半枝莲和白花蛇舌草总多糖对肝癌脾虚证候动物模型的干预研究
반지련화백화사설초총다당대간암비허증후동물모형적간예연구
Anti-tumor effects of polysaccharides from scutellaria barbata D.Don and hedyotis diffusa willd on liver cancer PIXU syndrome in animal model
  • 万方数据
    中国医药 중국의약
    CHINA MEDICINE
    2014年 10期 1501-1505 ,共5页

    陈玉%冯大刚%罗先钦%胡荣

    진옥%풍대강%라선흠%호영

    半枝莲%白花蛇舌草%证候动物模型 半枝蓮%白花蛇舌草%證候動物模型
    반지련%백화사설초%증후동물모형
    Scutellaria barbata%Spreading hedyotis herb%Symptomatic animal model
    目的 探讨半枝莲和白花蛇舌草总多糖对肝癌(H22)脾虚证候动物模型的影响.方法 选无特定病原体级昆明小鼠36只(体质量18～22 g),完全随机分为正常对照组(6只)、脾虚模型组(6只)、环磷酰胺组(6只)和总多糖组(18只:高、中、低浓度各6只).制备质量比为1∶1的半枝莲和白花蛇舌草总多糖,除正常对照组外其余3组均制备为脾虚证候动物模型(大黄+芒硝),4组均接种肝癌H22细胞.正常对照组和脾虚模型组给予蒸馏水0.4 ml/(次·只)灌胃,环磷酰胺组和高、中、低浓度总多糖组分别给予环磷酰胺25 mg/kg和总多糖200、100、50 mg/kg,观察荷瘤小鼠体质量、肛温、胸腺与脾脏指数的变化,检测白细胞介素2(IL-2)、肿瘤坏死因子α(TNF-α)、T淋巴细胞亚群(CD3+、CD4+、CD8+百分比,CD4 +/CD8+比值)水平.结果 造模后及药物干预后,脾虚模型组小鼠体质量和肛温均低于正常对照组,组间差异均有统计学意义[造模后,体质量:(26.2±1.0)g比(32.7±1.0)g,肛温:(36.53±0.27)℃比(37.39±0.35)℃;药物干预后,体质量:(29.5±2.9)g比(36.0 ±2.8)g,肛温:(36.87±0.29)℃比(37.43±0.39)℃;均P＜0.05].药物干预后,环磷酰胺组和总多糖组(高、中浓度)小鼠体质量均高于脾虚模型组,差异有统计学意义[(32.6±2.8)、(33.6±2.6)、(32.2 ±2.6)g比(29.5±2.9)g,P＜0.05].环磷酰胺组和总多糖组(高浓度)小鼠肛温高于脾虚模型组,差异有统计学意义[(37.23 ±0.32)、(37.18 ±0.28)℃比(36.87±0.29)℃,P＜0.05].与脾虚模型组比较,环磷酰胺组和总多糖组(高、中、低浓度)小鼠瘤体重量明显降低,差异有统计学意义[(2.0±0.6)、(4.6±l.4)、(5.0±1.9)、(6.0±2.2)g比(9.8 ±2.4)g,P＜0.01];总多糖组(高、中浓度)、正常对照组小鼠胸腺指数高于脾虚模型组,差异有统计学意义[(2.59±0.41)、(2.41 ±0.57)、(2.85±0.72)比(1.87±0.61),P＜0.01或P＜0.05];总多糖组(高浓度)、正常对照组小鼠脾脏指数高于脾虚模型组,差异有统计学意义[(7.5±2.0)、(8.7±2.3)比(5.9±1.4),P＜0.01或P＜0.05].与脾虚模型组比较,总多糖组(高、中浓度)小鼠外周血IL-2和TNF-α含量明显升高[IL-2:(1.79±0.32)、(1.52±0.26)μg/L比(1.07±0.26) μg/L,TNF-α:(0.96±0.18)、(0.86 ±0.20) μg/L比(0.63 ±0.21) μg/L,P＜0.01或P＜0.05].总多糖组(高浓度)小鼠外周血中T淋巴细胞CD3+、CD4+总数高于脾虚模型组[CD3+:(62±16)％比(46±11)％,CD4+:(30±4)％比(24±6)％,均P＜0.05],总多糖组(高、中浓度)小鼠外周血中T淋巴细胞CD8+总数低于脾虚模型组[(24±5)％、(24±6)％比(31±8)％,P＜0.05],总多糖组(高、中、低浓度)小鼠外周血中T淋巴细胞CD4 +/CD8+比例均高于脾虚模型组[(1.26±0.35)、(1.18±0.29)、(1.02±0.41)比(0.77±0.49),P＜0.01或P＜0.05].结论 半枝莲和白花蛇舌草总多糖对肝癌(H22)脾虚证候小鼠模型具有较强抗肿瘤作用,并可以提高其免疫系统功能.
