健康之路
健康之路
건강지로
HEALTH
2014年
4期
9-9,10
,共2页
高效液相色谱法%外伤如意膏%盐酸小檗碱
高效液相色譜法%外傷如意膏%鹽痠小檗堿
고효액상색보법%외상여의고%염산소벽감
HPLC%Trauma wishful cream%Berberine
目的：为了能够更好的控制外伤如意膏制剂的中盐酸小檗碱的含量,本文就 HPLC 测定外伤如意膏中盐酸小檗碱的含量进行了浅显的研究和探讨.方法：高效液相色谱法,采用 CAPCELL PAK C18 MGⅡ色谱柱,以乙腈-0．1％磷酸溶液(每100ml 加十二烷基磺酸钠0．1 g)为流动相,流速为1 ml/min,柱温为30℃,检测波长为265 nm.结果：当盐酸小檗碱的含量控制在0．0503μg~0．4026μg 范围之内时,与峰面积的线性关系最为理想,r＝1,平均回收率为98．4％,相对标准偏差值(RSD)＝2．2％(n＝9).结论：HPLC 法应用于外伤如意膏中盐酸小檗碱含量测定的操作便捷,精准性高,值得推广.
目的：為瞭能夠更好的控製外傷如意膏製劑的中鹽痠小檗堿的含量,本文就 HPLC 測定外傷如意膏中鹽痠小檗堿的含量進行瞭淺顯的研究和探討.方法：高效液相色譜法,採用 CAPCELL PAK C18 MGⅡ色譜柱,以乙腈-0．1％燐痠溶液(每100ml 加十二烷基磺痠鈉0．1 g)為流動相,流速為1 ml/min,柱溫為30℃,檢測波長為265 nm.結果：噹鹽痠小檗堿的含量控製在0．0503μg~0．4026μg 範圍之內時,與峰麵積的線性關繫最為理想,r＝1,平均迴收率為98．4％,相對標準偏差值(RSD)＝2．2％(n＝9).結論：HPLC 法應用于外傷如意膏中鹽痠小檗堿含量測定的操作便捷,精準性高,值得推廣.
목적：위료능구경호적공제외상여의고제제적중염산소벽감적함량,본문취 HPLC 측정외상여의고중염산소벽감적함량진행료천현적연구화탐토.방법：고효액상색보법,채용 CAPCELL PAK C18 MGⅡ색보주,이을정-0．1％린산용액(매100ml 가십이완기광산납0．1 g)위류동상,류속위1 ml/min,주온위30℃,검측파장위265 nm.결과：당염산소벽감적함량공제재0．0503μg~0．4026μg 범위지내시,여봉면적적선성관계최위이상,r＝1,평균회수솔위98．4％,상대표준편차치(RSD)＝2．2％(n＝9).결론：HPLC 법응용우외상여의고중염산소벽감함량측정적조작편첩,정준성고,치득추엄.
Objective:To be able to better wishful paste formulations of berberine hydrochloride,this article by HPLC trauma wishful paste berberine hydrochloride were plain study and discussion.Methods:HPLC,using CAPCELL PAK C1 8 MG Ⅱ column with acetonitrile-0.1 % phosphoric acid(sodium dodecyl sulfate per 100ml plus 0. 1 g)as the mobile phase at a flow rate of 1 ml/min,column temperature is 30℃,the detection wavelength was 265nm.Result:When berberine hydrochloride control ed 0. 0503μg~0.4026μg range,the linear relationship between peak area is ideal,r=1 ,the average recovery was 98.4% and the relative standard deviation(RSD)=2.2%(n=9). Conclusion:HPLC applied trauma wishful cream determination of berberine hydrochloride in easy operation,high precision,worthy of promotion.
