植物资源与环境学报
植物資源與環境學報
식물자원여배경학보
JOURNAL OF PLANT RESOURCES AND ENVIRONMENT
2014年
3期
15-23
,共9页
李诗川%李妮亚%刘强%陈坚%向敏%王永丹
李詩川%李妮亞%劉彊%陳堅%嚮敏%王永丹
리시천%리니아%류강%진견%향민%왕영단
海桑属%离子积累%光合参数%叶绿素荧光参数%抗氧化酶活性%相关性分析
海桑屬%離子積纍%光閤參數%葉綠素熒光參數%抗氧化酶活性%相關性分析
해상속%리자적루%광합삼수%협록소형광삼수%항양화매활성%상관성분석
Sonneratia Linn. f.%ion accumulation%photosynthetic parameter%chlorophyll fluorescence parameter%antioxidant enzyme activity%correlation analysis
以海桑属(Sonneratia Linn. f.)红树植物无瓣海桑(S. apetala Buch.-Ham.)、海桑也S. caseolaris (Linn.) Engl.页、杯萼海桑(S. alba Smith)、卵叶海桑(S. ovata Backer)、拟海桑(S.× gulngai N. C. Duke et B. R. Jackes)和海南海桑(S.× hainanensis W. C. Ko et al.)为研究对象,比较了根际土壤和叶片中离子含量以及叶片光合和叶绿素荧光参数、叶绿素含量、抗氧化酶活性和 O-·2产生速率的差异,并分析了叶片中 Na+和 Cl-含量与部分生理生化指标的相关性。比较结果表明：海桑和拟海桑叶片中 K+含量最高、Na+和 Cl-含量均显著低于其他种类,但它们的根际土壤中Na+和 Cl-含量却较高。供试6种植物中仅海桑叶片对 Cl-的富集系数小于1,各供试种类对 K+、Na+、Cl-、Ca2+和Mg2+的富集系数均大于1；无瓣海桑对离子的富集系数由大至小依次为 Mg2+、K+、Ca2+、Na+、Cl-,其他种类对离子的富集系数由大至小均依次为 K+、Ca2+、Mg2+、Na+、Cl-。无瓣海桑和海桑的 chla、chlb 和总叶绿素含量差异不显著,但均高于其他种类；供试种类的 chla/ chlb 比值均约为3,可能与海桑属植物为阳生植物有关。无瓣海桑的 Pn、Tr 和Gs 均最高,而杯萼海桑的 Pn、Tr 和 Gs 均最低,但6种植物的 Ci 无明显差异。供试种类的 Fv / Fm、qP、ETR 和ΦPSⅡ均无显著差异,仅部分种类间的 NPQ 差异显著。无瓣海桑叶片中 SOD、CAT 和 POD 活性均显著高于其他种类,但O-·2产生速率最低；而卵叶海桑叶片中 O-·2产生速率最高,其 APX 活性也均显著高于其他种类。相关性分析结果表明：供试6种植物叶片中 Na+和 Cl-含量与 Pn、qP、ΦPSⅡ和 ETR 负相关,与 NPQ 及 SOD、CAT、APX 和 POD 活性正相关。其中,Na+含量与 qP、ΦPSⅡ、ETR 和 SOD 活性极显著相关,与 NPQ 和 CAT 活性显著相关；Cl-含量与 SOD 活性极显著相关,与 qP、ΦPSⅡ和 ETR 显著相关。研究结果表明：供试海桑属植物对高盐生境有不同的耐性机制,其中,海桑和拟海桑通过拒吸 Na+和 Cl-抵御盐胁迫的伤害；供试6种植物对海岸潮间带生境的适应性有明显差异,无瓣海桑最适宜在此生境中生长。
以海桑屬(Sonneratia Linn. f.)紅樹植物無瓣海桑(S. apetala Buch.-Ham.)、海桑也S. caseolaris (Linn.) Engl.頁、杯萼海桑(S. alba Smith)、卵葉海桑(S. ovata Backer)、擬海桑(S.× gulngai N. C. Duke et B. R. Jackes)和海南海桑(S.× hainanensis W. C. Ko et al.)為研究對象,比較瞭根際土壤和葉片中離子含量以及葉片光閤和葉綠素熒光參數、葉綠素含量、抗氧化酶活性和 O-·2產生速率的差異,併分析瞭葉片中 Na+和 Cl-含量與部分生理生化指標的相關性。比較結果錶明：海桑和擬海桑葉片中 K+含量最高、Na+和 Cl-含量均顯著低于其他種類,但它們的根際土壤中Na+和 Cl-含量卻較高。供試6種植物中僅海桑葉片對 Cl-的富集繫數小于1,各供試種類對 K+、Na+、Cl-、Ca2+和Mg2+的富集繫數均大于1；無瓣海桑對離子的富集繫數由大至小依次為 Mg2+、K+、Ca2+、Na+、Cl-,其他種類對離子的富集繫數由大至小均依次為 K+、Ca2+、Mg2+、Na+、Cl-。無瓣海桑和海桑的 chla、chlb 和總葉綠素含量差異不顯著,但均高于其他種類；供試種類的 chla/ chlb 比值均約為3,可能與海桑屬植物為暘生植物有關。無瓣海桑的 Pn、Tr 和Gs 均最高,而杯萼海桑的 Pn、Tr 和 Gs 均最低,但6種植物的 Ci 無明顯差異。供試種類的 Fv / Fm、qP、ETR 和ΦPSⅡ均無顯著差異,僅部分種類間的 NPQ 差異顯著。