植物资源与环境学报
植物資源與環境學報
식물자원여배경학보
JOURNAL OF PLANT RESOURCES AND ENVIRONMENT
2014年
3期
36-44
,共9页
麻栎%地理种源%表型性状%变异系数%相对极差%聚类分析
痳櫟%地理種源%錶型性狀%變異繫數%相對極差%聚類分析
마력%지리충원%표형성상%변이계수%상대겁차%취류분석
Quercus acutissima Linn.%geographical provenance%phenotypic trait%coefficient of variation%relative range%cluster analysis
以来源于广西、广东、贵州、湖南、浙江、安徽、江苏、湖北、四川、陕西、河南和山东的28个麻栎(Quercus acutissima Linn.)地理种源为研究对象,分析了6个表型性状(包括叶长、叶宽、叶柄长和叶长宽比4个叶片性状及株高和地径2个生长性状)的变异状况;研究了种源间及种源内各性状的变异系数(CV)和相对极差(R°)及种源间各叶片性状的表型分化系数(Vst);并进行了各性状的相关性分析及主成分分析;此外,还对28个种源进行了聚类分析。结果表明:各种源间6个表型性状均有极显著差异(P<0.01);而种源内仅叶宽和叶长分别具有极显著和显著(P<0.05)差异。种源间4个叶片性状的 Vst 平均值为23.98%,其中叶宽的 Vst 值最大(42.39%)。在6个表型性状中,叶柄长的 CV 平均值最大(33.60%)、地径的 CV 平均值最小(16.25%);在不同种源中,陕西汉中种源各性状的 CV 平均值最小(14.39%),而江苏句容、四川万源和山东沂水种源各性状的 CV 平均值均超过30%。各种源间表型性状的 R°值差异明显,其中,四川万源种源的 R°值最大(60.99%),湖南新宁和陕西汉中种源的 R°值较小(30%以下);而6个性状间 R°值差别不大,为34.93%~44.98%,表明麻栎种源间的极端差异程度有明显不同,而表型性状间的极端差异程度差异不大。4个叶片性状中,仅叶宽与叶长宽比呈极显著负相关,其他性状间均呈极显著正相关;叶片性状与生长性状间均呈负相关,而株高与地径则呈极显著正相关。麻栎种源表型性状变异的第1和第2主成分的贡献率分别为43.663%和27.695%,第1主成分中叶性状变异占主导地位,而第2主成分则主要反映生长性状的变异。通过聚类分析可将28个种源分为2大类,分别包含22和6个种源,但聚类分析结果与其地理分布没有明显相关性。研究结果显示:麻栎种源具有丰富的遗传变异,且其表型性状变异主要存在于种源内。
以來源于廣西、廣東、貴州、湖南、浙江、安徽、江囌、湖北、四川、陝西、河南和山東的28箇痳櫟(Quercus acutissima Linn.)地理種源為研究對象,分析瞭6箇錶型性狀(包括葉長、葉寬、葉柄長和葉長寬比4箇葉片性狀及株高和地徑2箇生長性狀)的變異狀況;研究瞭種源間及種源內各性狀的變異繫數(CV)和相對極差(R°)及種源間各葉片性狀的錶型分化繫數(Vst);併進行瞭各性狀的相關性分析及主成分分析;此外,還對28箇種源進行瞭聚類分析。結果錶明:各種源間6箇錶型性狀均有極顯著差異(P<0.01);而種源內僅葉寬和葉長分彆具有極顯著和顯著(P<0.05)差異。種源間4箇葉片性狀的 Vst 平均值為23.98%,其中葉寬的 Vst 值最大(42.39%)。在6箇錶型性狀中,葉柄長的 CV 平均值最大(33.60%)、地徑的 CV 平均值最小(16.25%);在不同種源中,陝西漢中種源各性狀的 CV 平均值最小(14.39%),而江囌句容、四川萬源和山東沂水種源各性狀的 CV 平均值均超過30%。各種源間錶型性狀的 R°值差異明顯,其中,四川萬源種源的 R°值最大(60.99%),湖南新寧和陝西漢中種源的 R°值較小(30%以下);而6箇性狀間 R°值差彆不大,為34.93%~44.98%,錶明痳櫟種源間的極耑差異程度有明顯不同,而錶型性狀間的極耑差異程度差異不大。4箇葉片性狀中,僅葉寬與葉長寬比呈極顯著負相關,其他性狀間均呈極顯著正相關;葉片性狀與生長性狀間均呈負相關,而株高與地徑則呈極顯著正相關。痳櫟種源錶型性狀變異的第1和第2主成分的貢獻率分彆為43.663%和27.695%,第1主成分中葉性狀變異佔主導地位,而第2主成分則主要反映生長性狀的變異。通過聚類分析可將28箇種源分為2大類,分彆包含22和6箇種源,但聚類分析結果與其地理分佈沒有明顯相關性。研究結果顯示:痳櫟種源具有豐富的遺傳變異,且其錶型性狀變異主要存在于種源內。
이래원우엄서、엄동、귀주、호남、절강、안휘、강소、호북、사천、협서、하남화산동적28개마력(Quercus acutissima Linn.)지리충원위연구대상,분석료6개표형성상(포괄협장、협관、협병장화협장관비4개협편성상급주고화지경2개생장성상)적변이상황;연구료충원간급충원내각성상적변이계수(CV)화상대겁차(R°)급충원간각협편성상적표형분화계수(Vst);병진행료각성상적상관성분석급주성분분석;차외,환대28개충원진행료취류분석。결과표명:각충원간6개표형성상균유겁현저차이(P<0.01);이충원내부협관화협장분별구유겁현저화현저(P<0.05)차이。충원간4개협편성상적 Vst 평균치위23.98%,기중협관적 Vst 치최대(42.39%)。재6개표형성상중,협병장적 CV 평균치최대(33.60%)、지경적 CV 평균치최소(16.25%);재불동충원중,협서한중충원각성상적 CV 평균치최소(14.39%),이강소구용、사천만원화산동기수충원각성상적 CV 평균치균초과30%。각충원간표형성상적 R°치차이명현,기중,사천만원충원적 R°치최대(60.99%),호남신저화협서한중충원적 R°치교소(30%이하);이6개성상간 R°치차별불대,위34.93%~44.98%,표명마력충원간적겁단차이정도유명현불동,이표형성상간적겁단차이정도차이불대。4개협편성상중,부협관여협장관비정겁현저부상관,기타성상간균정겁현저정상관;협편성상여생장성상간균정부상관,이주고여지경칙정겁현저정상관。마력충원표형성상변이적제1화제2주성분적공헌솔분별위43.663%화27.695%,제1주성분중협성상변이점주도지위,이제2주성분칙주요반영생장성상적변이。통과취류분석가장28개충원분위2대류,분별포함22화6개충원,단취류분석결과여기지리분포몰유명현상관성。연구결과현시:마력충원구유봉부적유전변이,차기표형성상변이주요존재우충원내。
