CAJ | 학술논문

背景：培养体系微环境影响干细胞体外扩增，寻找有效的促进细胞贴壁和增殖的培养方法尤为重要。 　　目的：比较不同细胞培养材质对细胞体外扩增的影响。 　　方法：将5.0×104培养的人脐带源间充质干细胞分别接种于包被/不包被鸟氨酸Corning?聚苯乙烯培养皿或Corning? Cel BIND?表面培养皿中培养，分别观察细胞的贴壁、增殖状态、与细胞黏附和细胞增殖有关的蛋白的表达及细胞标志物的表达。 　　结果与结论：与其他类型的培养皿相比，包被多聚鸟氨酸的Corning? Cel BIND?表面培养皿在24 h内能够促进人脐带源间充质干细胞贴壁，同时也能使培养的人脐带源间充质干细胞较早的进入对数生长期，细胞增殖数量相对较多，虽然对人脐带源间充质干细胞表面标志物CD73， CD90和CD105的表达没有影响，但能促进细胞中黏附蛋白的表达。且Corning? Cel BIND?表面培养皿较Corning?聚苯乙烯培养皿更能促进人脐带源间充质干细胞的贴壁和增殖，同时也对细胞表型的表达无影响。提示Corning? Cel BIND?表面培养皿能够促进细胞吸附，增加细胞数量，且对细胞周期蛋白以及细胞表型的表达没有影响，且包被多聚鸟氨酸能够进一步促进细胞的贴壁和增殖，并稳定表达人脐带源间充质干细胞的细胞表型。
배경：배양체계미배경영향간세포체외확증，심조유효적촉진세포첩벽화증식적배양방법우위중요。 　　목적：비교불동세포배양재질대세포체외확증적영향。 　　방법：장5.0×104배양적인제대원간충질간세포분별접충우포피/불포피조안산Corning?취분을희배양명혹Corning? Cel BIND?표면배양명중배양，분별관찰세포적첩벽、증식상태、여세포점부화세포증식유관적단백적표체급세포표지물적표체。 　　결과여결론：여기타류형적배양명상비，포피다취조안산적Corning? Cel BIND?표면배양명재24 h내능구촉진인제대원간충질간세포첩벽，동시야능사배양적인제대원간충질간세포교조적진입대수생장기，세포증식수량상대교다，수연대인제대원간충질간세포표면표지물CD73， CD90화CD105적표체몰유영향，단능촉진세포중점부단백적표체。차Corning? Cel BIND?표면배양명교Corning?취분을희배양명경능촉진인제대원간충질간세포적첩벽화증식，동시야대세포표형적표체무영향。제시Corning? Cel BIND?표면배양명능구촉진세포흡부，증가세포수량，차대세포주기단백이급세포표형적표체몰유영향，차포피다취조안산능구진일보촉진세포적첩벽화증식，병은정표체인제대원간충질간세포적세포표형。
BACKGROUND:Stem cells expansion technology in vitro is affected by the microenvironment of the culture system. So, to find an effective method is particularly important to promote celladherent and growth. OBJECTIVE:To compare the effects of different culture media on cellexpansion. METHODS:Human umbilical cord mesenchymal stem cells at a density of 5.0×104 cells/cm2 were inoculated into Corning? polystyrene culture dishes coated with or without poly-L-ornithine and Corning? cellBIND culture dishes. celladhesion and proliferation were observed, and expressions of celladhesion proteins and cellmarkers were detected. RESULTS AND CONCLUSION:celladhesion was promoted when cells were cultured in Corning? cellBIND? Surface medium coated with poly-L-ornithine for 24 hours, and the cultured cells grew at the logarithmic phase. The cellproliferation was also enhanced, and the cells expressed celladhesion protein but not the cellmarkers of CD73, CD90, CD105. Corning? cellBIND? Surface medium was superior to Corning? polystyrene culture medium in the improvement of celladhesion and proliferation. Additional y, both of these two media showed no influence on cellphenotype. These findings indicate that Corning? cellBIND? Surface medium can promote celladhesion and proliferation, but shows no effects on cyclin and cellphenotype. This medium coated with poly-L-ornithine can further accelerate celladhesion and proliferation, and stably express cellphenotype of human umbilical cord mesenchymal stem cells.