中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
4期
59-62,66
,共5页
段永强%程卫东%杜娟%杨晓轶%刘靓%巩子汉%朱立鸣%成映霞%李兰珍
段永彊%程衛東%杜娟%楊曉軼%劉靚%鞏子漢%硃立鳴%成映霞%李蘭珍
단영강%정위동%두연%양효질%류정%공자한%주립명%성영하%리란진
脾气虚证%神经肽Y%血管活性肠肽%丝裂原活化蛋白激酶%四君子汤%大鼠
脾氣虛證%神經肽Y%血管活性腸肽%絲裂原活化蛋白激酶%四君子湯%大鼠
비기허증%신경태Y%혈관활성장태%사렬원활화단백격매%사군자탕%대서
spleen-qi deficiency%NPY%vasoactive intestinal peptide%Mapk%Sijunzi decoction%rats
目的:观察脾气虚大鼠血清、小肠神经肽Y (NPY)和血管活性肠肽(VIP)含量,小肠组织丝裂原活化蛋白激酶14(Mapk14)mRNA表达的变化及益气健脾中药的干预效应,以揭示脾气虚证发生的内在机制。方法受试动物随机分为空白组、模型组(7、14、21 d组)、益气健脾组,每组10只。除空白组外,其余各组采用大黄法、力竭法及饥饿法复合法建立脾气虚证大鼠模型,造模同时益气健脾组每日给予四君子汤20 g/kg干预21 d,各组大鼠分时段采用放免法测定小肠、血清NPY和VIP含量,采用实时荧光定量PCR检测小肠组织Mapk14 mRNA表达水平。结果与空白组比较,模型组大鼠血清及小肠组织 NPY 含量降低(P<0.05),VIP 含量升高(P<0.05),小肠组织Mapk14 mRNA相对表达量升高(P<0.05),且以模型21 d组显著;与模型21 d组比较,益气健脾组大鼠血清及小肠组织NPY含量升高(P<0.05),VIP含量降低(P<0.05),小肠组织Mapk14 mRNA相对表达量降低(P<0.05)。结论益气健脾中药具有调节脑肠肽NPY、VIP分泌及抑制小肠组织Mapk14 mRNA异常表达的作用。
目的:觀察脾氣虛大鼠血清、小腸神經肽Y (NPY)和血管活性腸肽(VIP)含量,小腸組織絲裂原活化蛋白激酶14(Mapk14)mRNA錶達的變化及益氣健脾中藥的榦預效應,以揭示脾氣虛證髮生的內在機製。方法受試動物隨機分為空白組、模型組(7、14、21 d組)、益氣健脾組,每組10隻。除空白組外,其餘各組採用大黃法、力竭法及饑餓法複閤法建立脾氣虛證大鼠模型,造模同時益氣健脾組每日給予四君子湯20 g/kg榦預21 d,各組大鼠分時段採用放免法測定小腸、血清NPY和VIP含量,採用實時熒光定量PCR檢測小腸組織Mapk14 mRNA錶達水平。結果與空白組比較,模型組大鼠血清及小腸組織 NPY 含量降低(P<0.05),VIP 含量升高(P<0.05),小腸組織Mapk14 mRNA相對錶達量升高(P<0.05),且以模型21 d組顯著;與模型21 d組比較,益氣健脾組大鼠血清及小腸組織NPY含量升高(P<0.05),VIP含量降低(P<0.05),小腸組織Mapk14 mRNA相對錶達量降低(P<0.05)。結論益氣健脾中藥具有調節腦腸肽NPY、VIP分泌及抑製小腸組織Mapk14 mRNA異常錶達的作用。
목적:관찰비기허대서혈청、소장신경태Y (NPY)화혈관활성장태(VIP)함량,소장조직사렬원활화단백격매14(Mapk14)mRNA표체적변화급익기건비중약적간예효응,이게시비기허증발생적내재궤제。방법수시동물수궤분위공백조、모형조(7、14、21 d조)、익기건비조,매조10지。제공백조외,기여각조채용대황법、력갈법급기아법복합법건립비기허증대서모형,조모동시익기건비조매일급여사군자탕20 g/kg간예21 d,각조대서분시단채용방면법측정소장、혈청NPY화VIP함량,채용실시형광정량PCR검측소장조직Mapk14 mRNA표체수평。결과여공백조비교,모형조대서혈청급소장조직 NPY 함량강저(P<0.05),VIP 함량승고(P<0.05),소장조직Mapk14 mRNA상대표체량승고(P<0.05),차이모형21 d조현저;여모형21 d조비교,익기건비조대서혈청급소장조직NPY함량승고(P<0.05),VIP함량강저(P<0.05),소장조직Mapk14 mRNA상대표체량강저(P<0.05)。결론익기건비중약구유조절뇌장태NPY、VIP분비급억제소장조직Mapk14 mRNA이상표체적작용。
Objective To investigate the effect of Yiqi Jianpi herb on content of NPY, VIP and expression of Mapk14 mRNA of rats with spleen-qi deficiency. Methods Rats were randomly divided into normal groups, model group (observed on 7 d, 14 d and 21 d), treatment group of Yiqi Jianpi herb, 10 rats in each group. The spleen-qi deficient model rats were made by rhubarb, exhaustive and hungry method, and treatment group was treated with Sijunzi decoction (20 g/kg) for 21 d, then the content of NPY, VIP in serum and small intestine were evaluated with radioimmunoassay, and expression of Mapk14 mRNA in small intestine tissue was evaluated with real time fluorescent quantitative PCR. Results In model group, content of NPY was much lower than that of normal group (P<0.05), content of VIP and relative expression of Mapk14 mRNA in small intestine tissue were much higher than that of normal group (P<0.05), the difference was obvious in 21 d group. Compared with model 21 d group, content of NPY was increased (P<0.05), content of VIP and relative expression of Mapk14 mRNA in small intestine tissue were decreased (P<0.05) in treatment group. Conclusion Yiqi Jianpi herb has functions of adjusting NPY, VIP secretion and inhibiting abnormal expression of Mapk14 mRNA.