CAJ | 학술논문

以茶树也Camellia sinensis (Linn.) Kuntze页品种‘舒茶早’(‘Shuchazao’)、‘乌牛早’(‘Wuniuzao’)和‘平阳特早’(‘Pingyangtezao’)为实验材料，分析了自然越冬过程中(2011年10月至2012年3月)茶树叶片主要渗透调节物质(可溶性糖、可溶性蛋白质、热稳定蛋白质和脯氨酸)含量、抗氧化酶(SOD、CAT 和 POD)活性、PSⅡ原初光能转化效率(Fv / Fm )和总叶绿素含量的变化，并应用主成分分析结合隶属函数法对3个茶树品种抗寒性进行综合评价。结果表明：自然越冬期3个茶树品种叶片生理指标随时间推移呈规律性变化，越冬期(12月和1月)与越冬前期(10月)、越冬后期(2月和3月)各指标总体上差异显著，且品种间各指标也有一定差异。可溶性糖、可溶性蛋白质、热稳定蛋白质和脯氨酸含量以及 SOD 活性均呈先升后降的变化趋势，且峰值多出现在12月或2月；CAT 和POD 活性、Fv / Fm值和总叶绿素含量则呈先降后升的变化趋势，且均在1月降至最低。主成分分析结果显示：第1主成分(X1)包括可溶性糖含量、SOD 活性、CAT 活性、POD 活性、Fv / Fm和总叶绿素含量，贡献率达到57.97%；第2主成分(X2)包括可溶性蛋白质含量、热稳定蛋白质含量和脯氨酸含量，贡献率达到26.89%。综合评价结果显示：自然越冬期3个茶树品种抗寒性的综合评价值均随时间推移呈先升后降的变化趋势，且均在12月达到最高，据此判断3个茶树品种中‘舒茶早’的抗寒性最强、‘乌牛早’的抗寒性最弱，‘平阳特早’的抗寒性介于二者之间。研究结果显示：在自然越冬过程中供试3个茶树品种的抗寒性与其生理指标变化及生长表现一致，表明综合评价法可用于茶树抗寒性评价。
이다수야Camellia sinensis (Linn.) Kuntze혈품충‘서다조’(‘Shuchazao’)、‘오우조’(‘Wuniuzao’)화‘평양특조’(‘Pingyangtezao’)위실험재료，분석료자연월동과정중(2011년10월지2012년3월)다수협편주요삼투조절물질(가용성당、가용성단백질、열은정단백질화포안산)함량、항양화매(SOD、CAT 화 POD)활성、PSⅡ원초광능전화효솔(Fv / Fm )화총협록소함량적변화，병응용주성분분석결합대속함수법대3개다수품충항한성진행종합평개。결과표명：자연월동기3개다수품충협편생리지표수시간추이정규률성변화，월동기(12월화1월)여월동전기(10월)、월동후기(2월화3월)각지표총체상차이현저，차품충간각지표야유일정차이。가용성당、가용성단백질、열은정단백질화포안산함량이급 SOD 활성균정선승후강적변화추세，차봉치다출현재12월혹2월；CAT 화POD 활성、Fv / Fm치화총협록소함량칙정선강후승적변화추세，차균재1월강지최저。주성분분석결과현시：제1주성분(X1)포괄가용성당함량、SOD 활성、CAT 활성、POD 활성、Fv / Fm화총협록소함량，공헌솔체도57.97%；제2주성분(X2)포괄가용성단백질함량、열은정단백질함량화포안산함량，공헌솔체도26.89%。종합평개결과현시：자연월동기3개다수품충항한성적종합평개치균수시간추이정선승후강적변화추세，차균재12월체도최고，거차판단3개다수품충중‘서다조’적항한성최강、‘오우조’적항한성최약，‘평양특조’적항한성개우이자지간。연구결과현시：재자연월동과정중공시3개다수품충적항한성여기생리지표변화급생장표현일치，표명종합평개법가용우다수항한성평개。
Taking cultivars ‘Shuchazao’, ‘Wuniuzao’and ‘Pingyangtezao’of Camellia sinensis (Linn.) Kuntze as experimental materials, changes in main osmoregulation substance ( soluble sugar, soluble protein, heat-stable protein and proline) content, antioxidant enzyme (SOD, CAT and POD) activity, the maximum quantum efficiency of PSII(Fv / Fm ) and total chlorophyll content in leaf of C. sinensis during natural overwintering period (from Oct. 2011 to Mar. 2012) were researched, and cold resistance of three cultivars of C. sinensis was comprehensively evaluated by principal component analysis combined with subordinate function method. The results show that during natural overwintering period, there is a regular change in physiological indexes in leaf of three cultivars with the passage of time, and difference in each index among overwintering stage (Dec. and Jan.), pre-winter stage (Oct.) and post-winter stage (Feb. and Mar.) is generally significant and there is a certain difference in each index among different cultivars. Contents of soluble sugar, soluble protein, heat-stable protein and proline and SOD activity increase firstly and then decrease, and the peak value mostly appears at Dec. or Feb. While CAT and POD activities, Fv / Fm value and total chlorophyll content decrease firstly and then increase, and all appear the minimum at Jan. The result of principal component analysis shows that the first principal component (X1) includes soluble sugar content, activities of SOD, CAT and POD, Fv / Fm and total chlorophyll content with the contribution rate of 57. 97% , and the second principal component ( X2) includes contents of soluble protein, heat-stable protein and proline with contribution rate of 26. 89% . The result of comprehensive evaluation shows that during natural overwintering period, the comprehensive evaluation value of cold resistance of three cultivars all increases firstly and then decreases with the passage of time, and all reach the maximum at Dec. According to this evaluation, cold resistance of cultivar ‘Shuchazao’ is the strongest in three cultivars, that of cultivar ‘Wuniuzao’ is the weakest, and that of cultivar ‘ Pingyangtezao’ is between them. It is suggested that during natural overwintering period, cold resistance of three cultivars tested is consistent with physiological index change and growth appearance, as a result, comprehensive evaluation method can be used to evaluate cold resistance of C.sinensis.