    목적 탐토반지련화백화사설초총다당대간암(H22)비허증후동물모형적영향.방법 선무특정병원체급곤명소서36지(체질량18～22 g),완전수궤분위정상대조조(6지)、비허모형조(6지)、배린선알조(6지)화총다당조(18지:고、중、저농도각6지).제비질량비위1∶1적반지련화백화사설초총다당,제정상대조조외기여3조균제비위비허증후동물모형(대황+망초),4조균접충간암H22세포.정상대조조화비허모형조급여증류수0.4 ml/(차·지)관위,배린선알조화고、중、저농도총다당조분별급여배린선알25 mg/kg화총다당200、100、50 mg/kg,관찰하류소서체질량、항온、흉선여비장지수적변화,검측백세포개소2(IL-2)、종류배사인자α(TNF-α)、T림파세포아군(CD3+、CD4+、CD8+백분비,CD4 +/CD8+비치)수평.결과 조모후급약물간예후,비허모형조소서체질량화항온균저우정상대조조,조간차이균유통계학의의[조모후,체질량:(26.2±1.0)g비(32.7±1.0)g,항온:(36.53±0.27)℃비(37.39±0.35)℃;약물간예후,체질량:(29.5±2.9)g비(36.0 ±2.8)g,항온:(36.87±0.29)℃비(37.43±0.39)℃;균P＜0.05].약물간예후,배린선알조화총다당조(고、중농도)소서체질량균고우비허모형조,차이유통계학의의[(32.6±2.8)、(33.6±2.6)、(32.2 ±2.6)g비(29.5±2.9)g,P＜0.05].배린선알조화총다당조(고농도)소서항온고우비허모형조,차이유통계학의의[(37.23 ±0.32)、(37.18 ±0.28)℃비(36.87±0.29)℃,P＜0.05].여비허모형조비교,배린선알조화총다당조(고、중、저농도)소서류체중량명현강저,차이유통계학의의[(2.0±0.6)、(4.6±l.4)、(5.0±1.9)、(6.0±2.2)g비(9.8 ±2.4)g,P＜0.01];총다당조(고、중농도)、정상대조조소서흉선지수고우비허모형조,차이유통계학의의[(2.59±0.41)、(2.41 ±0.57)、(2.85±0.72)비(1.87±0.61),P＜0.01혹P＜0.05];총다당조(고농도)、정상대조조소서비장지수고우비허모형조,차이유통계학의의[(7.5±2.0)、(8.7±2.3)비(5.9±1.4),P＜0.01혹P＜0.05].여비허모형조비교,총다당조(고、중농도)소서외주혈IL-2화TNF-α함량명현승고[IL-2:(1.79±0.32)、(1.52±0.26)μg/L비(1.07±0.26) μg/L,TNF-α:(0.96±0.18)、(0.86 ±0.20) μg/L비(0.63 ±0.21) μg/L,P＜0.01혹P＜0.05].총다당조(고농도)소서외주혈중T림파세포CD3+、CD4+총수고우비허모형조[CD3+:(62±16)％비(46±11)％,CD4+:(30±4)％비(24±6)％,균P＜0.05],총다당조(고、중농도)소서외주혈중T림파세포CD8+총수저우비허모형조[(24±5)％、(24±6)％비(31±8)％,P＜0.05],총다당조(고、중、저농도)소서외주혈중T림파세포CD4 +/CD8+비례균고우비허모형조[(1.26±0.35)、(1.18±0.29)、(1.02±0.41)비(0.77±0.49),P＜0.01혹P＜0.05].결론 반지련화백화사설초총다당대간암(H22)비허증후소서모형구유교강항종류작용,병가이제고기면역계통공능.
