植物资源与环境学报
植物資源與環境學報
식물자원여배경학보
JOURNAL OF PLANT RESOURCES AND ENVIRONMENT
2014年
3期
65-73
,共9页
杨淑红%宋德才%刘艳萍%杨清淮%朱延林
楊淑紅%宋德纔%劉豔萍%楊清淮%硃延林
양숙홍%송덕재%류염평%양청회%주연림
杨树品种%干旱胁迫%保护酶活性%抗旱性%隶属函数法%灰色关联分析
楊樹品種%榦旱脅迫%保護酶活性%抗旱性%隸屬函數法%灰色關聯分析
양수품충%간한협박%보호매활성%항한성%대속함수법%회색관련분석
cultivar of Populus%drought stress%protective enzyme activity%drought resistance%subordinate function method%grey relational analysis
以美洲黑杨品种‘2025’( Populus × deltoides ‘2025’)及其2个芽变彩叶品种‘全红杨’( P.× deltoides‘Quanhong’)和‘中红杨’(P.× deltoides ‘Zhonghong’)当年生嫁接苗为实验材料,对土壤干旱胁迫0、4、7、14、21和28 d 以及复水7 d 后叶片中 MDA 含量及 PPO、SOD、CAT 和 POD 活性的变化以及 MDA 含量与4种酶活性的相关性进行了分析,并采用隶属函数法和灰色关联分析法对3个品种的抗旱性进行综合评价。结果表明：随干旱胁迫时间的延长,3个品种的 MDA 含量总体呈增加趋势,并在处理的中期和后期显著或极显著高于各自的对照；PPO 和SOD 活性总体上先升高后降低,均在处理的前期和中期高于各自的对照；‘中红杨’和‘2025’的 CAT 活性先升高后下降,而‘全红杨’的 CAT 活性持续下降,均在处理14 d 后极显著或显著低于各自的对照；‘全红杨’和‘2025’的POD 活性先降低后升高且均低于各自的对照,而‘中红杨’的 POD 活性则呈波动趋势但总体上与对照无显著差异。复水7 d 后,3个品种的 MDA 含量均有所降低,而 PPO 和 CAT 活性均有所提高；‘全红杨’和‘2025’的 SOD 和 POD活性升高,而‘中红杨’的 SOD 和 POD 活性则降低。在土壤干旱胁迫条件下,仅‘全红杨’的 PPO 和 CAT 活性与MDA 含量以及‘中红杨’的 CAT 活性与 MDA 含量呈极显著负相关,各品种的其他酶活性与 MDA 含量均无显著相关性。‘全红杨’、‘中红杨’和‘2025’的平均隶属函数值分别为0.482、0.527和0.533,据此判断3个品种中‘2025’的抗旱性最强、‘全红杨’的抗旱性最弱。3个品种抗旱性与各生理指标的关联度均大于0.64,其中与 CAT和 PPO 活性的关联度分别为0.793和0.761,因而,可将 CAT 和 PPO 活性作为评价杨树品种抗旱性的首选指标。
以美洲黑楊品種‘2025’( Populus × deltoides ‘2025’)及其2箇芽變綵葉品種‘全紅楊’( P.× deltoides‘Quanhong’)和‘中紅楊’(P.× deltoides ‘Zhonghong’)噹年生嫁接苗為實驗材料,對土壤榦旱脅迫0、4、7、14、21和28 d 以及複水7 d 後葉片中 MDA 含量及 PPO、SOD、CAT 和 POD 活性的變化以及 MDA 含量與4種酶活性的相關性進行瞭分析,併採用隸屬函數法和灰色關聯分析法對3箇品種的抗旱性進行綜閤評價。結果錶明：隨榦旱脅迫時間的延長,3箇品種的 MDA 含量總體呈增加趨勢,併在處理的中期和後期顯著或極顯著高于各自的對照；PPO 和SOD 活性總體上先升高後降低,均在處理的前期和中期高于各自的對照；‘中紅楊’和‘2025’的 CAT 活性先升高後下降,而‘全紅楊’的 CAT 活性持續下降,均在處理14 d 後極顯著或顯著低于各自的對照；‘全紅楊’和‘2025’的POD 活性先降低後升高且均低于各自的對照,而‘中紅楊’的 POD 活性則呈波動趨勢但總體上與對照無顯著差異。複水7 d 後,3箇品種的 MDA 含量均有所降低,而 PPO 和 CAT 活性均有所提高；‘全紅楊’和‘2025’的 SOD 和 POD活性升高,而‘中紅楊’的 SOD 和 POD 活性則降低。在土壤榦旱脅迫條件下,僅‘全紅楊’的 PPO 和 CAT 活性與MDA 含量以及‘中紅楊’的 CAT 活性與 MDA 含量呈極顯著負相關,各品種的其他酶活性與 MDA 含量均無顯著相關性。‘全紅楊’、‘中紅楊’和‘2025’的平均隸屬函數值分彆為0.482、0.527和0.533,據此判斷3箇品種中‘2025’的抗旱性最彊、‘全紅楊’的抗旱性最弱。3箇品種抗旱性與各生理指標的關聯度均大于0.64,其中與 CAT和 PPO 活性的關聯度分彆為0.793和0.761,因而,可將 CAT 和 PPO 活性作為評價楊樹品種抗旱性的首選指標。
이미주흑양품충‘2025’( Populus × deltoides ‘2025’)급기2개아변채협품충‘전홍양’( P.× deltoides‘Quanhong’)화‘중홍양’(P.× deltoides ‘Zhonghong’)당년생가접묘위실험재료,대토양간한협박0、4、7、14、21화28 d 이급복수7 d 후협편중 MDA 함량급 PPO、SOD、CAT 화 POD 활성적변화이급 MDA 함량여4충매활성적상관성진행료분석,병채용대속함수법화회색관련분석법대3개품충적항한성진행종합평개。결과표명：수간한협박시간적연장,3개품충적 MDA 함량총체정증가추세,병재처리적중기화후기현저혹겁현저고우각자적대조；PPO 화SOD 활성총체상선승고후강저,균재처리적전기화중기고우각자적대조；‘중홍양’화‘2025’적 CAT 활성선승고후하강,이‘전홍양’적 CAT 활성지속하강,균재처리14 d 후겁현저혹현저저우각자적대조；‘전홍양’화‘2025’적POD 활성선강저후승고차균저우각자적대조,이‘중홍양’적 POD 활성칙정파동추세단총체상여대조무현저차이。복수7 d 후,3개품충적 MDA 함량균유소강저,이 PPO 화 CAT 활성균유소제고；‘전홍양’화‘2025’적 SOD 화 POD활성승고,이‘중홍양’적 SOD 화 POD 활성칙강저。재토양간한협박조건하,부‘전홍양’적 PPO 화 CAT 활성여MDA 함량이급‘중홍양’적 CAT 활성여 MDA 함량정겁현저부상관,각품충적기타매활성여 MDA 함량균무현저상관성。‘전홍양’、‘중홍양’화‘2025’적평균대속함수치분별위0.482、0.527화0.533,거차판단3개품충중‘2025’적항한성최강、‘전홍양’적항한성최약。3개품충항한성여각생리지표적관련도균대우0.64,기중여 CAT화 PPO 활성적관련도분별위0.793화0.761,인이,가장 CAT 화 PPO 활성작위평개양수품충항한성적수선지표。
