CAJ | 학술논문

以从短穗竹（ Brachystachyum densiflorum）茎秆中分离获得的竹黄菌（ Shiraia sp.S8）菌株为出发菌株，以.5％～2.5％甲基磺酸乙酯（ EMS）处理竹黄菌悬浮孢子～30 min，结合平板初筛和高效液相色谱（ HPLC）分析进行复筛。经诱变筛选得到高产菌株，发现其竹红菌甲素产量达到.8 mg/L，与原始出发菌株相比提高了.4％，且遗传稳定性良好，具有较高的医药及工业应用价值。
이종단수죽（ Brachystachyum densiflorum）경간중분리획득적죽황균（ Shiraia sp.S8）균주위출발균주，이.5％～2.5％갑기광산을지（ EMS）처리죽황균현부포자～30 min，결합평판초사화고효액상색보（ HPLC）분석진행복사。경유변사선득도고산균주，발현기죽홍균갑소산량체도.8 mg/L，여원시출발균주상비제고료.4％，차유전은정성량호，구유교고적의약급공업응용개치。
One isolated strain Shiraia sp. S8 from the stems of Brachystachyum densiflorum was breeding candidates for higher production of hypocrellin A ( HA) by ethyl methane sulphonate ( EMS) mutation. EMS at 0.5% to 2.5% was used to treat the suspension spores for 10 min to 30 min.Through the combined methods with the preliminary screening on the diameter of the red pigment ring and HPLC,the mutant 10-5 with higher HA yield was obtained and the production was verified to be genetically stable.HA yield by mutant 10-5 was increased by 46.4% than that of the original strain.The EMS mutant had industrial potential and pharmaceutical importance for HA.