CAJ | 학술논문

通过1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐( EDC)/N-羟基琥珀酰亚胺( NHS)催化体系使羧甲基壳聚糖( CMCS)交联,制备了新型羧甲基壳聚糖水凝胶.探讨了EDC用量和EDC/NHS质量比对水凝胶特性的影响. CMCS水凝胶具有pH响应特性,在等电位点溶胀率最小.降解实验结果表明,水凝胶浸泡在磷酸盐缓冲溶液中,10 d失重率在15%~45%之间,主要是未交联部分溶解所致.而浸泡在含有0.2 mg/mL溶菌酶的磷酸盐缓冲溶液中,低交联度水凝胶80 h基本降解,高交联度水凝胶不易降解.初步研究了CMCS水凝胶包埋牛血清白蛋白( BSA)的释放行为.
통과1-을기-(3-이갑기안기병기)탄이아알염산염( EDC)/N-간기호박선아알( NHS)최화체계사최갑기각취당( CMCS)교련,제비료신형최갑기각취당수응효.탐토료EDC용량화EDC/NHS질량비대수응효특성적영향. CMCS수응효구유pH향응특성,재등전위점용창솔최소.강해실험결과표명,수응효침포재린산염완충용액중,10 d실중솔재15%~45%지간,주요시미교련부분용해소치.이침포재함유0.2 mg/mL용균매적린산염완충용액중,저교련도수응효80 h기본강해,고교련도수응효불역강해.초보연구료CMCS수응효포매우혈청백단백( BSA)적석방행위.
Novel hydrogels were prepared by crosslinking carboxymethyl chitosan( CMCS) with 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide/N-hydroxysuccinimide ( EDC/NHS ) as catalyst at room temperature. The amount of EDC and EDC/NHS mass ratio revealed to be influencing factors on the reaction. The hydrogels exhibited typical pH-responsive character. Solid-state 13 C nuclear magnetic resonance( NMR) and differential scanning calorimetry( DSC) measurements confirmed the effective crosslinking of carboxymethyl chitosan. A minimum swelling ratio is obtained at the isoelectric point in the pH range of 3-5 . Degradation of hydrogels was carried out at 37 ℃ in phosphate buffered saline( PBS) or in PBS containing 0.2 mg/mL lysozyme. The hydrogels appeared rather stable in PBS for 10 d. The initial mass loss of 15%-45% was assigned to the dis-solution of uncrosslinked CMCS. The hydrogel with low crosslink density was degraded after 80 h in the pre-sence of lysozyme, while the hydrogel with high crosslink density was hardly degraded. A model drug, bovine serum albumin( BSA) was loaded in CMCS hydrogels. Preliminary drug release studies show that the hydrogels are promising carrier of hydrophilic drugs.