CAJ | 학술논문

目的：确定光动力治疗对尖锐湿疣( CA)人乳头瘤病毒( HPV)的影响。方法：采用实时荧光定量聚合酶链反应( FQ-PCR)检测65例CA患者光动力治疗前、治疗3次后HPV DNA拷贝数。随访3个月观察复发情况，并分析复发与HPV DNA载量的关系。结果：3次治疗后HPV DNA拷贝数(3.10×103)较治疗前(5.12×106)显著下降(P<0.05)；复发患者(12例)治疗前平均拷贝数(4.62×107)高于未复发患者(6.75×104)(P<0.05)。结论：光动力治疗CA能显著降低HPV病毒载量；患者疣体中HPV DNA载量与复发有关。
목적：학정광동력치료대첨예습우( CA)인유두류병독( HPV)적영향。방법：채용실시형광정량취합매련반응( FQ-PCR)검측65례CA환자광동력치료전、치료3차후HPV DNA고패수。수방3개월관찰복발정황，병분석복발여HPV DNA재량적관계。결과：3차치료후HPV DNA고패수(3.10×103)교치료전(5.12×106)현저하강(P<0.05)；복발환자(12례)치료전평균고패수(4.62×107)고우미복발환자(6.75×104)(P<0.05)。결론：광동력치료CA능현저강저HPV병독재량；환자우체중HPV DNA재량여복발유관。
Objective:To determine the effect of photodynamic therapy on human papilloma virus ( HPV) DNA load in the lesion of condyloma acuminatum ( CA) . Methods:The number of HPV DNA copy in 65 pa-tients with ( CA) were detected by Real-time fluorescence quantitative polymerase chain reaction ( FQ-PCR) before the treatment and after 3 times treatment. The patients were followed-up for relapse after 3 months treat-ment, The correlation between the relapse and the number of HPV DNA copy were analyzed. Results:The av-erage HPV DNA copy number was ( 5. 12 × 106 ) before the treatment and the HPV DNA copy number was (3.10×103) after the treatment, with a significant difference (P<0.05). A total of 12 patients were relapsed. The average HPV DNA copy number in the relapsed patients was (4.62×107) before the treatment, which was higher than (6.75×104) in the non-relapsed patients before the treatment (P<0.05). Conclusion:Photody-namic therapy can significantly reduce HPV load in the lesion of CA. The recurrence of CA was correlated with the HPV-DNA load before the treatment.