CAJ | 학술논문

为了使谷氨酸棒杆菌较好地利用木糖生产有机酸，将来自Escherichia coli K 12的木糖异构酶基因xylA构建到表达载体pXMJ19中，导入Corynebacterium glutamicum ATCC13032Δldh中，成功表达了该酶基因。结果表明：重组菌株在以木糖为唯一C源进行发酵时，木糖的消耗速率为.54 g/（ L·h），木糖异构酶比酶活约为.54 U/mL；在以木糖和葡萄糖的混合糖为C源进行发酵时，菌株优先利用葡萄糖，在葡萄糖完全消耗后，菌株开始有效利用木糖；以木糖为唯一C源进行两阶段发酵时，琥珀酸的收率可达（0.62±0.003）g/g。
위료사곡안산봉간균교호지이용목당생산유궤산，장래자Escherichia coli K 12적목당이구매기인xylA구건도표체재체pXMJ19중，도입Corynebacterium glutamicum ATCC13032Δldh중，성공표체료해매기인。결과표명：중조균주재이목당위유일C원진행발효시，목당적소모속솔위.54 g/（ L·h），목당이구매비매활약위.54 U/mL；재이목당화포도당적혼합당위C원진행발효시，균주우선이용포도당，재포도당완전소모후，균주개시유효이용목당；이목당위유일C원진행량계단발효시，호박산적수솔가체（0.62±0.003）g/g。
In order to construct a strain of Corynebacterium glutamicum by using the xylose to produce organic acids,the xylA gene from Escherichia coli K-12,encoding xylose isomerase,was integrated in the expression vector of pXMJ19.The gene was expressed in the Corynebacterium glutamicum ATCC13032Δldh strain.The recombinant strain was capable of growth on xylose as a sole carbon source,the xylose consumption rate was 0.54 g/( L·h ).The xylose isomerase activity reached 0.54 U/mL.In medium containing glucose and xylose, the recombinant strain consumed glucose first, after the glucose was consumped entirely, the effective utilization of xylose was started.The yield of succinate was ( 0.62 ± 0.003) g/g during the two-stage fermentation on xylose as a sole carbon source.