组织工程与重建外科杂志
組織工程與重建外科雜誌
조직공정여중건외과잡지
JOURNAL OF TISSUE ENGINEERING AND RECONSTRUCTIVE SURGERY
2014年
2期
73-76
,共4页
郭相凯%韩志华%段惠川%钱德检%郗林鹤%孟飞%刘炬%王彦
郭相凱%韓誌華%段惠川%錢德檢%郗林鶴%孟飛%劉炬%王彥
곽상개%한지화%단혜천%전덕검%치림학%맹비%류거%왕언
胚胎皮肤细胞%再生皮肤%瘢痕形成
胚胎皮膚細胞%再生皮膚%瘢痕形成
배태피부세포%재생피부%반흔형성
Embryonic skin cell%Regenerative skin%Scar formation
目的:探索胚胎皮肤细胞修复小鼠背部皮肤缺损的疗效。方法建立小鼠皮肤损伤模型,将小鼠胚胎皮肤的细胞植入小鼠皮肤损伤处(Nor-T组,n=6),与不作处理的小鼠(Nor组,n=6)进行皮肤损伤愈合的比较。分别在术后第1、2、3周采集标本,比较Nor-T组与Nor组创面愈合的情况,以免疫组织化学方法检测各组愈合过程中,TGF-β1和TGF-β3因子的表达情况。结果术后第1周,Nor-T组在创伤处形成再生皮肤覆盖创面;Nor组创面由痂皮覆盖;术后第2周,Nor-T组再生皮肤完全形成;Nor组瘢痕愈合。术后第3周,Nor-T组再生皮肤上生长出黑色毛发;而Nor组没有毛发生长。Nor-T组中,TGF-β1在术后第1周表达阳性,TGF-β3在术后第2周表达阳性。结论胚胎皮肤细胞具有形成再生皮肤和修复皮肤缺损的能力。
目的:探索胚胎皮膚細胞脩複小鼠揹部皮膚缺損的療效。方法建立小鼠皮膚損傷模型,將小鼠胚胎皮膚的細胞植入小鼠皮膚損傷處(Nor-T組,n=6),與不作處理的小鼠(Nor組,n=6)進行皮膚損傷愈閤的比較。分彆在術後第1、2、3週採集標本,比較Nor-T組與Nor組創麵愈閤的情況,以免疫組織化學方法檢測各組愈閤過程中,TGF-β1和TGF-β3因子的錶達情況。結果術後第1週,Nor-T組在創傷處形成再生皮膚覆蓋創麵;Nor組創麵由痂皮覆蓋;術後第2週,Nor-T組再生皮膚完全形成;Nor組瘢痕愈閤。術後第3週,Nor-T組再生皮膚上生長齣黑色毛髮;而Nor組沒有毛髮生長。Nor-T組中,TGF-β1在術後第1週錶達暘性,TGF-β3在術後第2週錶達暘性。結論胚胎皮膚細胞具有形成再生皮膚和脩複皮膚缺損的能力。
목적:탐색배태피부세포수복소서배부피부결손적료효。방법건립소서피부손상모형,장소서배태피부적세포식입소서피부손상처(Nor-T조,n=6),여불작처리적소서(Nor조,n=6)진행피부손상유합적비교。분별재술후제1、2、3주채집표본,비교Nor-T조여Nor조창면유합적정황,이면역조직화학방법검측각조유합과정중,TGF-β1화TGF-β3인자적표체정황。결과술후제1주,Nor-T조재창상처형성재생피부복개창면;Nor조창면유가피복개;술후제2주,Nor-T조재생피부완전형성;Nor조반흔유합。술후제3주,Nor-T조재생피부상생장출흑색모발;이Nor조몰유모발생장。Nor-T조중,TGF-β1재술후제1주표체양성,TGF-β3재술후제2주표체양성。결론배태피부세포구유형성재생피부화수복피부결손적능력。
Objective To explore the efficacy of repairing skin defect of mouse by using embryonic skin cell. Methods Animal model of back skin damage in mice was built. The embryonic skin cells were transplanted into the back skin damage of mice as treatment group (Nor-T group, n=6) and the mice in control group (Nor group, n=6) were received no treatment. The wound healing situation were observed 1, 2 and 3 weeks after operation. The expression of TGF-βwas detected 1 and 2 weeks after operation. Results The regenerative skin growth was observed on the wound area in Nor-T group, while only crusta was observed on the wound area in Nor group 1 week after operation. The regenerative skin merged with surrounding skin in Nor-T group, while the wound healed with scar in in Nor group 2 weeks after operation. The hair was observed in Nor-T group, while no hair was observed in Nor group 3 weeks after operation. In Nor-T group, TGF-β1 was expressed 1 week after operation and TGF- β3 was expressed 2 week after operation. Conclusion The embryonic skin cells possess the ability of regenerative skin formation, and TGF-βhas different expression at different wound healing phase.