中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
4期
80-81,85
,共3页
元胡止痛软胶囊%没食子酸%高效液相色谱法
元鬍止痛軟膠囊%沒食子痠%高效液相色譜法
원호지통연효낭%몰식자산%고효액상색보법
Yuanhu Zhitong Soft Capsule%gallic acid%HPLC
目的：建立元胡止痛软胶囊中没食子酸含量的高效液相色谱测定方法。方法色谱柱：Unitary C18(250 mm×4.6 mm,5μm)；流动相：甲醇-0.5%磷酸(5∶95)；流速：1.0 mL/min；柱温：30℃,检测波长：254 nm。结果没食子酸在0.0248～0.496μg范围内与峰面积呈良好的线性关系,r＝0.9999,日内精密度、重复性、稳定性的RSD分别为0.27%、0.21%、1.40%,平均加样回收率为99.81%,RSD＝0.72%。结论本方法精确可靠,可作为元胡止痛软胶囊中没食子酸含量测定的方法。
目的：建立元鬍止痛軟膠囊中沒食子痠含量的高效液相色譜測定方法。方法色譜柱：Unitary C18(250 mm×4.6 mm,5μm)；流動相：甲醇-0.5%燐痠(5∶95)；流速：1.0 mL/min；柱溫：30℃,檢測波長：254 nm。結果沒食子痠在0.0248～0.496μg範圍內與峰麵積呈良好的線性關繫,r＝0.9999,日內精密度、重複性、穩定性的RSD分彆為0.27%、0.21%、1.40%,平均加樣迴收率為99.81%,RSD＝0.72%。結論本方法精確可靠,可作為元鬍止痛軟膠囊中沒食子痠含量測定的方法。
목적：건립원호지통연효낭중몰식자산함량적고효액상색보측정방법。방법색보주：Unitary C18(250 mm×4.6 mm,5μm)；류동상：갑순-0.5%린산(5∶95)；류속：1.0 mL/min；주온：30℃,검측파장：254 nm。결과몰식자산재0.0248～0.496μg범위내여봉면적정량호적선성관계,r＝0.9999,일내정밀도、중복성、은정성적RSD분별위0.27%、0.21%、1.40%,평균가양회수솔위99.81%,RSD＝0.72%。결론본방법정학가고,가작위원호지통연효낭중몰식자산함량측정적방법。
Objective To establish the determination method of gallic acid in Yuanhu Zhitong Soft Capsule by HPLC. Methods Chromatography was performed on a Unitary C18 column (250 mm× 4.6 mm, 5 μm) at 254 nm. The mobile phase consisted of a mixture of methanol and 0.5%phosphate acid (5∶95) at flow rate of 1.0 mL/min. Results The standard curve of linear relationship was 0.024 8-0.496 μg and r=0.999 9. The RSD of precision, repeatability and stability was 0.27%, 0.21%, 1.40%, respectively. The average recovery was 99.81%, RSD was 0.72%. Conclusion The established method was accurate and reliable for the determination of gallic acid in Yuanhu Zhitong Soft Capsule.
