CAJ | 학술논문

背景：研究表明26RFa在骨形成、痛觉、内分泌、心血管以及能量代谢等方面都有重要的调节作用。目的：观察26RFa在成骨培养体系中对人骨髓间充质干细胞增殖分化的影响。<br>　　方法：通过MTT实验，以此确定26RFa对人骨髓间充质干细胞是否有促增殖作用及其起作用的最大活性浓度；将人骨髓间充质干细胞接种于6孔培养板上，实验分为2组：实验组含有10-11mol/L的26RFa，对照组不含26RFa。取成骨诱导8，12，16 d细胞用碱性磷酸酶试剂盒测定细胞内碱性磷酸酶活性；成骨诱导21，28 d后行茜素红染色和Von Kossa染色，计算每张玻片钙化结节数，Western blot检测cbfa1的表达。<br>　　结果与结论：成骨诱导8，12，16 d后细胞内碱性磷酸酶活性实验组高于对照组(P<0.05，P<0.01，P<0.05)；21，28 d后茜素红染色和Von Kossa矿化结节数实验组均高于对照组；实验组细胞cbfa1表达明显高于对照组(P<0.05)。说明在适宜的培养条件下，26RFa可促进人骨髓间充质干细胞向成骨细胞分化。
배경：연구표명26RFa재골형성、통각、내분비、심혈관이급능량대사등방면도유중요적조절작용。목적：관찰26RFa재성골배양체계중대인골수간충질간세포증식분화적영향。<br>　　방법：통과MTT실험，이차학정26RFa대인골수간충질간세포시부유촉증식작용급기기작용적최대활성농도；장인골수간충질간세포접충우6공배양판상，실험분위2조：실험조함유10-11mol/L적26RFa，대조조불함26RFa。취성골유도8，12，16 d세포용감성린산매시제합측정세포내감성린산매활성；성골유도21，28 d후행천소홍염색화Von Kossa염색，계산매장파편개화결절수，Western blot검측cbfa1적표체。<br>　　결과여결론：성골유도8，12，16 d후세포내감성린산매활성실험조고우대조조(P<0.05，P<0.01，P<0.05)；21，28 d후천소홍염색화Von Kossa광화결절수실험조균고우대조조；실험조세포cbfa1표체명현고우대조조(P<0.05)。설명재괄의적배양조건하，26RFa가촉진인골수간충질간세포향성골세포분화。
BACKGROUND:Studies have shown that 26RFa plays an important regulatory role in bone formation, pain, endocrine, cardiovascular disease and energy metabolism. OBJECTIVE:To observe the effects of 26RFa on the proliferation and differentiation of human bone marrow mesenchymal stem cells. METHODS:In order to obtain the most efficient concentration of 26RFa, human bone marrow mesenchymal stem cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analysis. cells were inoculated into 6-wel plates and then divided into two groups:experimental group treated with 10-11 mol/L 26RFa and control group with no 26RFa. After 8, 12 and 16 days of osteogenic induction, alkaline phosphatase activities in induced cells were detected using alkaline phosphatase kit. After 21 and 28 days of osteogenic induction, alizarin red staining and Von Kossa staining were performed. The number of calcified nodules over each coverslip was calculated, and the expression of cbfa1 protein was detected by western blot assay. RESULTS AND CONCLUSION:After 8, 12, and 16 days of osteogenic induction, the alkaline phosphatase activities were higher in the experimental group than the control group (P<0.05, P<0.01, P<0.05). After 21 and 28 days of osteogenic induction, alizarin red staining and Von Kossa staining showed that the number of calcified nodules was higher in the experimental group than the control group, and the expression of cbfa1 protein was also higher in the experimental group (P<0.05). These findings indicate that 26RFa can promote the osteogenic differentiation of human bone marrow mesenchymal stem cells under appropriate culture conditions.