無瓣海桑葉片中 SOD、CAT 和 POD 活性均顯著高于其他種類,但O-·2產生速率最低；而卵葉海桑葉片中 O-·2產生速率最高,其 APX 活性也均顯著高于其他種類。相關性分析結果錶明：供試6種植物葉片中 Na+和 Cl-含量與 Pn、qP、ΦPSⅡ和 ETR 負相關,與 NPQ 及 SOD、CAT、APX 和 POD 活性正相關。其中,Na+含量與 qP、ΦPSⅡ、ETR 和 SOD 活性極顯著相關,與 NPQ 和 CAT 活性顯著相關；Cl-含量與 SOD 活性極顯著相關,與 qP、ΦPSⅡ和 ETR 顯著相關。研究結果錶明：供試海桑屬植物對高鹽生境有不同的耐性機製,其中,海桑和擬海桑通過拒吸 Na+和 Cl-牴禦鹽脅迫的傷害；供試6種植物對海岸潮間帶生境的適應性有明顯差異,無瓣海桑最適宜在此生境中生長。
이해상속(Sonneratia Linn. f.)홍수식물무판해상(S. apetala Buch.-Ham.)、해상야S. caseolaris (Linn.) Engl.혈、배악해상(S. alba Smith)、란협해상(S. ovata Backer)、의해상(S.× gulngai N. C. Duke et B. R. Jackes)화해남해상(S.× hainanensis W. C. Ko et al.)위연구대상,비교료근제토양화협편중리자함량이급협편광합화협록소형광삼수、협록소함량、항양화매활성화 O-·2산생속솔적차이,병분석료협편중 Na+화 Cl-함량여부분생리생화지표적상관성。비교결과표명：해상화의해상협편중 K+함량최고、Na+화 Cl-함량균현저저우기타충류,단타문적근제토양중Na+화 Cl-함량각교고。공시6충식물중부해상협편대 Cl-적부집계수소우1,각공시충류대 K+、Na+、Cl-、Ca2+화Mg2+적부집계수균대우1；무판해상대리자적부집계수유대지소의차위 Mg2+、K+、Ca2+、Na+、Cl-,기타충류대리자적부집계수유대지소균의차위 K+、Ca2+、Mg2+、Na+、Cl-。무판해상화해상적 chla、chlb 화총협록소함량차이불현저,단균고우기타충류；공시충류적 chla/ chlb 비치균약위3,가능여해상속식물위양생식물유관。무판해상적 Pn、Tr 화Gs 균최고,이배악해상적 Pn、Tr 화 Gs 균최저,단6충식물적 Ci 무명현차이。공시충류적 Fv / Fm、qP、ETR 화ΦPSⅡ균무현저차이,부부분충류간적 NPQ 차이현저。무판해상협편중 SOD、CAT 화 POD 활성균현저고우기타충류,단O-·2산생속솔최저；이란협해상협편중 O-·2산생속솔최고,기 APX 활성야균현저고우기타충류。상관성분석결과표명：공시6충식물협편중 Na+화 Cl-함량여 Pn、qP、ΦPSⅡ화 ETR 부상관,여 NPQ 급 SOD、CAT、APX 화 POD 활성정상관。기중,Na+함량여 qP、ΦPSⅡ、ETR 화 SOD 활성겁현저상관,여 NPQ 화 CAT 활성현저상관；Cl-함량여 SOD 활성겁현저상관,여 qP、ΦPSⅡ화 ETR 현저상관。연구결과표명：공시해상속식물대고염생경유불동적내성궤제,기중,해상화의해상통과거흡 Na+화 Cl-저어염협박적상해；공시6충식물대해안조간대생경적괄응성유명현차이,무판해상최괄의재차생경중생장。
Taking mangrove plants of Sonneratia apetala Buch. -Ham., S. caseolaris (Linn.) Engl., S.alba Smith, S. ovata Backer, S. í gulngai N. C. Duke et B. R. Jackes and S. í hainanensis W. C. Ko et al. in Sonneratia Linn. f. as investigation objects, differences in ion content in rhizosphere soil and leaf and differences in photosynthetic and chlorophyll fluorescence parameters, chlorophyll content, antioxidant enzyme activity and O · 2 production rate in leaf were compared, and correlations of Na+ and Cl--contents in leaf with some physiological and biochemical indexes were analyzed. The comparison results show that K+ content in leaf of S. caseolaris and S. í gulngai is the highest and Na+ and Cl- contents in their leaf are significantly