CAJ | 학술논문

目的观察奥沙利铂低剂量节律化疗(LDM)在小鼠胃癌中的抑瘤效果、不良反应并探讨其机制.方法 BALB/c-nu小鼠皮下接种胃癌细胞株MGC-803细胞,随机分为3组,每组20只.节律组:奥沙利铂1 mg/(kg·d)腹腔注入,持续2周;最大耐受剂量(MTD)组:1次腹腔注入奥沙利铂14 mg/(kg·d);对照组:生理盐水0.2 ml每日腹腔注入,持续2周.接种后第15天处死小鼠,完整剥离皮下瘤结节,计算抑瘤率,行组织学观察并采用免疫组织化学法检测肿瘤内微血管密度(MVD)、血管内皮生长因子(VEGF)表达.结果节律组及MTD组抑瘤率分别为59.6％、39.4％,差异有统计学意义(P＜0.05),节律组肿瘤组织可见大量的细胞变性坏死,MTD组少见.节律组肿瘤组织MVD及VEGF蛋白表达较MTD组显著减少(P＜0.01).结论奥沙利铂低剂量节律化疗可显著抑制BALB/c-nu小鼠胃癌肿瘤的生长,其机制可能为奥沙利铂下调VEGF表达,抑制肿瘤血管生成,进而抑制肿瘤生长.
목적 관찰오사리박저제량절률화료(LDM)재소서위암중적억류효과、불량반응병탐토기궤제.방법 BALB/c-nu소서피하접충위암세포주MGC-803세포,수궤분위3조,매조20지.절률조:오사리박1 mg/(kg·d)복강주입,지속2주;최대내수제량(MTD)조:1차복강주입오사리박14 mg/(kg·d);대조조:생리염수0.2 ml매일복강주입,지속2주.접충후제15천처사소서,완정박리피하류결절,계산억류솔,행조직학관찰병채용면역조직화학법검측종류내미혈관밀도(MVD)、혈관내피생장인자(VEGF)표체.결과 절률조급MTD조억류솔분별위59.6％、39.4％,차이유통계학의의(P＜0.05),절률조종류조직가견대량적세포변성배사,MTD조소견.절률조종류조직MVD급VEGF단백표체교MTD조현저감소(P＜0.01).결론 오사리박저제량절률화료가현저억제BALB/c-nu소서위암종류적생장,기궤제가능위오사리박하조VEGF표체,억제종류혈관생성,진이억제종류생장.
Objective To explore the inhibitory effect of low-dose metronomic Oxaliplatin chemotherapy on gastric cancer growth in mice and the action mechansim.Methods Sixty BALB/c-nu mice were subcutaneously implanted with MGC-803 cells,and evenly divided into three groups:Low-dose metronomic (LDM) group,Oxaliplatin 1 mg/(kg· d),once a day for two weeks; Maximum tolerated dose (MTD) group:Oxaliplatin 14 mg/(kg·d) single dose,and intraperitoneal administration; control group:intraperitoneal injection of 1.2 ml normal saline,once every day for 2 weeks.The mice were killed on the day 15.The tumors were then weighed,and tumor microvessel density (MVD) and the expression of vascular endothelial growth factor (VEGF) were detected by immunohistochemical staining.Results The cancer suppressive rate in LDM and MTD groups was 59.6％ and 39.4％ respectively.The expression of VEGF and MVD was significantly lower in LDM group than in MTD group (P ＜ 0.05).Conclusion Angiogenesis is significantly inhibited in mice receiving LDM treatment,which may be correlated with the low wexpression of VEGF in the tumors.