CAJ | 학술논문

背景：远程肢体缺血预处理应用到脑缺血-再灌注损伤领域的研究已有一些报道，但肢体缺血后处理应用于该领域报道很少。 　　目的：观察肢体缺血后处理对缺血性脑损伤大鼠侧脑室旁神经干细胞增殖的影响。 　　方法：利用改良线栓法制作局灶性脑缺血大鼠模型，将30只造模成功的SD大鼠随机分为实验组和对照组，每组15只。实验组行远程肢体缺血后处理，对照组不做处理。2组大鼠分别于造模后5，10，15 d处死取脑，处死前1 d每隔8 h腹腔注射50 mg/kg 5-嗅脱氧尿嘧啶核苷1次，共3次。应用免疫组织化学方法检测梗死灶区大鼠脑组织5-嗅脱氧尿嘧啶核苷阳性细胞数。 　　结果与结论：与对照组比较，实验组脑再灌注损伤程度呈现不同程度减轻，行为学评分降低(P<0.05)。实验组各时间点梗死灶周边皮质的5-嗅脱氧尿嘧啶核苷阳性细胞数明显多于对照组(P<0.05)。提示局灶性脑缺血造模后的肢体缺血后处理可以改善大鼠行为学表现，促进了内源性干细胞的激活增殖，对缺血性脑损伤有保护作用。
배경：원정지체결혈예처리응용도뇌결혈-재관주손상영역적연구이유일사보도，단지체결혈후처리응용우해영역보도흔소。 　　목적：관찰지체결혈후처리대결혈성뇌손상대서측뇌실방신경간세포증식적영향。 　　방법：이용개량선전법제작국조성뇌결혈대서모형，장30지조모성공적SD대서수궤분위실험조화대조조，매조15지。실험조행원정지체결혈후처리，대조조불주처리。2조대서분별우조모후5，10，15 d처사취뇌，처사전1 d매격8 h복강주사50 mg/kg 5-후탈양뇨밀정핵감1차，공3차。응용면역조직화학방법검측경사조구대서뇌조직5-후탈양뇨밀정핵감양성세포수。 　　결과여결론：여대조조비교，실험조뇌재관주손상정도정현불동정도감경，행위학평분강저(P<0.05)。실험조각시간점경사조주변피질적5-후탈양뇨밀정핵감양성세포수명현다우대조조(P<0.05)。제시국조성뇌결혈조모후적지체결혈후처리가이개선대서행위학표현，촉진료내원성간세포적격활증식，대결혈성뇌손상유보호작용。
BACKGROUND:There are a few reports addressing application of limb ischemic preconditioning in cerebral ischemia-reperfusion injury, but few reports have addressed limb ischemic postconditioning applied in this field. OBJECTIVE:To explore the effects of limb ischemic postconditioning on the proliferation of neural stem cells of rats after cerebral ischemia. METHODS:Focal stroke models were established in rats, and then 30 rat models were randomly divided into experimental group (n=15) and control group (n=15). Remote limb ischemic postconditioning was applied in the experimental group, but not in the control group. 5-Bromo-2-deoxyuridine (BrdU;50 mg/kg) was injected intraperitoneal y into rat models three times a day, and then the rat brains were respectively obtained at days 5, 10 and 15 days in two groups. The amount of BrdU-positive cells was measured by immunohistochemistry method in two groups. RESULTS AND CONCLUSION:Compared with the control group, the experimental group was significantly decreased in the severity of injury and behavior scores (P<0.05). At various time points, the number of BrdU-positive cells was obviously greater in the experimental group than the control group (P<0.05). Results have indicated that limb ischemic postconditioning can improve behavior of rats and promote the proliferation of neural stem cells, thereby showing neuroprotective effects against cerebral ischemia injury.