CAJ | 학술논문

背景：骨髓来源的间充质干细胞含量极低，体外纯化、扩增活性好、分化潜能高的骨髓间充质干细胞，对进一步研究至关重要。 　　目的：进一步验证全骨髓贴壁法体外分离、培养、纯化骨髓间充质干细胞的生物学特性、表型及多向分化潜能。 　　方法：通过全骨髓贴壁法体外分离、培养、纯化大鼠骨髓间充质干细胞，进行形态学观察，流式细胞仪检测细胞标记物表达，分别进行成骨、成脂诱导分化。 　　结果与结论：成功纯化、扩增了高细胞活性、高分化潜能的骨髓间充质干细胞。所得细胞呈成纤维细胞样；表达CD29、CD90，不表达CD45；成骨、成脂诱导分化后，茜素红染色、油红O染色阳性。证实全骨髓贴壁法操作简单、对细胞活性损伤小，可以得到高纯度、高活性、高分化潜能、生物学形态和特征稳定的骨髓间充质干细胞。
배경：골수래원적간충질간세포함량겁저，체외순화、확증활성호、분화잠능고적골수간충질간세포，대진일보연구지관중요。 　　목적：진일보험증전골수첩벽법체외분리、배양、순화골수간충질간세포적생물학특성、표형급다향분화잠능。 　　방법：통과전골수첩벽법체외분리、배양、순화대서골수간충질간세포，진행형태학관찰，류식세포의검측세포표기물표체，분별진행성골、성지유도분화。 　　결과여결론：성공순화、확증료고세포활성、고분화잠능적골수간충질간세포。소득세포정성섬유세포양；표체CD29、CD90，불표체CD45；성골、성지유도분화후，천소홍염색、유홍O염색양성。증실전골수첩벽법조작간단、대세포활성손상소，가이득도고순도、고활성、고분화잠능、생물학형태화특정은정적골수간충질간세포。
BACKGROUND:Bone marrow mesenchymal stem cells are rare in vivo. It is important to purify, proliferate and differentiate bone marrow mesenchymal stem cells in vitro for further research. OBJECTIVE:To evaluate the biological characteristics, phenotype and multiple differentiation potential cultivation of bone marrow mesenchymal stem cells that are isolated, cultured and purified using the whole bone marrow adherence method. METHODS:Bone marrow mesenchymal stem cells were isolated, purified and cultured by the whole bone marrow adherence method. Morphological observation and flow cytometry determination of cellsurface markers were performed. Osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells was induced. RESULTS AND CONCLUSION:We successful y purified and proliferated bone marrow mesenchymal stem cells with high cellviability and differentiation ability. Fibroblast-like cells were harvested, expressing CD29 and CD90, but not CD45. Fol owing osteogenic and adipogenic induction, cells were positive for oil red O staining and alizarin red staining. The whole bone marrow adherence method is easy to operate, has little impact on cellviability, and can be used to harvest high-purification bone marrow mesenchymal stem cells with high cellviability and differentiation ability.