中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
4期
63-66
,共4页
魏文静%张冠庆%刘世杰%刘同祥
魏文靜%張冠慶%劉世傑%劉同祥
위문정%장관경%류세걸%류동상
通关藤苷H%Lewis肺癌%抑瘤率%脾指数%胸腺指数%白细胞介素-2%白细胞介素-10%小鼠
通關籐苷H%Lewis肺癌%抑瘤率%脾指數%胸腺指數%白細胞介素-2%白細胞介素-10%小鼠
통관등감H%Lewis폐암%억류솔%비지수%흉선지수%백세포개소-2%백세포개소-10%소서
tenacissoside H%Lewis lung cancer%tumor inhibition rate%spleen index%thymus index%IL-2%IL-10%mice
目的：研究通关藤苷H对小鼠Lewis肺癌的抑制作用,观察其对荷瘤小鼠免疫功能的影响。方法小鼠右腋皮下注射Lewis肺癌细胞,选取接种成功的荷瘤小鼠30只,随机分为模型组、环磷酰胺组、通关藤苷H组,每组10只。3组均从接种后第3日开始,每隔2d,分别给予腹腔注射生理盐水、环磷酰胺、通关藤苷H,0.2mL/只。第21日,摘眼球取血,取肿瘤组织、脾、胸腺并分别称重,计算抑瘤率、脾指数和胸腺指数；采用ELISA法检测血清中白细胞介素-2(IL-2)、白细胞介素-10(IL-10)的含量。结果环磷酰胺组、通关藤苷H组的瘤质量均低于模型组,差异有统计学意义(P＜0.05),抑瘤率分别为54.12%、25.68%；通关藤苷H组的胸腺指数与脾指数升高,环磷酰胺组则下降较为显著。ELISA法检测结果显示,与模型组比较,通关藤苷H组的IL-2水平明显升高,IL-10水平显著降低。结论通关藤苷H可以抑制Lewis肺癌细胞生长和转移,调节免疫细胞因子IL-2、IL-10的表达水平,提高荷瘤小鼠的免疫功能。
目的：研究通關籐苷H對小鼠Lewis肺癌的抑製作用,觀察其對荷瘤小鼠免疫功能的影響。方法小鼠右腋皮下註射Lewis肺癌細胞,選取接種成功的荷瘤小鼠30隻,隨機分為模型組、環燐酰胺組、通關籐苷H組,每組10隻。3組均從接種後第3日開始,每隔2d,分彆給予腹腔註射生理鹽水、環燐酰胺、通關籐苷H,0.2mL/隻。第21日,摘眼毬取血,取腫瘤組織、脾、胸腺併分彆稱重,計算抑瘤率、脾指數和胸腺指數；採用ELISA法檢測血清中白細胞介素-2(IL-2)、白細胞介素-10(IL-10)的含量。結果環燐酰胺組、通關籐苷H組的瘤質量均低于模型組,差異有統計學意義(P＜0.05),抑瘤率分彆為54.12%、25.68%；通關籐苷H組的胸腺指數與脾指數升高,環燐酰胺組則下降較為顯著。ELISA法檢測結果顯示,與模型組比較,通關籐苷H組的IL-2水平明顯升高,IL-10水平顯著降低。結論通關籐苷H可以抑製Lewis肺癌細胞生長和轉移,調節免疫細胞因子IL-2、IL-10的錶達水平,提高荷瘤小鼠的免疫功能。
목적：연구통관등감H대소서Lewis폐암적억제작용,관찰기대하류소서면역공능적영향。방법소서우액피하주사Lewis폐암세포,선취접충성공적하류소서30지,수궤분위모형조、배린선알조、통관등감H조,매조10지。3조균종접충후제3일개시,매격2d,분별급여복강주사생리염수、배린선알、통관등감H,0.2mL/지。제21일,적안구취혈,취종류조직、비、흉선병분별칭중,계산억류솔、비지수화흉선지수；채용ELISA법검측혈청중백세포개소-2(IL-2)、백세포개소-10(IL-10)적함량。결과배린선알조、통관등감H조적류질량균저우모형조,차이유통계학의의(P＜0.05),억류솔분별위54.12%、25.68%；통관등감H조적흉선지수여비지수승고,배린선알조칙하강교위현저。ELISA법검측결과현시,여모형조비교,통관등감H조적IL-2수평명현승고,IL-10수평현저강저。결론통관등감H가이억제Lewis폐암세포생장화전이,조절면역세포인자IL-2、IL-10적표체수평,제고하류소서적면역공능。
Objective To study the anti-tumor effect of tenacissoside H on Lewis lung cancer mice, and explore its impacts on immune function of tumor-bearing mice. Methods Mice was injected Lewis lung cancer cells subcutaneously to the right axilla. Thirty successful tumor-bearing mice were randomly divided into model group, the cyclophosphamide (CTX) group and tenacissoside H group, 10 mice in each group. Three days after inoculation, mice were intraperitoneally injected by normal saline, CTX and tenacissoside H respectively every two days, 0.2 mL in each mouse. On the 21st day, the eyeballs were extracted and blood was drawn, tumor tissue, spleen and thymus were taken and weighted to calculate tumor inhibition rate, spleen index and thymus index, and the contents of IL-2 and IL-10 were detected by ELISA. Results The tumor weights of CTX group and tenacissoside H group were lower than that of the model group with significant difference (P<0.05), and the tumor inhibition rates were 54.12%, 25.68%. The thymus index and spleen index of tenacissoside H group increased, but that of CTX group decreased significantly. Compared with the model group, the IL-2 level of tenacissoside H group was significantly increased, while the IL-10 level decreased. Conclusion Tenacissoside H can inhibit growth and metastasis of Lewis lung cancer, regulate the expression of IL-2 and IL-10, and improve the immune function of tumor-bearing mice.
