CAJ | 학술논문

对0、30、150、300、450和600μmol·L-1 Pb 胁迫条件下狭叶香蒲(Typha angustifolia Linn.)种子的萌发特性进行了研究，并分析了0、450、900、1800和2700μmol·L-1 Pb 胁迫对狭叶香蒲幼苗叶片及根系中部分生理生化指标的影响。结果表明：随 Pb 浓度提高，狭叶香蒲种子的发芽率、发芽势、发芽指数和活力指数以及下胚轴长度均逐渐下降且低于对照，而其下胚轴长度抑制指数则逐渐增大，但在30μmol·L-1 Pb 胁迫条件下各项萌发指标均与对照无显著差异。叶片叶绿素 a、叶绿素 b 及总叶绿素含量随 Pb 浓度提高呈逐渐下降趋势，但在450和900μmol·L-1 Pb 胁迫条件下与对照无显著差异，而在1800和2700μmol·L-1 Pb 胁迫条件下显著低于对照。在 Pb 胁迫条件下叶片和根中 SOD 活性均显著高于对照但变化趋势不同；随 Pb 浓度提高，叶片 SOD 活性呈波动但整体上升的趋势，而根中 SOD 活性则呈逐渐降低的趋势。叶片和根中 POD 活性均随 Pb 浓度提高呈持续上升的趋势，其中，在450和900μmol·L-1 Pb 胁迫条件下叶片的 POD 活性低于对照、根的 POD 活性高于对照，但均与对照无显著差异；而在1800和2700μmol·L-1 Pb 胁迫条件下叶片和根的 POD 活性均显著高于对照。在 Pb 胁迫条件下叶片和根中 AsA和 MDA 含量均高于对照。随 Pb 浓度提高，叶片的 AsA 含量总体上逐渐增加但在450和900μmol·L-1 Pb 胁迫条件下与对照无显著差异；而根的 AsA 含量则呈先增加后降低的趋势且均与对照差异显著。随 Pb 浓度提高，叶片的MDA 含量先增后降但均与对照无显著差异；而根的 MDA 含量呈“高-低-高”的波动趋势且仅在450μmol·L-1 Pb 胁迫条件下与对照差异显著。综合分析结果显示：狭叶香蒲幼苗根系对 Pb 胁迫的敏感性可能强于叶片；狭叶香蒲种子可在轻度 Pb 污染水体中萌发和生长；其幼苗对 Pb 胁迫具有一定的耐性，可用于中度 Pb 污染水体的修复。對0、30、150、300、450和600μmol·L-1 Pb 脅迫條件下狹葉香蒲(Typha angustifolia Linn.)種子的萌髮特性進行瞭研究，併分析瞭0、450、900、1800和2700μmol·L-1 Pb 脅迫對狹葉香蒲幼苗葉片及根繫中部分生理生化指標的影響。結果錶明：隨 Pb 濃度提高，狹葉香蒲種子的髮芽率、髮芽勢、髮芽指數和活力指數以及下胚軸長度均逐漸下降且低于對照，而其下胚軸長度抑製指數則逐漸增大，但在30μmol·L-1 Pb 脅迫條件下各項萌髮指標均與對照無顯著差異。葉片葉綠素 a、葉綠素 b 及總葉綠素含量隨 Pb 濃度提高呈逐漸下降趨勢，但在450和900μmol·L-1 Pb 脅迫條件下與對照無顯著差異，而在1800和2700μmol·L-1 Pb 脅迫條件下顯著低于對照。在 Pb 脅迫條件下葉片和根中 SOD 活性均顯著高于對照但變化趨勢不同；隨 Pb 濃度提高，葉片 SOD 活性呈波動但整體上升的趨勢，而根中 SOD 活性則呈逐漸降低的趨勢。葉片和根中 POD 活性均隨 Pb 濃度提高呈持續上升的趨勢，其中，在450和900μmol·L-1 Pb 脅迫條件下葉片的 POD 活性低于對照、根的 POD 活性高于對照，但均與對照無顯著差異；而在1800和2700μmol·L-1 Pb 脅迫條件下葉片和根的 POD 活性均顯著高于對照。在 Pb 脅迫條件下葉片和根中 AsA和 MDA 含量均高于對照。隨 Pb 濃度提高，葉片的 AsA 含量總體上逐漸增加但在450和900μmol·L-1 Pb 脅迫條件下與對照無顯著差異；而根的 AsA 含量則呈先增加後降低的趨勢且均與對照差異顯著。隨 Pb 濃度提高，葉片的MDA 含量先增後降但均與對照無顯著差異；而根的 MDA 含量呈“高-低-高”的波動趨勢且僅在450μmol·L-1 Pb 脅迫條件下與對照差異顯著。綜閤分析結果顯示：狹葉香蒲幼苗根繫對 Pb 脅迫的敏感性可能彊于葉片；狹葉香蒲種子可在輕度 Pb 汙染水體中萌髮和生長；其幼苗對 Pb 脅迫具有一定的耐性，可用于中度 Pb 汙染水體的脩複。
대0、30、150、300、450화600μmol·L-1 Pb 협박조건하협협향포(Typha angustifolia Linn.)충자적맹발특성진행료연구，병분석료0、450、900、1800화2700μmol·L-1 Pb 협박대협협향포유묘협편급근계중부분생리생화지표적영향。결과표명：수 Pb 농도제고，협협향포충자적발아솔、발아세、발아지수화활력지수이급하배축장도균축점하강차저우대조，이기하배축장도억제지수칙축점증대，단재30μmol·L-1 Pb 협박조건하각항맹발지표균여대조무현저차이。협편협록소 a、협록소 b 급총협록소함량수 Pb 농도제고정축점하강추세，단재450화900μmol·L-1 Pb 협박조건하여대조무현저차이，이재1800화2700μmol·L-1 Pb 협박조건하현저저우대조。재 Pb 협박조건하협편화근중 SOD 활성균현저고우대조단변화추세불동；수 Pb 농도제고，협편 SOD 활성정파동단정체상승적추세，이근중 SOD 활성칙정축점강저적추세。협편화근중 POD 활성균수 Pb 농도제고정지속상승적추세，기중，재450화900μmol·L-1 Pb 협박조건하협편적 POD 활성저우대조、근적 POD 활성고우대조，단균여대조무현저차이；이재1800화2700μmol·L-1 Pb 협박조건하협편화근적 POD 활성균현저고우대조。재 Pb 협박조건하협편화근중 AsA화 MDA 함량균고우대조。수 Pb 농도제고，협편적 AsA 함량총체상축점증가단재450화900μmol·L-1 Pb 협박조건하여대조무현저차이；이근적 AsA 함량칙정선증가후강저적추세차균여대조차이현저。수 Pb 농도제고，협편적MDA 함량선증후강단균여대조무현저차이；이근적 MDA 함량정“고-저-고”적파동추세차부재450μmol·L-1 Pb 협박조건하여대조차이현저。종합분석결과현시：협협향포유묘근계대 Pb 협박적민감성가능강우협편；협협향포충자가재경도 Pb 오염수체중맹발화생장；기유묘대 Pb 협박구유일정적내성，가용우중도 Pb 오염수체적수복。
