生物加工过程
生物加工過程
생물가공과정
CHINESE JOURNAL OF BIOPROCESS ENGINEERING
2014年
2期
51-55
,共5页
陆露%郑丽屏%赵培飞%王剑文
陸露%鄭麗屏%趙培飛%王劍文
륙로%정려병%조배비%왕검문
银%二氧化硅核壳型纳米粒%抑菌%香石竹镰刀菌%活性氧
銀%二氧化硅覈殼型納米粒%抑菌%香石竹鐮刀菌%活性氧
은%이양화규핵각형납미립%억균%향석죽렴도균%활성양
Ag-SiO2 core-shell nanoparticles%antifungus%Fusarium oxysporum%reactive oxygen species
利用抗坏血酸对AgNO3进行还原,生成银纳米粒核心,并通过正硅酸四乙酯的水解与聚合反应获得SiO2介孔外壳,制备平均粒径约为.9 nm的Ag SiO2核壳型纳米粒。 Ag SiO2纳米粒可以显著地抑制香石竹镰刀菌的生长,最小抑菌质量浓度为μg/mL,并可抑制香石竹镰刀菌菌丝生长和孢子分生。 Ag SiO2纳米粒处理~4 h后,菌丝体的过氧化氢酶、总超氧化物歧化酶、过氧化物酶活力增强,提示Ag SiO2纳米粒抗菌机制和活性氧诱导相关。
利用抗壞血痠對AgNO3進行還原,生成銀納米粒覈心,併通過正硅痠四乙酯的水解與聚閤反應穫得SiO2介孔外殼,製備平均粒徑約為.9 nm的Ag SiO2覈殼型納米粒。 Ag SiO2納米粒可以顯著地抑製香石竹鐮刀菌的生長,最小抑菌質量濃度為μg/mL,併可抑製香石竹鐮刀菌菌絲生長和孢子分生。 Ag SiO2納米粒處理~4 h後,菌絲體的過氧化氫酶、總超氧化物歧化酶、過氧化物酶活力增彊,提示Ag SiO2納米粒抗菌機製和活性氧誘導相關。
이용항배혈산대AgNO3진행환원,생성은납미립핵심,병통과정규산사을지적수해여취합반응획득SiO2개공외각,제비평균립경약위.9 nm적Ag SiO2핵각형납미립。 Ag SiO2납미립가이현저지억제향석죽렴도균적생장,최소억균질량농도위μg/mL,병가억제향석죽렴도균균사생장화포자분생。 Ag SiO2납미립처리~4 h후,균사체적과양화경매、총초양화물기화매、과양화물매활력증강,제시Ag SiO2납미립항균궤제화활성양유도상관。
The Ag-SiO2 core-shell nanoparticles were prepared.The mean size of the nanoparticles was 92.9 nm, and the nanoparticles demonstrated the activity of growth inhibition on Fusarium oxysporum f.sp.dianthi.The minimum inhibition concentration of the nanoparticles was 4 μg/mL.The nanoparticles could restrain the mycelia growth and conidial regeneration of the fungus.Moreover, the antioxidant enzyme activities of super oxygen dehydrogenases,catalase and peroxidase in mycelium were induced by the nanoparticle treatment for 2~4 h.The results suggested that the induced reactive oxygen species were also involved in the antimicrobial mechanism of the prepared nanoparticles.