中国小儿血液与肿瘤杂志
中國小兒血液與腫瘤雜誌
중국소인혈액여종류잡지
JOURNAL OF CHINA PEDIATRIC BLOOD AND CANCER
2014年
2期
85-89
,共5页
肾母细胞瘤SK-NEP-1细胞%苦参碱%顺铂%程序性细胞死亡
腎母細胞瘤SK-NEP-1細胞%苦參堿%順鉑%程序性細胞死亡
신모세포류SK-NEP-1세포%고삼감%순박%정서성세포사망
wilms tumor%matrine%cisplatin%programmed cell death
目的：探讨苦参碱及苦参碱联合顺铂对人肾母细胞瘤SK-NEP-1细胞存活及凋亡的影响，以及可能的作用机制。方法苦参碱、顺铂单独及联合作用SK-NEP-1细胞，实验分对照组、顺铂干预组、苦参碱干预组、苦参碱联合顺铂干预组。应用四甲基偶氮唑蓝比色法检测SK-NEP-1细胞的存活率，用流式细胞仪检测其凋亡率，逆转录聚合酶链反应法检测其PDCD4 mRNA的表达。结果各组SK-NEP-1细胞的存活率及凋亡率与对照组比较、相同浓度的苦参碱干预组与苦参碱联合顺铂干预组比较及苦参碱联合顺铂组与顺铂干预组比较，SK-NEP-1细胞的存活率及凋亡率差异均有显著性（P＜0.05）。各实验组均能提高PDCD4 mRNA的表达，与对照组比较，差异有显著性（P＜0.05）。苦参碱联合顺铂组与苦参碱干预组及顺铂干预组比较，SK-NEP-1细胞 PDCD4 mRNA表达均显著提高（P＜0.05）。结论苦参碱可浓度依赖性的抑制SK-NEP-1细胞的增殖并诱导其凋亡，从而提高对顺铂化疗的敏感性，该作用可能与提高细胞内PDCD4 mRNA的表达有关。
目的：探討苦參堿及苦參堿聯閤順鉑對人腎母細胞瘤SK-NEP-1細胞存活及凋亡的影響，以及可能的作用機製。方法苦參堿、順鉑單獨及聯閤作用SK-NEP-1細胞，實驗分對照組、順鉑榦預組、苦參堿榦預組、苦參堿聯閤順鉑榦預組。應用四甲基偶氮唑藍比色法檢測SK-NEP-1細胞的存活率，用流式細胞儀檢測其凋亡率，逆轉錄聚閤酶鏈反應法檢測其PDCD4 mRNA的錶達。結果各組SK-NEP-1細胞的存活率及凋亡率與對照組比較、相同濃度的苦參堿榦預組與苦參堿聯閤順鉑榦預組比較及苦參堿聯閤順鉑組與順鉑榦預組比較，SK-NEP-1細胞的存活率及凋亡率差異均有顯著性（P＜0.05）。各實驗組均能提高PDCD4 mRNA的錶達，與對照組比較，差異有顯著性（P＜0.05）。苦參堿聯閤順鉑組與苦參堿榦預組及順鉑榦預組比較，SK-NEP-1細胞 PDCD4 mRNA錶達均顯著提高（P＜0.05）。結論苦參堿可濃度依賴性的抑製SK-NEP-1細胞的增殖併誘導其凋亡，從而提高對順鉑化療的敏感性，該作用可能與提高細胞內PDCD4 mRNA的錶達有關。
목적：탐토고삼감급고삼감연합순박대인신모세포류SK-NEP-1세포존활급조망적영향，이급가능적작용궤제。방법고삼감、순박단독급연합작용SK-NEP-1세포，실험분대조조、순박간예조、고삼감간예조、고삼감연합순박간예조。응용사갑기우담서람비색법검측SK-NEP-1세포적존활솔，용류식세포의검측기조망솔，역전록취합매련반응법검측기PDCD4 mRNA적표체。결과각조SK-NEP-1세포적존활솔급조망솔여대조조비교、상동농도적고삼감간예조여고삼감연합순박간예조비교급고삼감연합순박조여순박간예조비교，SK-NEP-1세포적존활솔급조망솔차이균유현저성（P＜0.05）。각실험조균능제고PDCD4 mRNA적표체，여대조조비교，차이유현저성（P＜0.05）。고삼감연합순박조여고삼감간예조급순박간예조비교，SK-NEP-1세포 PDCD4 mRNA표체균현저제고（P＜0.05）。결론고삼감가농도의뢰성적억제SK-NEP-1세포적증식병유도기조망，종이제고대순박화료적민감성，해작용가능여제고세포내PDCD4 mRNA적표체유관。
Objective Objective To explore the effects of matrine and cisplatin on survival and apoptosis of nephroblastoma cell line SK-NEP-1 , and its potential mechanism. Methods SK-NEP-1 cells were treated with different concentration of Matrine and cisplatin individually or in combination.Trials were divided into following groups:control group,cisplatin intervention group, matrine intervention group,and matrine combined with cisplatin intervention group.The survival rate of SK-NEP-1 cells treated with different concentration of Matrine and cisplatin was detected by MTT colorimetric assay.Apoptosis rate of them by flow cytometry(FCM).RT-PCR analysis was employed to measure the PDCD4 mRNA expression.Results There was significant difference in survival and apoptosis of SK-NEP-1 cells between each group and control group (P<0.05 ),The survival rate and apoptosis of SK-NEP-1 cells was statistically significant between Matrine group and Matrine combined with cisplation group (P <0.05 );and also between the Matrine joint cisplation group and cisplation intervention group(P <0.05 ).Compared with control group ,the expression of PDCD4 mRNA was increased significantly in matrine intervention group,cisplatin intervention group,and matrine combined with cisplatin intervention group (P <0.05 ).Combined with matrine and cisplation individual drug group,the expression of PDCD4 mRNA was increased significantly in matrine combined with cisplatin intervention group.Conclusion Matrine could induce apoptosis and inhibit the growth of SK-NEP-1 cells in a does-dependent way .Matrine could increase the sensibility of chemotherapy of cisplatin and this effect is correlated to increase expression of PDCD4.