Seed germination characteristics of Typha angustifolia Linn. under Pb stress with concentrations of 0, 30, 150, 300, 450 and 600 μmol·L-1 were studied, and effects of Pb stress with concentrations of 0, 450, 900, 1 800 and 2 700 μmol·L-1 on some physiological and biochemical indexes in leaf and root of seedling were analyzed. The results show that with rising of Pb concentration, germination rate, germination energy, germination index and vigor index and hypocotyl length of T.angustifolia seed all decrease gradually and are lower than those of the control, while inhibition index of hypocotyl length increases gradually, but under 30 μmol·L-1 Pb stress, there is no significant difference in each germination index with the control. Contents of chla, chlb and total chlorophyll in leaf appear the trend of decreasing gradually with rising of Pb concentration, but those under 450 and 900 μmol·L-1 Pb stress have no significant differences comparing with those of the control, while those under 1 800 and 2 700 μmol·L-1 Pb stress are significantly lower than those of the control. Under Pb stress, SOD activity in leaf and root is significantly higher than that of the control but their variation trend is different. With rising of Pb concentration, SOD activity in leaf appears a fluctuant but totally increasing trend, while that in root appears a gradually decreasing trend. POD activity in leaf and root appears a continually increasing trend with rising of Pb concentration. In which, under 450 and 900 μmol·L-1 Pb stress, POD activity in leaf is lower and that in root is higher than that of the control but with no significant difference to the control, while POD activity in leaf and root under 1 800 and 2 700 μmol·L-1 Pb stress is significantly higher than that of the control. Under Pb stress, contents of AsA and MDA in leaf and root are higher than those of the control. With rising of Pb concentration, overall, AsA content in leaf increases gradually but that under 450 and 900 μmol · L-1 Pb stress has no significant difference comparing with the control, while AsA content in root appears the trend of firstly increasing and then decreasing with a significant difference to the control. With rising of Pb concentration, MDA content in leaf firstly increases and then decreases but with no significant difference to the control, while MDA content in root appears the fluctuant trend of “high-low-high” and only that under 450 μmol·L-1 Pb stress has a significant difference comparing with the control. The comprehensive analysis result indicates that the sensitiveness of root of T. angustifolia to Pb stress is probably stronger than that of leaf. And T.angustifolia seed can germinate and grow in light Pb polluted water and its seedling has a certain tolerance to Pb stress, so it can be used for repairing middle Pb polluted water.