植物资源与环境学报
植物資源與環境學報
식물자원여배경학보
JOURNAL OF PLANT RESOURCES AND ENVIRONMENT
2014年
3期
24-35
,共12页
赵芹%谢大森%彭庆务%罗少波%郭巨先%邓沙沙
趙芹%謝大森%彭慶務%囉少波%郭巨先%鄧沙沙
조근%사대삼%팽경무%라소파%곽거선%산사사
瓠瓜%ITS 序列%变异位点%同源性%遗传分化%系统发育
瓠瓜%ITS 序列%變異位點%同源性%遺傳分化%繫統髮育
호과%ITS 서렬%변이위점%동원성%유전분화%계통발육
Lagenaria siceraria (Molina) Standl.%ITS sequence%variation site%homology%genetic differentiation%phylogeny
对国产29个瓠瓜也Lagenaria siceraria (Molina) Standl.页品种的 ITS 序列进行了扩增及测序,并结合引自GenBank 的国产9个瓠瓜品种以及国外6个瓠瓜品种和3个同属种类的 ITS 序列,对它们的 ITS 序列长度和 GC 含量以及变异位点进行比较,在此基础上构建系统发育树并对47个样本间的遗传关系进行研究。结果显示：供试47个样本的 ITS 序列均由 ITS1、5.8S rDNA 及 ITS2组成,各样本间的 ITS 序列长度、GC 含量以及变异位点差异明显。国产38个瓠瓜品种的 ITS 序列(包括 ITS1、5.8S rDNA 及 ITS2)长度为619~627 bp、GC 含量为58.00%~63.32%；国外9个样本的 ITS 序列长度为591~626 bp,GC 含量为54.17%~63.26%。序列比对结果显示：国产38个瓠瓜品种的 ITS 序列同源率为84.6%~100.0%,包含221个变异位点；其中,来源于山东的品种‘砧木2’(‘Zhenmu No.2’)的 ITS 序列包含的变异位点最多,与其他品种间的同源率也最低。在系统发育树上,国产38个瓠瓜品种可分为3个分支,来源于山东的品种‘砧木2’和来源于河南的品种‘西瓜砧木1’(‘Xiguazhenmu No.1’)各自聚为第1和第2分支；其余36个品种聚为第3分支。而供试的47个样本则可分为2个分支和5个亚组,第1分支可分为2个亚组,包括国产品种‘砧木2’和产自日本的2个品种；第2分支包含的44个样本则进一步分为3个亚组,国产品种‘西瓜砧木1’和产自法国的品种‘白花瓠瓜’(‘White-flowered gourd’)各自聚为第1和第2亚组,其余的42个样本聚为第3亚组。研究结果表明：供试的不同产地瓠瓜品种间存在丰富的遗传变异和地理分化现象,其 ITS 序列差异与地理分布有一定关系。
對國產29箇瓠瓜也Lagenaria siceraria (Molina) Standl.頁品種的 ITS 序列進行瞭擴增及測序,併結閤引自GenBank 的國產9箇瓠瓜品種以及國外6箇瓠瓜品種和3箇同屬種類的 ITS 序列,對它們的 ITS 序列長度和 GC 含量以及變異位點進行比較,在此基礎上構建繫統髮育樹併對47箇樣本間的遺傳關繫進行研究。結果顯示：供試47箇樣本的 ITS 序列均由 ITS1、5.8S rDNA 及 ITS2組成,各樣本間的 ITS 序列長度、GC 含量以及變異位點差異明顯。國產38箇瓠瓜品種的 ITS 序列(包括 ITS1、5.8S rDNA 及 ITS2)長度為619~627 bp、GC 含量為58.00%~63.32%；國外9箇樣本的 ITS 序列長度為591~626 bp,GC 含量為54.17%~63.26%。序列比對結果顯示：國產38箇瓠瓜品種的 ITS 序列同源率為84.6%~100.0%,包含221箇變異位點；其中,來源于山東的品種‘砧木2’(‘Zhenmu No.2’)的 ITS 序列包含的變異位點最多,與其他品種間的同源率也最低。在繫統髮育樹上,國產38箇瓠瓜品種可分為3箇分支,來源于山東的品種‘砧木2’和來源于河南的品種‘西瓜砧木1’(‘Xiguazhenmu No.1’)各自聚為第1和第2分支；其餘36箇品種聚為第3分支。而供試的47箇樣本則可分為2箇分支和5箇亞組,第1分支可分為2箇亞組,包括國產品種‘砧木2’和產自日本的2箇品種；第2分支包含的44箇樣本則進一步分為3箇亞組,國產品種‘西瓜砧木1’和產自法國的品種‘白花瓠瓜’(‘White-flowered gourd’)各自聚為第1和第2亞組,其餘的42箇樣本聚為第3亞組。研究結果錶明：供試的不同產地瓠瓜品種間存在豐富的遺傳變異和地理分化現象,其 ITS 序列差異與地理分佈有一定關繫。
대국산29개호과야Lagenaria siceraria (Molina) Standl.혈품충적 ITS 서렬진행료확증급측서,병결합인자GenBank 적국산9개호과품충이급국외6개호과품충화3개동속충류적 ITS 서렬,대타문적 ITS 서렬장도화 GC 함량이급변이위점진행비교,재차기출상구건계통발육수병대47개양본간적유전관계진행연구。결과현시：공시47개양본적 ITS 서렬균유 ITS1、5.8S rDNA 급 ITS2조성,각양본간적 ITS 서렬장도、GC 함량이급변이위점차이명현。국산38개호과품충적 ITS 서렬(포괄 ITS1、5.8S rDNA 급 ITS2)장도위619~627 bp、GC 함량위58.00%~63.32%；국외9개양본적 ITS 서렬장도위591~626 bp,GC 함량위54.17%~63.26%。서렬비대결과현시：국산38개호과품충적 ITS 서렬동원솔위84.6%~100.0%,포함221개변이위점；기중,래원우산동적품충‘침목2’(‘Zhenmu No.2’)적 ITS 서렬포함적변이위점최다,여기타품충간적동원솔야최저。재계통발육수상,국산38개호과품충가분위3개분지,래원우산동적품충‘침목2’화래원우하남적품충‘서과침목1’(‘Xiguazhenmu No.1’)각자취위제1화제2분지；기여36개품충취위제3분지。이공시적47개양본칙가분위2개분지화5개아조,제1분지가분위2개아조,포괄국산품충‘침목2’화산자일본적2개품충；제2분지포함적44개양본칙진일보분위3개아조,국산품충‘서과침목1’화산자법국적품충‘백화호과’(‘White-flowered gourd’)각자취위제1화제2아조,기여적42개양본취위제3아조。연구결과표명：공시적불동산지호과품충간존재봉부적유전변이화지리분화현상,기 ITS 서렬차이여지리분포유일정관계。