Taking annual grafted seedlings of Populus í deltoides ‘2025’ and its two bud mutation color-leaf cultivars of P. í deltoides ‘Quanhong’ and P. í deltoides ‘Zhonghong’ as experimental materials, changes of MDA content and activities of PPO, SOD, CAT and POD in their leaf after drought stress for 0, 4, 7, 14, 21 and 28 d and rewatering for 7 d and correlations between MDA content and four enzyme activities were analyzed, and drought resistance of three cultivars was comprehensively evaluated by subordinate function method and grey relational analysis method. The results show that with prolonging of drought stress time, MDA content of three cultivars appears a generally increasing trend and is significantly and extremely significantly higher than that of their respective controls at the middle and later treatment stages. On the whole, PPO and SOD activities firstly increase and then decrease and are higher than those of their respective controls at the earlier and middle treatment stages. CAT activity of ‘Zhonghong’ and ‘2025’ firstly increases and then decreases, while that of ‘ Quanhong’ decreases continuously, and is extremely significantly and significantly lower than that of their respective controls after treated for 14 d. POD activity of ‘Quanhong’ and ‘2025’ firstly decreases and then increases and is lower than that of their respective controls, while that of ‘Zhonghong’ appears a fluctuant trend but overall with no significant difference in comparison with that of its control. After rewatering for 7 d, MDA content of three cultivars all decreases, while PPO and CAT activities all increase. SOD and POD activities of ‘ Quanhong’ and ‘2025’ increase, while those of ‘ Zhonghong’ decrease. Under soil drought stress condition, there are only extremely significantly negative correlations between PPO and CAT activities and MDA content in ‘ Quanhong ’ and between CAT activity and MDA content in‘Zhonghong’, there is no significant correlation between other enzyme activities and MDA content in all cultivars. Subordinate function average value of ‘Quanhong’, ‘Zhonghong’ and ‘2025’ is 0. 482, 0. 527 and 0. 533, respectively. Accordingly, among three cultivars, drought resistance of ‘2025’ is the strongest, while that of ‘ Quanhong’ is the weakest. Correlation degree of drought resistance of three cultivars with physiological indexes all is higher than 0. 64, in which, that of drought resistance with CAT and PPO activities is 0. 793 and 0. 761, respectively. Therefore, CAT and PPO activities can be regarded as main indexes for evaluating drought resistance of cultivars of Populus.
