生物加工过程
生物加工過程
생물가공과정
CHINESE JOURNAL OF BIOPROCESS ENGINEERING
2014年
2期
17-23
,共7页
黑曲霉%驯化%菊粉酶%诱变%响应面
黑麯黴%馴化%菊粉酶%誘變%響應麵
흑곡매%순화%국분매%유변%향응면
Aspergillus niger%domestication%inulinase%mutation%response surface methodology
为获得高产菊粉酶的黑曲霉菌株，以Aspergillus niger YH 1为出发菌株，经过亚硝基胍（ NTG）诱变，以高温高菊芋粉相结合的方式进行梯度驯化，选育出一株产菊粉酶菌株YH 3，并运用响应面实验方法对该菌株的培养基进行优化。确定了最佳培养基组成：菊芋粉.2 g/L、豆饼粉 g/L、蔗糖酯.9 g/L、NaCl 5.5 g/L。发现内切菊粉酶活力（ I）由.9 U/mL提高到.0 U/mL，比出发菌株提高了.7倍。研究证明蔗糖酯对于黑曲霉YH 3发酵产菊粉酶是一种有效的促进剂。
為穫得高產菊粉酶的黑麯黴菌株，以Aspergillus niger YH 1為齣髮菌株，經過亞硝基胍（ NTG）誘變，以高溫高菊芋粉相結閤的方式進行梯度馴化，選育齣一株產菊粉酶菌株YH 3，併運用響應麵實驗方法對該菌株的培養基進行優化。確定瞭最佳培養基組成：菊芋粉.2 g/L、豆餅粉 g/L、蔗糖酯.9 g/L、NaCl 5.5 g/L。髮現內切菊粉酶活力（ I）由.9 U/mL提高到.0 U/mL，比齣髮菌株提高瞭.7倍。研究證明蔗糖酯對于黑麯黴YH 3髮酵產菊粉酶是一種有效的促進劑。
위획득고산국분매적흑곡매균주，이Aspergillus niger YH 1위출발균주，경과아초기고（ NTG）유변，이고온고국우분상결합적방식진행제도순화，선육출일주산국분매균주YH 3，병운용향응면실험방법대해균주적배양기진행우화。학정료최가배양기조성：국우분.2 g/L、두병분 g/L、자당지.9 g/L、NaCl 5.5 g/L。발현내절국분매활력（ I）유.9 U/mL제고도.0 U/mL，비출발균주제고료.7배。연구증명자당지대우흑곡매YH 3발효산국분매시일충유효적촉진제。
In order to acquire a high-yield Aspergillus niger strain for producing inulinase,the original strain Aspergillus niger YH-1 was treated with N-methyl-N′-nitro-soguanidine ( NTG ) and domesticated by combining high temperature and Jerusalem artichoke powder, a inulinase production strain YH-3 was selected.The culture medium of YH-3 was optimized by response surface methodology.The results showed that the optimum culture medium components were determined:Jerusalem artichoke flour 25.2 g/L, soybean cake powder 40 g/L, sucrose ester 4.9 g/L and NaCl 5.5 g/L.Under the optimum conditions, the yield of inulinase was increased from 60.9 U/mL to 165.0 U/mL, increased 1.7 times compared with the starting strain.The research proved that sucrose ester was an effective accelerator for Aspergillus niger YH-3 producting inulinase.
