CAJ | 학술논문

背景：有研究表明椎间盘退变模型的建立可为椎间盘退变治疗提供实验载体，但目前尚缺乏公认的最佳实验动物模型。 　　目的：比较经皮纤维环穿刺法和经肌间隙纤维环刀刺法建立兔椎间盘退变模型的差异。 　　方法：将新西兰大白兔分别采用经皮纤维环穿刺法和经肌间隙纤维环刀刺法建立兔椎间盘退变模型，穿刺后4，8，16周通过磁共振和组织病理学检查观察腰椎间盘髓核变性及组织病理情况。 　　结果与结论：穿刺后4周，两组兔椎间盘髓核内T2加权像信号降低、变暗，椎间隙高度下降，但经皮纤维环穿刺组T2信号强度评分较经肌间隙纤维环刀刺组低(P<0.05)；穿刺后8周，两组T2信号强度评分均增高，经皮纤维环穿刺组T2信号强度评分较经肌间隙纤维环刀刺组低(P<0.05)；穿刺后16周，两组兔椎间盘髓核内T2信号强度评分达最高且差异无显著性意义，两组椎间隙均明显变窄，椎间盘均亮度变黑，两组差异不明显；经皮纤维环穿刺组手术时间少于经肌间隙纤维环刀刺组(P<0.05)，经肌间隙纤维环刀刺组感染率为5.6%，而经皮纤维环穿刺组无感染。结果证实，经皮纤维环穿刺法建立兔椎间盘退变模型建模时间短，感染率低，效果优于经肌间隙纤维环刀刺法造模。
배경：유연구표명추간반퇴변모형적건립가위추간반퇴변치료제공실험재체，단목전상결핍공인적최가실험동물모형。 　　목적：비교경피섬유배천자법화경기간극섬유배도자법건립토추간반퇴변모형적차이。 　　방법：장신서란대백토분별채용경피섬유배천자법화경기간극섬유배도자법건립토추간반퇴변모형，천자후4，8，16주통과자공진화조직병이학검사관찰요추간반수핵변성급조직병리정황。 　　결과여결론：천자후4주，량조토추간반수핵내T2가권상신호강저、변암，추간극고도하강，단경피섬유배천자조T2신호강도평분교경기간극섬유배도자조저(P<0.05)；천자후8주，량조T2신호강도평분균증고，경피섬유배천자조T2신호강도평분교경기간극섬유배도자조저(P<0.05)；천자후16주，량조토추간반수핵내T2신호강도평분체최고차차이무현저성의의，량조추간극균명현변착，추간반균량도변흑，량조차이불명현；경피섬유배천자조수술시간소우경기간극섬유배도자조(P<0.05)，경기간극섬유배도자조감염솔위5.6%，이경피섬유배천자조무감염。결과증실，경피섬유배천자법건립토추간반퇴변모형건모시간단，감염솔저，효과우우경기간극섬유배도자법조모。
BACKGROUND: Studies have shown that the establishment of disc degeneration model provides experimental support for the treatment of intervertebral disc degeneration, but the current study still lacks of recognized optimal animal models. OBJECTIVE: To compare the differences of percutaneous annulus fibrosus needle puncture with muscular annulus fibrosus knife penetration in establishing a rabbit model of intervertebral disc degeneration. METHODS: The New Zealand rabbit models of intervertebral disc degeneration were established by percutaneous annulus fibrosus needle puncture and muscular annulus fibrosus knife penetration. At 4, 8 and 16 weeks after puncture, lumbar disc degeneration and histopathological conditions were observed using magnetic resonance imaging and histopathology examination. RESULTS AND CONCLUSION: At 4 weeks after the puncture, T2-weighted image signal reduced, darken, and disc height decreased in rabbit nucleus pulposus of the two groups. T2 signal intensity scores were lower in the percutaneous annulus fibrosus needle puncture group compared with the muscular annulus fibrosus knife penetration group (P < 0.05). At 8 weeks after puncture, T2 signal intensity scores were increased in both groups. T2 signal intensity scores were lower in the percutaneous annulus fibrosus needle puncture group compared with the muscular annulus fibrosus knife penetration group (P < 0.05). At 16 weeks after puncture, T2 signal intensity reached a peak, and no significant difference was detected between the two groups. Intervertebral space became significantly narrowed. The brightness of the disc became black. There were no significant differences between the two groups. The time of operation was shorter in the percutaneous annulus fibrosus needle puncture group than that in the muscular annulus fibrosus knife penetration group (P < 0.05). Infection rate was 5.6% in the muscular annulus fibrosus knife penetration group. No infection was observed in the percutaneous annulus fibrosus needle puncture group. Results verified that the time of establishing a rabbit model of intervertebral disc degeneration using percutaneous annulus fibrosus needle puncture was short, and the infection rate was low. Thus, its effects were better than muscular annulus fibrosus knife penetration.