中国小儿血液与肿瘤杂志
中國小兒血液與腫瘤雜誌
중국소인혈액여종류잡지
JOURNAL OF CHINA PEDIATRIC BLOOD AND CANCER
2014年
2期
69-72
,共4页
王舒静%高怡瑾%孟建华%陆凤娟%李军%王宏胜%翟晓文%苗慧%钱晓文%俞懿
王舒靜%高怡瑾%孟建華%陸鳳娟%李軍%王宏勝%翟曉文%苗慧%錢曉文%俞懿
왕서정%고이근%맹건화%륙봉연%리군%왕굉성%적효문%묘혜%전효문%유의
B细胞非霍奇金淋巴瘤%B细胞白血病/淋巴瘤-2%BCL-6基因%儿童
B細胞非霍奇金淋巴瘤%B細胞白血病/淋巴瘤-2%BCL-6基因%兒童
B세포비곽기금림파류%B세포백혈병/림파류-2%BCL-6기인%인동
B-cell Non-Hodgkin′s lymphomas%BCL-2%BCL-6%Pediatric
目的初步探讨儿童成熟B细胞非霍奇金淋巴瘤(B-NHL)中BCL-2、BCL-6蛋白表达及其临床病理意义。方法收集1999-2011年复旦大学附属儿科医院血液科收治的92例初治儿童B-NHL资料,包括伯基特淋巴瘤(burkitl lymphoma,BL)53例,弥漫大B细胞性淋巴瘤(DLBCL)38例,以及介于BL与DLBCL之间未能分类的B细胞淋巴瘤(BL/DLBCL)1例。92例患儿年龄≤16岁。所有病例均经过2家三级甲等医院病理科诊断。通过免疫组织化学技术检测儿童B-NHL中BCL-2、BCL-6蛋白表达情况。结果(1)92例儿童B-NHL中47例进行BCL-2蛋白检测,BCL-2表达阳性率在BL和DLBCL中分别为9.7%(3/31)和33.3%(5/15),差异具有统计学意义(P=0.047)。BCL-2蛋白表达与性别、临床分期及预后无相关性(P>0.05)。(2)31例儿童B-NHL进行BCL-6蛋白检测,BCL-6表达阳性组与阴性组2年EFS 分别为83.3%和45.5%,差异有统计学意义(P=0.019);在临床分期为Ⅲ-Ⅳ期儿童B-NHL中,BCL-6阳性组与阴性组的2年EFS分别为80%和40%,2组差异具有统计学意义(P=0.023)。结论儿童B-NHL中BCL-2蛋白表达与病理类型有关,BL中BCL-2蛋白一般表达阴性,可用于BL 与 DLBCL 之间的鉴别;儿童 B-NHL 中BCL-6表达阳性的患儿预后较好,提示BCL-6可能是B-NHL的预后因素之一。
目的初步探討兒童成熟B細胞非霍奇金淋巴瘤(B-NHL)中BCL-2、BCL-6蛋白錶達及其臨床病理意義。方法收集1999-2011年複旦大學附屬兒科醫院血液科收治的92例初治兒童B-NHL資料,包括伯基特淋巴瘤(burkitl lymphoma,BL)53例,瀰漫大B細胞性淋巴瘤(DLBCL)38例,以及介于BL與DLBCL之間未能分類的B細胞淋巴瘤(BL/DLBCL)1例。92例患兒年齡≤16歲。所有病例均經過2傢三級甲等醫院病理科診斷。通過免疫組織化學技術檢測兒童B-NHL中BCL-2、BCL-6蛋白錶達情況。結果(1)92例兒童B-NHL中47例進行BCL-2蛋白檢測,BCL-2錶達暘性率在BL和DLBCL中分彆為9.7%(3/31)和33.3%(5/15),差異具有統計學意義(P=0.047)。BCL-2蛋白錶達與性彆、臨床分期及預後無相關性(P>0.05)。(2)31例兒童B-NHL進行BCL-6蛋白檢測,BCL-6錶達暘性組與陰性組2年EFS 分彆為83.3%和45.5%,差異有統計學意義(P=0.019);在臨床分期為Ⅲ-Ⅳ期兒童B-NHL中,BCL-6暘性組與陰性組的2年EFS分彆為80%和40%,2組差異具有統計學意義(P=0.023)。結論兒童B-NHL中BCL-2蛋白錶達與病理類型有關,BL中BCL-2蛋白一般錶達陰性,可用于BL 與 DLBCL 之間的鑒彆;兒童 B-NHL 中BCL-6錶達暘性的患兒預後較好,提示BCL-6可能是B-NHL的預後因素之一。
목적초보탐토인동성숙B세포비곽기금림파류(B-NHL)중BCL-2、BCL-6단백표체급기림상병리의의。방법수집1999-2011년복단대학부속인과의원혈액과수치적92례초치인동B-NHL자료,포괄백기특림파류(burkitl lymphoma,BL)53례,미만대B세포성림파류(DLBCL)38례,이급개우BL여DLBCL지간미능분류적B세포림파류(BL/DLBCL)1례。92례환인년령≤16세。소유병례균경과2가삼급갑등의원병이과진단。통과면역조직화학기술검측인동B-NHL중BCL-2、BCL-6단백표체정황。결과(1)92례인동B-NHL중47례진행BCL-2단백검측,BCL-2표체양성솔재BL화DLBCL중분별위9.7%(3/31)화33.3%(5/15),차이구유통계학의의(P=0.047)。BCL-2단백표체여성별、림상분기급예후무상관성(P>0.05)。(2)31례인동B-NHL진행BCL-6단백검측,BCL-6표체양성조여음성조2년EFS 분별위83.3%화45.5%,차이유통계학의의(P=0.019);재림상분기위Ⅲ-Ⅳ기인동B-NHL중,BCL-6양성조여음성조적2년EFS분별위80%화40%,2조차이구유통계학의의(P=0.023)。결론인동B-NHL중BCL-2단백표체여병리류형유관,BL중BCL-2단백일반표체음성,가용우BL 여 DLBCL 지간적감별;인동 B-NHL 중BCL-6표체양성적환인예후교호,제시BCL-6가능시B-NHL적예후인소지일。
Objective The study was designed to investigate the protein expression of BCL-2 and BCL-6 in children with B-NHL and its clinic-pathological significance.Method Between July 1999 and October 2011,92 untreated patients (age 16 years or less)with newly diagnosed B-NHL (including BL, DLBCL and BL/DLBCL)were enrolled.We use the immunohistochemical technique (Envision TM)to detect BCL-2,BCL-6 protein expression levels.The expression of BCL-2 and BCL-6 and its association with clinic-pathological features and prognosis were analyzed.Results BCL-2 protein expression was performed in 47 B-NHL,the rate of positivity in BL and DLBCL were 9 .7% (3/3 1 ) and 33 .3%(5/15),respectively.There was significant difference between them (P=0.047).However,there was no association between the expression of BCL-2 and gender,stage and prognosis (P>0.05 ).BCL-6 protein expression was performed in 31 B-NHL,The 2-year EFS was 83.3% for children with BCL-6 protein expression,the other was 45.5%,there was significant difference between them (P=0.019). Children with positive BCL-6 protein expression had better prognosis.Conclusion BCL-2 protein expression was useful for distinguish BL from DLBCL and BCL-6 protein expression is a prognosis factor of B-NHL.