CAJ | 학술논문

目的：观察肠艾舒对荷瘤小鼠CT-26大肠癌的抑瘤作用及其对肿瘤组织Ki67、PCNA表达的影响。方法将40只BALB/c小鼠随机分为4组,右前腋下接种CT-26细胞造模,空白组(NS 0.6 mL)、化疗组(卡培他滨205.5 mg/kg)、中药高剂量组(肠艾舒51.38 g/kg)、中药低剂量组(肠艾舒17.13 g/kg)隔日灌胃,15 d后计算抑瘤率,免疫组化法检测肿瘤组织Ki67、PCNA的表达。结果与空白组比较,化疗组、中药高剂量组、中药低剂量组抑瘤率分别为43.35%、29.48%、13.30%；与空白组比较,各治疗组Ki67、PCNA标记指数差异均有统计学意义(P＜0.05,P＜0.01)。结论肠艾舒对小鼠CT-26大肠癌的体内生长有一定抑制作用,可能与其下调肿瘤组织Ki67、PCNA表达有关。
목적：관찰장애서대하류소서CT-26대장암적억류작용급기대종류조직Ki67、PCNA표체적영향。방법장40지BALB/c소서수궤분위4조,우전액하접충CT-26세포조모,공백조(NS 0.6 mL)、화료조(잡배타빈205.5 mg/kg)、중약고제량조(장애서51.38 g/kg)、중약저제량조(장애서17.13 g/kg)격일관위,15 d후계산억류솔,면역조화법검측종류조직Ki67、PCNA적표체。결과여공백조비교,화료조、중약고제량조、중약저제량조억류솔분별위43.35%、29.48%、13.30%；여공백조비교,각치료조Ki67、PCNA표기지수차이균유통계학의의(P＜0.05,P＜0.01)。결론장애서대소서CT-26대장암적체내생장유일정억제작용,가능여기하조종류조직Ki67、PCNA표체유관。
Objective To observe the anti-tumor action of Chang'aishu and its influence on the expression of the Ki67 and PCNA. Methods Forty Balb/c mice with CT-26 colorectal cancer were randomly divided into four groups, including the control group (normal saline, 0.6 mL), the chemotherapy group (Xeloda 205.5 mg/kg), the high dose group (Chang'aishu 51.38 g/kg) and the low dose group (Chang'aishu 17.13 g/kg). Each group of mice was treated with intragastric administration every two days. After 15 days, the anti-tumor rate was calculated and the expression of Ki67 and PCNA were analyzed by immunohistochemistry. Results Compared with the control group, inhibitive rate of the chemotherapy group, the high dose group and the low dose group was 43.35%, 29.48% and 13.30%, respectively. Compared with the control group, the treatment group showed statistical significant difference in Ki67 and PCNA (P<0.05, P<0.01). Conclusion Chang'aishu had inhibition effect on the growth of colorectal cancer, which may be related to down-regulating the expression of Ki67 and PCNA.