    Objective To investigate the effect of scutellaria barbata and hedyotis diffusa on symptom of PI XU in animal model.Methods Thirty-six mice model with hepatic cancer which got a symptom of PI XU were established by gavage and mixture of Da Huang and Mang Xiao; mice models were randomized divided into 6 groups.Normal group,PI XU group and experimental group were given different doses (200,100,50 mg/kg) of Scutellaria barbata,Hedyotis diffusa and cyclophosphamide (CTX).Control group was given physiological saline and CTX.We observed the growth of tumor,inhibition rate of tumor,polysaccharides of thymus and spleen index.Interleukin-2(IL-2),tumor necrosis factor(TNF-α),sub-group of T lymphocyte (percentage of CD3 +,CD4 +,CD8 + and rate of CD4 +/CD8 +) were detected.Results After modeling and drug intervention,in PI XU group body weight and anal temperature were lower than those in the normal group; the differences were statistically significant [body weight:(26.2 ± 1.0) g vs (32.7 ± 1.0) g,(29.5 ± 2.9) g vs (36.0 ± 2.8) g; anal temperature:(36.53 ± 0.27) ℃ vs (37.39 ± 0.35) ℃,(36.87 ± 0.29) ℃ vs (37.43 ± 0.39) ℃] (all P ＜ 0.05).After drug intervention,in CTX group and total polysaccharide group (high,medium density) the body weight were higher than those in PI XU group ; the difference were statistically significant [(32.6 ± 2.8) g,(33.6 ± 2.6) g,(32.2 ± 2.6)g vs (29.5 ±2.9)g,all P ＜0.05].In CTX group and total polysaccharide group(high density) the anal temperature higher than that in PI XU group ; the difference were statistically significant [(37.23 ± 0.32) ℃,(37.18 ± 0.28) ℃ vs (36.87 ± 0.29) ℃,P ＜ 0.05].In CTX and total polysaccharide groups (high,medium and low density) the tumor weight were significantly lower than PI XU group; the difference were statistically significant[(2.0±0.6)g,(4.6±1.4)g,(5.0±1.9)g,(6.0±2.2)gvs (9.8±2.4)g,P＜0.01].The tumorburdened mice thymus index [(2.59 ±0.41),(2.41 ± 0.57),(2.85 ± 0.72) vs (1.87 ± 0.61),P ＜ 0.01 or P ＜ 0.05] and spleen index [(7.5 ± 2.0),(8.7 ± 2.3) vs (5.9 ± 1.4),P ＜ 0.01 or P ＜ 0.05] increased significantly in tumor-burdened mice.Peripheral blood IL-2 and TNF-α increased significantly [IL-2:(1.79 ±0.32) μg/L,(1.52 ± 0.26) μg/L vs (1.07 ± 0.26) μg/L,TNF-α:(0.96 ± 0.18) μg/L,(0.86 ± 0.20) μg/L vs (0.63 ±0.21) μg,/L,P ＜0.01 or P ＜0.05] in total polysaccharide group of spleen deficiency syndrome of liver cancer mice.CD3 +,CD4 + T lymphocytes [CD3 +:(62 ± 16) ％ vs (46 ± 11) ％,CD4 +:(30 ± 4) ％ vs (24 ±6)％,all P ＜0.05] and CD4 +/CD8 + [(1.26 ±0.35),(1.18 ±0.29),(1.02 ±0.41)vs (0.77 ±0.49),P ＜0.01 or P ＜ 0.05] in peripheral blood increased significantly in total polysaccharide group of spleen deficiency syndrome.Conclusion Scutellaria barbata and Hedyotis diffusa can improve the immune function in hepatic cancer mice mode with symptom of PI XU.
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