CAJ | 학술논문

建立了快速、准确测定微生物油脂中花生四烯酸（ ARA）含量的气相色谱检测方法。选用DB 23毛细管色谱柱，设置合适的载气压力，采用FID检测器，对ARA含量进行了定量分析。测定结果表明：油脂中各脂肪酸组分可有效分离，分析时间仅需 min，ARA的回收率为.146％～100.634％，相对标准偏差为.175％。
건립료쾌속、준학측정미생물유지중화생사희산（ ARA）함량적기상색보검측방법。선용DB 23모세관색보주，설치합괄적재기압력，채용FID검측기，대ARA함량진행료정량분석。측정결과표명：유지중각지방산조분가유효분리，분석시간부수 min，ARA적회수솔위.146％～100.634％，상대표준편차위.175％。
Gas chromatography was performed for the determination of arachidonic acid ( ARA) produced by micro-organisms.Capillary column DB-23 was used with FID and suitable carrier gas for the detection.The result indicated that the method could separate fatty acids well in 20 min on good precision and recovery.The recovery rate of ARA was 90.146% to 100.634%,and the relative standard deviation was 4.175%.Thus,the method was effective for the detection of ARA produced by micro-organisms.