CAJ | 학술논문

采用面心立方设计（CCF）研究了温度（16~32℃）和盐度（15~55）对亚心形四爿藻生长的联合效应。结果表明，温度与盐度的一次、二次效应及互作效应对亚心形四爿藻比生长速率影响都极显著（P <0.01），温度对亚心形四爿藻比生长速率的影响大于盐度。响应曲面法分析结果表明，当温度和盐度同时升高时，亚心形四爿藻比生长速率呈先上升后下降的趋势。构建亚心形四爿藻比生长速率与温度、盐度间的模型方程，模型的决定系数为0.9873，拟合度极高；预测决定系数为0.8824，表明可用于预测亚心形四爿藻比生长速率的变化。通过模型优化及验证实验，在温度、盐度组合为26℃/33时，亚心形四爿藻比生长速率达到最大值。
채용면심립방설계（CCF）연구료온도（16~32℃）화염도（15~55）대아심형사장조생장적연합효응。결과표명，온도여염도적일차、이차효응급호작효응대아심형사장조비생장속솔영향도겁현저（P <0.01），온도대아심형사장조비생장속솔적영향대우염도。향응곡면법분석결과표명，당온도화염도동시승고시，아심형사장조비생장속솔정선상승후하강적추세。구건아심형사장조비생장속솔여온도、염도간적모형방정，모형적결정계수위0.9873，의합도겁고；예측결정계수위0.8824，표명가용우예측아심형사장조비생장속솔적변화。통과모형우화급험증실험，재온도、염도조합위26℃/33시，아심형사장조비생장속솔체도최대치。
By using Central Composite Face-centered design (CCF), the joint effects of water temperature (16-32℃) and salinity (15-55) on the growth of Tetraselmis subcordiformis were examined in this study. The results showed that the linear, quadratic and the interactive effects of temperature and salinity on the specific growth rate (SGR) of Tetraselmis subcordiformis were highly significant (P<0.01). Through response surface methodology, it showed that the SGR varied with increasing water temperature and salinity in a curvilinear fashion and the effect of temperature was greater than that of salinity. A model equation about the relationships of the growth rate with the two factors was established, with the R2 as highly as 0.9873, suggesting that the overarching predictive capability of the model was satisfactory, and the Pred.R2 reached 0.8824, demonstrating that the model could be practicably applied to prediction. Through the optimization of the reliable model, the SGR reached its maximum (0.83) when the thermohaline combination was 26℃/33, with the desirability value 0.985. These experimental results could offer theoretical reference for the production of the Tetraselmis subcordiformis.