CAJ | 학술논문

为了研究类球红细菌SOD的提取工艺，采用研磨法、超声波法、冻融辅助超声波法和酶法辅助超声波法提取类球红细菌超氧化物歧化酶，结果表明：酶法辅助超声波法是较优的提取方法。对类球红细菌SOD的提取工艺进行单因素和正交试验，得到较优的提取工艺参数为：超声波输出功率500W，超声波工作总时间15min，固液比1：15，酶添加量300μL，酶解温度40℃，酶解pH值7，酶解时间60min。在此较优提取工艺参数下，类球红细菌SOD活性达到385.7 U·mL-1，较优化前提高40%。
위료연구류구홍세균SOD적제취공예，채용연마법、초성파법、동융보조초성파법화매법보조초성파법제취류구홍세균초양화물기화매，결과표명：매법보조초성파법시교우적제취방법。대류구홍세균SOD적제취공예진행단인소화정교시험，득도교우적제취공예삼수위：초성파수출공솔500W，초성파공작총시간15min，고액비1：15，매첨가량300μL，매해온도40℃，매해pH치7，매해시간60min。재차교우제취공예삼수하，류구홍세균SOD활성체도385.7 U·mL-1，교우화전제고40%。
Extraction technique of SOD from Rhodobacter sphaeroides was studied by ultrasonic assisted with zymolysis. By comparision with grinding, ultrasonic and ultrasonic assisted with freezing-thawing, ultrasonic assisted with zymolysis was the optimum extraction method for superoxide dismutase (SOD) from Rhodobacter sphaeroides 3757. Results of both single factor experiment and orthogonal test showed that the optimum ultrasonic assisted with zymolysis extraction conditions of SOD were as follows: ultrasonic power 500W, ultrasonic total working time 15min, material/liquid ratio 1:15, amount of enzyme 300 μL, temperature of zymoIysis 40℃, pH of zymoIysis 7, time of zymoIysis 60 min. Under these optimum conditions, activities of SOD was 385.7 U·mL-1, and the extraction rate would be improved by 40%.