현저승고（P＜0．05），심기조직SOD활성현저강저（P＜0．05），경Iva치료후LDH、CK활성현저강저，SOD활성현저승고（P＜0．05）；DM조심기중MDA함량여NC조상비현저승고，Iva조MDA함량승고현저강저（P＜0．05）。여NC조상비，DM조대서심기중CTGF표체현저승고（P＜0．05），응용Iva간예치료후，CTGF표체적승고현저피억제（P＜0．05）。결론 Iva가현저승고당뇨병대서심기조직SOD활성，억제CTGF표체승고화지질과양화，종이대당뇨병대서심기손상산생보호작용。
Objective To investigate the effect of Ivabradine ( Iva) on the myocardial injury and the potential mechanisms of diabetic rats.Methods Diabetic rats were induced by intraperitoneal injection of streptozotocin .The alterations of weight,blood glucose,and heart weight were assessed respectively .The serum lactate dehydrogenase (LDH),creatine kinase(CK) activities,and superoxide dis-mutase(SOD) activities as well as malondialdehyde (MDA) content in myocardium were also determined .Furthermore,the expressions of CTGF in myocardium were examined by western blot .Results In DM group,body weight of rats was significantly decreased ,while blood glucose was not significantly higher as compared with control group (P<0.05).The activities of LDH and CK in serum were sig-nificantly higher than those of NC group ,which could be significantly suppressed by Iva treatment (P<0.05).However,SOD in myo-cardium of DM group was significantly lower than that of control group (P<0.05),which could be suppressed by Iva treatment (P<0.05).Moreover,the MDA level and CTGF expression in myocardium of DM group were markedly upregulated as compared with DM group,while these alterations were significantly attenuated by Iva (P<0.05).Conclusions Iva has a protective effect on myocardial injury in diabetic rats ,which might be associated with suppressing the alterations of SOD activities and the increase of CTGF expression in myocardium .