CAJ | 학술논문

目的建立同时测定一清软胶囊中8种有效成分含量的方法.方法采用Xtimate-C 18色谱柱,0.2％磷酸水(A)和乙腈(B)梯度洗脱,流速0.6 ml/min,检测波长280 nm,柱温30℃.结果盐酸小檗碱、盐酸黄连碱、黄芩素、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在进样量范围内与峰面积线性关系良好,r＞ 0.9997,仪器精密度和稳定性RSD均＜2％,各成分的回收率在98％～103％ (n=3).测定了3批一清软胶囊中8种指标成分的含量.结论建立的方法可同时测定一清软胶囊中8种有效成分的含量,方法简单、重复性好.
목적 건립동시측정일청연효낭중8충유효성분함량적방법.방법 채용Xtimate-C 18색보주,0.2％린산수(A)화을정(B)제도세탈,류속0.6 ml/min,검측파장280 nm,주온30℃.결과 염산소벽감、염산황련감、황금소、호회대황소、대황산、대황소、대황분화대황소갑미분별재진양량범위내여봉면적선성관계량호,r＞ 0.9997,의기정밀도화은정성RSD균＜2％,각성분적회수솔재98％～103％ (n=3).측정료3비일청연효낭중8충지표성분적함량.결론 건립적방법가동시측정일청연효낭중8충유효성분적함량,방법간단、중복성호.
Objective To establish a method for simultaneous quantitative determination of eight active components in Yiqing Soft capsule.Methods The separation was performed on Xtimate-C 18 chromatographic column with mobile phase consisted of water (containing 0.2％ phosphoric acid)-acetonitrile (gradient elution) at a flow rate of 0.6 ml/min.Detecting wavelength was set at 280 nm and the column temperature was set at 30℃.Results The calibration curve of berberine,coptisine-hydrochloride,baicalein,aloe-emodin,rhein,emodin,chrysophanol and physcion showed good linear regression in test ranges (r ＞ 0.9995); and the overall recoveries were in the range of 97％-103％ (n=3).The overall RSD of precision test were less than 2％.Conclusion The extracting method is convenient and credible,and the quantitative method is accurate and repeatable,and it can be used in simultaneous quantitative determination of eight active components in Yiqing soft capsule.