CAJ | 학술논문

目的：观察中药复方糖痹康对糖尿病周围神经病变大鼠血浆β-内啡肽(β-EP)含量的影响,探讨其镇痛及神经保护机制。方法采用高脂饲料和小剂量链脲佐菌素(STZ)诱发2型糖尿病大鼠动物模型,造模后随机分为模型组、甲钴胺组及糖痹康高、中、低剂量组,另设10只大鼠为正常组。模型组和正常组给予等量蒸馏水,各治疗组给予相应药物灌胃,每4周检测体质量、空腹血糖,16周后取大鼠一侧坐骨神经,光镜观察各组大鼠坐骨神经病理变化,并用放射免疫法检测大鼠血浆β-EP变化。结果治疗16周后,糖尿病大鼠体质量、血糖明显改善。与模型组比较,甲钴胺组及糖痹康各剂量组能明显改善糖尿病周围神经病变大鼠坐骨神经病理组织学损伤程度。与正常组比较,模型组β-EP表达显著降低(P＜0.01)；与模型组比较,糖痹康中、高剂量组显著增加β-EP表达(P＜0.01)。结论糖痹康能降低糖尿病大鼠血糖,上调糖尿病周围神经病变大鼠血浆β-EP水平,对坐骨神经的形态具有保护作用。
목적：관찰중약복방당비강대당뇨병주위신경병변대서혈장β-내배태(β-EP)함량적영향,탐토기진통급신경보호궤제。방법채용고지사료화소제량련뇨좌균소(STZ)유발2형당뇨병대서동물모형,조모후수궤분위모형조、갑고알조급당비강고、중、저제량조,령설10지대서위정상조。모형조화정상조급여등량증류수,각치료조급여상응약물관위,매4주검측체질량、공복혈당,16주후취대서일측좌골신경,광경관찰각조대서좌골신경병리변화,병용방사면역법검측대서혈장β-EP변화。결과치료16주후,당뇨병대서체질량、혈당명현개선。여모형조비교,갑고알조급당비강각제량조능명현개선당뇨병주위신경병변대서좌골신경병리조직학손상정도。여정상조비교,모형조β-EP표체현저강저(P＜0.01)；여모형조비교,당비강중、고제량조현저증가β-EP표체(P＜0.01)。결론당비강능강저당뇨병대서혈당,상조당뇨병주위신경병변대서혈장β-EP수평,대좌골신경적형태구유보호작용。
Objective To observe the effect of Chinese herbal compound Tangbikang on the level of plasma β-EP of diabetic peripheral neuropathy rats, and to discuss its mechanism of neuroprotection and abirritation. Methods STZ and feeding high fat forage induced diabetic rats were randomly divided into diabetic model group, Mecobalamin group, Tangbikang high dose group, Tangbikang medium dose group, Tangbikang low dose group, with 10 normal rats as control. The control group and model group were daily gavaged with distilled water, Tangbikang groups were given Tangbikang with different dose. Mecobalamin was used as the control medicine. Body weight and FBG was detected every 4 weeks, rats’ unilateral sciatic nerve were taken out at the 16th week. Pathological variation of sciatic nerve was presented by HE staining, and radioimmunoassay method was used to detect the levels of plasma β-EP of diabetic peripheral neuropathy of rats. Results The weight and FBG had obviously changed in the 16th week. Compared with the model group, pathological injury of diabetic peripheral neuropathy rats in the Tangbikang groups were significantly improved. The level ofβ-EP of model group was significantly decreased (P<0.01) compared with normal group. Tangbikang medium and high dose group significantly increased the level of plasma β-EP (P<0.01). Conclusion Chinese herbal compound Tangbikang could reduce the fasting blood glucose, protect nervous system and promote the level of plasmaβ-EP of diabetic peripheral neuropathy rats.