植物资源与环境学报
植物資源與環境學報
식물자원여배경학보
JOURNAL OF PLANT RESOURCES AND ENVIRONMENT
2014年
3期
59-64
,共6页
香根草%培养液pH值%植株形态%生理特性%酸碱适应性
香根草%培養液pH值%植株形態%生理特性%痠堿適應性
향근초%배양액pH치%식주형태%생리특성%산감괄응성
Vetiveria zizanioides (Linn.) Nash%pH value of solution%plant morphology%physiological characteristics%acidity-alkalinity adaptation
在 pH 2.0~ pH 11.0的1/2 Hoagland 培养液水培条件下,对培养20 d 后香根草也Vetiveria zizanioides (Linn.) Nash页幼苗形态、叶片和根系部分生理指标的变化进行了研究。结果表明:在强碱性(pH 11.0和 pH 10.0)及强酸性(pH 2.0和 pH 3.0)条件下,香根草幼苗的部分叶片枯黄,茎干枯,须根数量明显减少且长度趋短;而在弱碱性(pH 9.0和 pH 8.0)及弱酸性(pH 5.0和 pH 4.0)条件下,仅可见部分叶尖出现轻度失绿现象,根系形态无明显变化仅颜色有差异。随培养液 pH 值的增大(碱性增加)或减小(酸性增加),叶片含水量和叶绿素含量均逐渐降低且均小于对照(pH 6.5),而相对电解质外渗率和 MDA 含量均逐渐增加并高于对照;各指标在强碱性(pH 11.0和pH 10.0)及强酸性(pH 2.0和 pH 3.0)条件下总体上与对照差异显著(P<0.05),而在弱碱性(pH 9.0和 pH 8.0)及弱酸性(pH 5.0和 pH 4.0)条件下总体上与对照差异不显著(P>0.05)。随培养液 pH 值的增大(碱性增加)或减小(酸性增加),幼苗的最大根长和根系活力均逐渐降低;在弱碱性(pH 8.0)和弱酸性(pH 5.0)条件下,最大根长略小于对照、根系活力略大于对照,但差异均不显著;而在 pH 9.0~ pH 11.0及 pH 4.0~ pH 2.0条件下最大根长及根系活力均显著小于对照,其中,在 pH 11.0条件下根系最短、根系活力最小,分别仅为对照的60.50%和9.52%。研究结果揭示:香根草对环境酸碱性的适应范围较广,可适应 pH 4.0~ pH 9.0的土壤生境。
在 pH 2.0~ pH 11.0的1/2 Hoagland 培養液水培條件下,對培養20 d 後香根草也Vetiveria zizanioides (Linn.) Nash頁幼苗形態、葉片和根繫部分生理指標的變化進行瞭研究。結果錶明:在彊堿性(pH 11.0和 pH 10.0)及彊痠性(pH 2.0和 pH 3.0)條件下,香根草幼苗的部分葉片枯黃,莖榦枯,鬚根數量明顯減少且長度趨短;而在弱堿性(pH 9.0和 pH 8.0)及弱痠性(pH 5.0和 pH 4.0)條件下,僅可見部分葉尖齣現輕度失綠現象,根繫形態無明顯變化僅顏色有差異。隨培養液 pH 值的增大(堿性增加)或減小(痠性增加),葉片含水量和葉綠素含量均逐漸降低且均小于對照(pH 6.5),而相對電解質外滲率和 MDA 含量均逐漸增加併高于對照;各指標在彊堿性(pH 11.0和pH 10.0)及彊痠性(pH 2.0和 pH 3.0)條件下總體上與對照差異顯著(P<0.05),而在弱堿性(pH 9.0和 pH 8.0)及弱痠性(pH 5.0和 pH 4.0)條件下總體上與對照差異不顯著(P>0.05)。隨培養液 pH 值的增大(堿性增加)或減小(痠性增加),幼苗的最大根長和根繫活力均逐漸降低;在弱堿性(pH 8.0)和弱痠性(pH 5.0)條件下,最大根長略小于對照、根繫活力略大于對照,但差異均不顯著;而在 pH 9.0~ pH 11.0及 pH 4.0~ pH 2.0條件下最大根長及根繫活力均顯著小于對照,其中,在 pH 11.0條件下根繫最短、根繫活力最小,分彆僅為對照的60.50%和9.52%。研究結果揭示:香根草對環境痠堿性的適應範圍較廣,可適應 pH 4.0~ pH 9.0的土壤生境。
재 pH 2.0~ pH 11.0적1/2 Hoagland 배양액수배조건하,대배양20 d 후향근초야Vetiveria zizanioides (Linn.) Nash혈유묘형태、협편화근계부분생리지표적변화진행료연구。결과표명:재강감성(pH 11.0화 pH 10.0)급강산성(pH 2.0화 pH 3.0)조건하,향근초유묘적부분협편고황,경간고,수근수량명현감소차장도추단;이재약감성(pH 9.0화 pH 8.0)급약산성(pH 5.0화 pH 4.0)조건하,부가견부분협첨출현경도실록현상,근계형태무명현변화부안색유차이。수배양액 pH 치적증대(감성증가)혹감소(산성증가),협편함수량화협록소함량균축점강저차균소우대조(pH 6.5),이상대전해질외삼솔화 MDA 함량균축점증가병고우대조;각지표재강감성(pH 11.0화pH 10.0)급강산성(pH 2.0화 pH 3.0)조건하총체상여대조차이현저(P<0.05),이재약감성(pH 9.0화 pH 8.0)급약산성(pH 5.0화 pH 4.0)조건하총체상여대조차이불현저(P>0.05)。수배양액 pH 치적증대(감성증가)혹감소(산성증가),유묘적최대근장화근계활력균축점강저;재약감성(pH 8.0)화약산성(pH 5.0)조건하,최대근장략소우대조、근계활력략대우대조,단차이균불현저;이재 pH 9.0~ pH 11.0급 pH 4.0~ pH 2.0조건하최대근장급근계활력균현저소우대조,기중,재 pH 11.0조건하근계최단、근계활력최소,분별부위대조적60.50%화9.52%。연구결과게시:향근초대배경산감성적괄응범위교엄,가괄응 pH 4.0~ pH 9.0적토양생경。
