健康之路
健康之路
건강지로
HEALTH
2014年
4期
6-7
,共2页
张韦娜%朱沛韶%刘基柱%乔丽
張韋娜%硃沛韶%劉基柱%喬麗
장위나%주패소%류기주%교려
UPLC%复方丹参片%三七
UPLC%複方丹參片%三七
UPLC%복방단삼편%삼칠
UPLC%Fufang Danshen Tablets%Panax notoginseng
目的：建立利用 UPLC 同时测定复方丹参片中4种活性成分含量的方法.方法：采用 ACQUITY UPLC BEH C-18色谱柱；乙腈(A)-水(B)为流动相二元梯度洗脱,检测波长203nm,柱温30℃,流速0．2ml/min,测定同一厂家10批样品以及市售10厂家样品中4种皂苷的含量.结果：4种活性成分在所建浓度范围内线性关系良好,r 均大于0．999.结论：所建立的 UPLC 方法可快速、简单地对复方丹参片中4种活性成分进行含量测定.
目的：建立利用 UPLC 同時測定複方丹參片中4種活性成分含量的方法.方法：採用 ACQUITY UPLC BEH C-18色譜柱；乙腈(A)-水(B)為流動相二元梯度洗脫,檢測波長203nm,柱溫30℃,流速0．2ml/min,測定同一廠傢10批樣品以及市售10廠傢樣品中4種皂苷的含量.結果：4種活性成分在所建濃度範圍內線性關繫良好,r 均大于0．999.結論：所建立的 UPLC 方法可快速、簡單地對複方丹參片中4種活性成分進行含量測定.
목적：건립이용 UPLC 동시측정복방단삼편중4충활성성분함량적방법.방법：채용 ACQUITY UPLC BEH C-18색보주；을정(A)-수(B)위류동상이원제도세탈,검측파장203nm,주온30℃,류속0．2ml/min,측정동일엄가10비양품이급시수10엄가양품중4충조감적함량.결과：4충활성성분재소건농도범위내선성관계량호,r 균대우0．999.결론：소건립적 UPLC 방법가쾌속、간단지대복방단삼편중4충활성성분진행함량측정.
Objective:To establish the methods of determine the contention of four saponins simultaneously in Fufang Danshen Tablets by UPLC.Methods:Fufang Dan-shen Tablets was separated on an Acquity UPLC BEH C18 column.The mobile phase consisted of acetonitrile(B)and water(A)used for separation by gradient elution.The detection wavelength was set at 203 nm.The column temperature was 30℃.The contention was then established for analysis of Fufang Danshen Tablets from 10 corpora-wtions and 10 batches of one corporation.Result:Four saponins had good linearity within the concentration range respectively and r were al greater than 0.999.Four saponinsmere separated completely and determined contention.Conclusion::This UPLC method may greatly improve the separat ion efficiency and analysis speed and rapidly deter-ine the contention of four saponins in the case of four saponins in Fufang Danshen Tablets.
