CAJ | 학술논문

采用壳聚糖对合欢皮总多糖的絮凝纯化工艺进行研究。通过单因素和正交试验，得到优化的工艺条件：壳聚糖用量为生药量.8 mL/g，絮凝温度℃，絮凝时间 h，药液浓缩比（质量体积比）为∶10（g/mL），在此条件下，多糖保留率、脱蛋白率和多糖纯度分别为.23％、22.26％和.27％，优于传统的水提醇沉法。
채용각취당대합환피총다당적서응순화공예진행연구。통과단인소화정교시험，득도우화적공예조건：각취당용량위생약량.8 mL/g，서응온도℃，서응시간 h，약액농축비（질량체적비）위∶10（g/mL），재차조건하，다당보류솔、탈단백솔화다당순도분별위.23％、22.26％화.27％，우우전통적수제순침법。
Through single factor and orthogonal experimental designs, the optimun conditions were obtained as follows:the dosage of chitosan was 0.8 mL/g, temperature 35 ℃, flocculation time 4 h, determined as 1∶10 of drug density.Under the optimum conditions,polysaccharide retention rate,protein removal rate,and the purity of polysaccharide were obtained as follows:91.23%,22.26% and 47.27%, respectively.The flocculating purification of total polysaccharides was superior to the traditional method of extracting with water and depositing with alcohol.