Under hydroponic culture with 1 / 2 Hoagland solution of pH 2. 0-pH 11. 0, seedling morphology, changes of some physiological indexes of leaf and root of Vetiveria zizanioides (Linn.) Nash were researched after cultured for 20 d. The results show that V. zizanioides seedling appears a part of leaves firing, stem withering, fibrous root number decreasing obviously and its length shortening under conditions of strong alkalinity (pH 11. 0 and pH 10. 0) and strong acidity (pH 2. 0 and pH 3. 0), and the seedling only appears a part of leaf apexes with a little chlorosis and root morphology without obvious change except color under conditions of weak alkalinity ( pH 9. 0 and pH 8. 0) and weak acidity (pH 5. 0 and pH 4. 0). With pH value of solution increasing ( alkalinity increasing) or decreasing (acidity increasing), both leaf water content and chlorophyll content decrease gradually and are lower than those of the control (pH 6. 5), while both relative electrolytic leakage and MDA content increase gradually and are higher than those of the control. In general, there are significant (P<0. 05) differences in all indexes under conditions of strong alkalinity (pH 11. 0 and pH 10. 0) and strong acidity (pH 2. 0 and pH 3. 0) with the control, while generally without significant (P>0. 05) difference in all indexes under conditions of weak alkalinity (pH 9. 0 and pH 8. 0) and weak acidity (pH 5. 0 and pH 4. 0) with the control. With pH value of solution increasing ( alkalinity increasing ) or decreasing ( acidity increasing), both the maximum root length and root activity decrease gradually. Under conditions of weak alkalinity (pH 8. 0) and weak acidity (pH 5. 0), the maximum root length is slightly lower than that of the control and root activity is slightly higher than that of the control without significant difference. Under pH 9. 0-pH 11. 0 and pH 4. 0-pH 2. 0, both the maximum root length and root activity are significantly lower than those of the control, in which root length is the shortest and root activity is the smallest under pH 11. 0, with 60. 50% and 9. 52% of the control,respectively. It is suggested that V. zizanioides has a wide adaptation range to acidity-alkalinity of soil, and can adapt soil habitat with pH 4. 0-pH 9. 0.