CAJ | 학술논문

目的：通过单次腹腔注射不同剂量的四氯化碳（CCl4）致小鼠急性肝损伤，检测小鼠血浆转氨酶水平的变化，建立适合本研究的小鼠模型。方法采用6～8周龄雄性C57BL/6小鼠单次腹腔注射CCl4诱导急性肝损伤。比较不同剂量的CCl4诱导急性肝损伤小鼠的血浆转氨酶水平。将182只小鼠随机分出32只。32只小鼠随机分为3组CCl4实验组（每组8只）和正常对照组（8只），分别腹腔注射0.1%、0.2%、0.3%的CCl4橄榄油溶液（10 ml/kg）或等体积橄榄油。注射12小时后取血，检测血浆丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）的水平。比较0.1%和0.2%剂量组小鼠不同时间点血浆转氨酶水平的变化。将剩余150只小鼠随机分为正常对照组（10只）、实验Ⅰ组（0.1%剂量，70只）、实验Ⅱ组（0.2%剂量，70只），实验组小鼠在造模6、12、16、20、24、48、72小时后取血，检测血浆ALT、AST的水平。结果与正常对照组相比，分别用0.1%、0.2%、0.3% CCl4处理后12小时小鼠血浆ALT、AST均明显升高（P ＜0.05），并呈剂量依赖性。与正常对照组相比，0.1%和0.2%剂量组小鼠血浆ALT、AST的水平在早期均呈上升趋势，并分别在16小时和20小时达到高峰，随后逐渐下降，在72小时接近至正常水平。结论单次腹腔注射CCl4诱导小鼠急性肝损伤呈剂量依赖性。0.1%剂量的CCl4以及该剂量处理后16小时是建立C57BL/6小鼠急性轻度肝损伤的合适剂量与检测转氨酶水平的合适时间点。
목적：통과단차복강주사불동제량적사록화탄（CCl4）치소서급성간손상，검측소서혈장전안매수평적변화，건립괄합본연구적소서모형。방법채용6～8주령웅성C57BL/6소서단차복강주사CCl4유도급성간손상。비교불동제량적CCl4유도급성간손상소서적혈장전안매수평。장182지소서수궤분출32지。32지소서수궤분위3조CCl4실험조（매조8지）화정상대조조（8지），분별복강주사0.1%、0.2%、0.3%적CCl4감람유용액（10 ml/kg）혹등체적감람유。주사12소시후취혈，검측혈장병안산전안매（ALT）、천동안산전안매（AST）적수평。비교0.1%화0.2%제량조소서불동시간점혈장전안매수평적변화。장잉여150지소서수궤분위정상대조조（10지）、실험Ⅰ조（0.1%제량，70지）、실험Ⅱ조（0.2%제량，70지），실험조소서재조모6、12、16、20、24、48、72소시후취혈，검측혈장ALT、AST적수평。결과여정상대조조상비，분별용0.1%、0.2%、0.3% CCl4처리후12소시소서혈장ALT、AST균명현승고（P ＜0.05），병정제량의뢰성。여정상대조조상비，0.1%화0.2%제량조소서혈장ALT、AST적수평재조기균정상승추세，병분별재16소시화20소시체도고봉，수후축점하강，재72소시접근지정상수평。결론단차복강주사CCl4유도소서급성간손상정제량의뢰성。0.1%제량적CCl4이급해제량처리후16소시시건립C57BL/6소서급성경도간손상적합괄제량여검측전안매수평적합괄시간점。
Objective To establish an appropriate murine model for this study, the mice were administrated by single intraperitoneal injection at different doses with carbon tetrachloride (CCl4) to induce acute liver injury, and the changes of aminotransferase levels in plasma were measured. Methods Acute liver injury in C57BL/6 male mice (6-8 weeks) were administrated by single intraperitoneal injection with CCl4. Comparing the aminotransferase levels in plasma from different doses of CCl4-injected mice. The 32 mice were randomly separated from 182 mice. The 32 mice were randomly divided into normal control group (8 mice) and 0.1%, 0.2% and 0.3% CCl4 groups (8 mice per group) which received 0.1%, 0.2% and 0.3% CCl4 (10 ml/kg, dissolved in olive oil) respectively, while the normal control group received only olive oil as control. Twelve hours after challenge, the mice were sacrificed and plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. Comparing the aminotransferase levels in plasma from 0.1%and 0.2%groups at different time points. The 150 mice were randomly divided into normal control group (8 mice), the experimental groupⅠ(0.1%dose, 70 mice) and the experimental groupⅡ(0.2%dose, 70 mice). Plasma levels of ALT and AST were measured at 6, 12, 16, 20, 24, 48 and 72 hours after challenge. Results Compared with normal control group, plasma ALT and AST were signiifcantly increased (P<0.05) in CCl4-treated mice at 12 hour, furthermore, the effect was dose-dependent in 0.1%, 0.2%and 0.3%groups. The levels of ALT and AST rised at the early phase in 0.1%and 0.2%groups, and reached the peak at 16 and 20 hours, respectively. Then the levels declined gradually and were close to the normal level at 72 hours. Conclusions The increase of ALT and AST levels was in a dose-dependent manner with CCl4 by single intraperitoneal injection. To establish acute mild liver injury in C57BL/6 mice, 0.1%of CCl4 was the appropriate dose, and 16 hours after challenge was the suitable time point for detection of aminotransferase levels.