生物加工过程
生物加工過程
생물가공과정
CHINESE JOURNAL OF BIOPROCESS ENGINEERING
2014年
2期
70-76
,共7页
癌细胞%叶酸%亚酞菁
癌細胞%葉痠%亞酞菁
암세포%협산%아태정
cancer cells%folic acid%sub-phthalocyanine
以4氨基邻苯二腈、无水ZnCl2、叶酸等为原料,经固相合成法制备了三取代氨基亚酞菁锌( NH2 Sub Pc Zn),进一步通过酰胺反应合成了叶酸修饰的三取代氨基亚酞菁锌(FA Pc)。采用MTT方法评价了NH2 Sub Pc Zn、FA Pc以及四取代氨基酞菁锌( NH2 Pc Zn)对Hela细胞的毒性,毒性试验表明,加入2 mg/mL FA Pc,Hela细胞的抑制率超过60%;同时研究了FA Pc的活体成像特性,近红外荧光成像结果表明,FA Pc定向聚集在裸鼠的肿瘤肝肾部位且可以稳定12 h。因此说明FA Pc可用于活体肝癌的选择性成像。
以4氨基鄰苯二腈、無水ZnCl2、葉痠等為原料,經固相閤成法製備瞭三取代氨基亞酞菁鋅( NH2 Sub Pc Zn),進一步通過酰胺反應閤成瞭葉痠脩飾的三取代氨基亞酞菁鋅(FA Pc)。採用MTT方法評價瞭NH2 Sub Pc Zn、FA Pc以及四取代氨基酞菁鋅( NH2 Pc Zn)對Hela細胞的毒性,毒性試驗錶明,加入2 mg/mL FA Pc,Hela細胞的抑製率超過60%;同時研究瞭FA Pc的活體成像特性,近紅外熒光成像結果錶明,FA Pc定嚮聚集在裸鼠的腫瘤肝腎部位且可以穩定12 h。因此說明FA Pc可用于活體肝癌的選擇性成像。
이4안기린분이정、무수ZnCl2、협산등위원료,경고상합성법제비료삼취대안기아태정자( NH2 Sub Pc Zn),진일보통과선알반응합성료협산수식적삼취대안기아태정자(FA Pc)。채용MTT방법평개료NH2 Sub Pc Zn、FA Pc이급사취대안기태정자( NH2 Pc Zn)대Hela세포적독성,독성시험표명,가입2 mg/mL FA Pc,Hela세포적억제솔초과60%;동시연구료FA Pc적활체성상특성,근홍외형광성상결과표명,FA Pc정향취집재라서적종류간신부위차가이은정12 h。인차설명FA Pc가용우활체간암적선택성성상。
Zine amino-sub-phthalocyanine ( NH2-Snb-Pc-Zn) was prepared by solid-state reaction of 4-aminophthalonitrile,anhydrous zinc chloride, folic acid, amidation of the carboxyl.Folic acid ( FA ) modified Pc ( FA-Pc) complexes were obtained by MTT method to evaluate the toxicity to Hela cells with NH2-Sub-Pc-Zn,FA-Pc and four substituted amino-zinc-phthalocyanine(NH2-Pc-Zn).Toxicity test results showed that 2 mg/mL of FA-Pc led to the inhibition rate of Hela cells of more than 60%.The FA-Pc in vivo imaging characteristics were studied,near-infrared fluorescence imaging results showed that FA-Pc gathered directional in nude mice’ s tumor hepatorenal areas was stable for 12 h.FA-Pc could be used for whole-body fluorescence imaging.