ITS sequence of 29 cultivars of Lagenaria siceraria (Molina) Standl. in China was amplified and sequenced, and combined with that of 9 cultivars of L. siceraria in China, 6 cultivars of L. siceraria and 3 congeneric species abroad from GenBank, length, GC content and variation site of their ITS sequence were compared. On this basis, phylogenetic tree was constructed and genetic relationship among 47 samples was researched. The results show that ITS sequence of 47 samples all includes ITS1, 5. 8S rDNA and ITS2, and there are obvious differences in length, GC content and variation site of ITS sequence among different samples. Length of ITS sequence (including ITS1, 5. 8S rDNA and ITS2) of 38 cultivars of L. siceraria in China is 619-627 bp, and their GC content is 58. 00% -63. 32% . While ITS sequence length and GC content of 9 samples abroad are 591 -626 bp and 54. 17% -63. 26% , respectively. The result of sequence alignment shows that homological rate of ITS sequence of 38 cultivars of L. siceraria in China is 84. 6% -100. 0% , which includes 221 variation sites. In which there are the most variation sites of ITS sequence and the smallest homological rate in cultivar ‘Zhenmu No. 2’ from Shandong as compared with other cultivars. According to the phylogenetic tree, 38 cultivars of L.siceraria in China can be divided into 3 branches, cultivar ‘Zhenmu No. 2’ from Shandong and cultivar‘Xiguazhenmu No. 1’ from He’ nan are clustered in the first and second branches, respectively, and other 36 cultivars are clustered in the third branch. While 47 samples tested are divided into 2 branches and 5 subgroups. The first branch can be divided into two subgroups, which includes cultivar ‘Zhenmu No. 2’ in China and two cultivars in Japan; and the second branch includes 44 samples which can be divided into 3 subgroups, in which, cultivar ‘ Xiguazhenmu No. 1’ in China and cultivar ‘ White-flowered gourd’ in France are clustered in the first and second subgroups, respectively, and other 42 cultivars are clustered in the third subgroup. It is suggested that there are rich genetic variations and geographic divergences among cultivars of L. siceraria from different origins, and difference of their ITS sequences is related to geographical distribution.