lower than those of other species, but Na+ and Cl- contents in their rhizosphere soil are higher. In these six species, enrichment coefficient of leaf to Cl- is lower than 1 only in S. caseolaris, while that of leaf to K+, Na+, Cl-, Ca2+ and Mg2+ in all species are higher than 1. Order of enrichment coefficient of S. apetala to ions from big to small is Mg2+, K+, Ca2+, Na+, Cl-, while that of other species is K+, Ca2+, Mg2+, Na+, Cl-. Differences in chla, chlb and total chlorophyll contents between S. apetala and S. caseolaris are not significant but are higher than those of other species. Ratios of chla / chlb of all species all are about 3, it may be related to Sonneratia species belonging to heliophyte. Pn, Tr and Gs of S. apetala all are the highest, while those of S. alba all are the lowest, but there is no obvious difference in Ci among all species. There is no significant difference in Fv / Fm, qP, ETR and ΦPSII among all species, there is significant difference only in NPQ among some species. SOD, CAT and POD activities in leaf of S. apetala all are significantly higher than those of other species, but its O 2 production rate is the lowest; while O ·-2 production rate of S. ovata is the highest, and its APX ·-activity is also significantly higher than that of other species. The correlation analysis results show that Na+ and Cl- contents in leaf of six species are negative correlated to Pn, qP, ΦPSII and ETR, and positive correlated to NPQ and SOD, CAT, APX and POD activities. In which, Na+ content is extremely significantly correlated to qP, ΦPSII, ETR and SOD activity, and significantly correlated to NPQ and CAT activity; Cl- content is extremely significantly correlated to SOD activity, and significantly correlated toqP, ΦPSII and ETR. The research results show that there are different tolerant mechanisms of tested Sonneratia species to high salt habitat, in which, S. caseplaris and S. í gulngai can resist damage of salt stress through refusing to absorb Na+ and Cl-. There are obvious differences in adaptability of six tested species to coast intertidal zone habitat, and S. apetala is the most suitable to grow in this habitat.