Taking twenty-eight geographical provenances of Quercus acutissima Linn. from Guangxi, Guangdong, Guizhou, Hu’nan, Zhejiang, Anhui, Jiangsu, Hubei, Sichuan, Shaanxi, He’nan and Shandong as research objects, variation status of six phenotypic traits including four leaf traits ( leaf length, leaf width, petiole length and ratio of leaf length to leaf width) and two growth traits (height and ground diameter) was analyzed. Coefficient of variation (CV) and relative range (R°) of all traits among and within provenances and phenotypic differentiation coefficient ( Vst ) of all leaf traits among provenances were studied, and analyses on correlation and principal component of all traits were also carried out. Moreover, the cluster analysis on twenty-eight provenances was also done. Results show that there are extremely significant ( P < 0. 01 ) differences in six phenotypic traits among different provenances, while within provenances, there are extremely significant and significant ( P < 0. 05 ) differences only in leaf width and leaf length, respectively. Average value of Vst of four leaf traits among provenances is 23. 98% , in which Vst value of leaf width is the biggest (42. 39% ). In six phenotypic traits, CV average value of petiole length is the biggest (33. 60% ) and that of ground diameter is the smallest (16. 25% ), and among different provenances, CV average value of all traits of provenance from Hanzhong of Shaanxi is the smallest (14. 39% ), while that from Jurong of Jiangsu, Wanyuan of Sichuan and Yishui of Shandong all is above 30% . There are obvious difference in R° value of all traits among provenances, in which, R° value of provenance from Wanyuan of Sichuan is the biggest (60. 99%), and that from Xinning of Hu’nan and Hanzhong of Shaanxi is smaller (less than 30% ), while that among six phenotypic traits is 34. 93% - 44. 98% with a little difference, meaning the difference in extreme variation degree is obvious among provenances, and that is not obvious among phenotypic traits. In four leaf traits, there is highly significant negative correlation only between leaf width and ratio of leaf length to leaf width, while there is highly significant positive correlation in other traits. There is a negative correlation between leaf traits and growth traits, while there is a highly significant positive correlation between height and ground diameter. The contribution rate of the first and the second principal components for phenotypic trait variation of Q. acutissima provenances is 43. 663% and 27. 695% , respectively, in which, leaf trait variation plays a dominant role in the first principal component and growth trait variation responses mainly in the second principal component. According to cluster analysis result, twenty-eight geographical provenances can be divided into two groups containing twenty-two and six provenances, respectively, but there is no obvious relationship between cluster analysis result and geographical distribution of provenance. It is suggested that there are rich genetic variations in Q.acutissima provenances, and their phenotypic trait variation exists mostly within provenances.