CAJ | 학술논문

目的：考察复方中药制剂发酵过程中的影响因素,通过单因素试验及响应面法,确定其最佳发酵工艺。方法通过控制复方中药发酵过程中的因素(如发酵菌种、发酵时间、发酵温度、接种量等),采用高效液相色谱法(HPLC),以其总峰面积提高率为指标,对发酵前后醇提液进行监控和对比评价,并采用响应面法确定其最佳发酵工艺。结果经单因素试验及响应面法确定的最佳发酵工艺为：以SZ-2为发酵菌种,温度33℃,接种量4%,发酵3.5 d,其总峰面积提高率平均可达31.24%。结论可以用HPLC指纹图谱对不同影响因素下的复方中药制剂发酵进行对比及评价,并通过响应面法明确其最佳发酵工艺,为其发酵工艺开发提供了参考。
목적：고찰복방중약제제발효과정중적영향인소,통과단인소시험급향응면법,학정기최가발효공예。방법통과공제복방중약발효과정중적인소(여발효균충、발효시간、발효온도、접충량등),채용고효액상색보법(HPLC),이기총봉면적제고솔위지표,대발효전후순제액진행감공화대비평개,병채용향응면법학정기최가발효공예。결과경단인소시험급향응면법학정적최가발효공예위：이SZ-2위발효균충,온도33℃,접충량4%,발효3.5 d,기총봉면적제고솔평균가체31.24%。결론가이용HPLC지문도보대불동영향인소하적복방중약제제발효진행대비급평개,병통과향응면법명학기최가발효공예,위기발효공예개발제공료삼고。
Objective To investigate the factors that influence the fermentation process of compound Chinese medicine and determining the optimum fermentation with single factor experiment and response surface methodology. Methods Through controlling the factors in the fermentation process of compound Chinese medicine (such as fermentation bacteria, fermentation time, fermentation temperature, inoculum amount, etc.), with its increase rate of total peak area as evaluation indicator, the alcohol extracts before and after fermentation were monitored and comparative evaluated by HPLC, and the optimum fermentation process was determined by response surface methodology. Results The fermentation process optimized by single factor experiment and response surface methodology was as follows:SZ-2 strain served as the fermentation bacteria, temperature was 33 ℃, inoculum amount was 4%, and time was 3.5 d, the average increase rate of total peak area was 31.24%. Conclusion HPLC can be used to identify and evaluate the fermentation of compound Chinese medicine under the different factors, and to clarify the optimal fermentation process by response surface methodology, which provide reference for the development of fermentation process.