CAJ | 학술논문

背景：脐带间充质干细胞具有多向分化能力，但其向成纤维细胞分化研究较少。目的：验证人脐带间充质干细胞向成纤维细胞的分化能力。 　　方法：采用贴壁法分离脐带间充质干细胞，流式细胞仪分析其表面抗原。取第3代脐带间充质干细胞进行成脂成骨诱导分化，以碱性成纤维细胞生长因子诱导脐带间充质干细胞向成纤维细胞分化。 　　结果与结论：贴壁法能稳定从脐带中分离出干细胞，脐带间充质干细胞极低表达 CD31、CD45、CD40、HLA-DR，强表达 CD29、CD90、CD44、CD105。脐带间充质干细胞成脂诱导后油红O染色显示胞浆中充满红色的油滴；成骨诱导后茜素红染色可在细胞密集区见红色的钙结节。碱性成纤维细胞生因子诱导后细胞表达Ⅰ型胶原明显高于对照组。提示贴壁法分离脐带间充质干细胞可靠、纯度高，碱性成纤维细胞生长因子可诱导脐带间充质干细胞向成纤维细胞分化。
배경：제대간충질간세포구유다향분화능력，단기향성섬유세포분화연구교소。목적：험증인제대간충질간세포향성섬유세포적분화능력。 　　방법：채용첩벽법분리제대간충질간세포，류식세포의분석기표면항원。취제3대제대간충질간세포진행성지성골유도분화，이감성성섬유세포생장인자유도제대간충질간세포향성섬유세포분화。 　　결과여결론：첩벽법능은정종제대중분리출간세포，제대간충질간세포겁저표체 CD31、CD45、CD40、HLA-DR，강표체 CD29、CD90、CD44、CD105。제대간충질간세포성지유도후유홍O염색현시포장중충만홍색적유적；성골유도후천소홍염색가재세포밀집구견홍색적개결절。감성성섬유세포생인자유도후세포표체Ⅰ형효원명현고우대조조。제시첩벽법분리제대간충질간세포가고、순도고，감성성섬유세포생장인자가유도제대간충질간세포향성섬유세포분화。
BACKGROUND:The umbilical cord mesenchymal stem cells possess multipotent differentiation capacity, but less research focus on its differentiation into fibroblasts. OBJECTIVE:To investigate the capacity of human umbilical cord mesenchymal stem cells differentiating into fibroblasts. METHODS:Using adherent method, human umbilical cord mesenchymal stem cells were isolated, and flow cytometric analysis of the surface antigen was performed. Passage 3 cells were selected for osteogenic and adipogenic differentiation, and cells differentiated into fibroblasts under the induction of basic fibroblast growth factor. RESULTS AND CONCLUSION:Adherent stem cells were stably isolated from the umbilical cord. Human umbilical cord mesenchymal stem cells lowly expressed CD31, CD45, CD40, HLA-DR, but strongly expressed CD29, CD90, CD44, CD105. Oil red O staining showed red droplets were ful of the cytoplasm after adipogenic induction;alizarin red staining showed red calcium nodules after osteogenic induction. After induced by basic fibroblast growth factor, the type I col agen expression was significantly higher than that in the control group. These findings indicate that the adherent human umbilical cord mesenchymal stem cells are reliably isolated with high purity;basic fibroblast growth factor can induce differentiation of umbilical cord mesenchymal stem cells into fibroblasts.