食品与发酵科技
食品與髮酵科技
식품여발효과기
SICHUAN FOOD AND FERMENTATION
2014年
2期
80-84,88
,共6页
实时荧光定量PCR%食源性致病菌%检测
實時熒光定量PCR%食源性緻病菌%檢測
실시형광정량PCR%식원성치병균%검측
real-time fluorescent quantitative PCR%food-borne pathogenic bacteria%detection
近年来食源性疾病爆发数量呈上升趋势，食品安全问题已经成为危害人们生命和健康的严重问题，引起了全世界消费者对食品品质、安全和卫生的关注。实时荧光定量PCR技术是在定性PCR技术的基础上发展起来的核酸定量技术，灵敏性高、特异性强，实现了对DNA模板的定量检测，目前已广泛应用于食品检测领域。本文概述了实时荧光定量PCR技术的原理及其在食源性致病菌检测中的应用，并对其应用前景进行了探讨。
近年來食源性疾病爆髮數量呈上升趨勢，食品安全問題已經成為危害人們生命和健康的嚴重問題，引起瞭全世界消費者對食品品質、安全和衛生的關註。實時熒光定量PCR技術是在定性PCR技術的基礎上髮展起來的覈痠定量技術，靈敏性高、特異性彊，實現瞭對DNA模闆的定量檢測，目前已廣汎應用于食品檢測領域。本文概述瞭實時熒光定量PCR技術的原理及其在食源性緻病菌檢測中的應用，併對其應用前景進行瞭探討。
근년래식원성질병폭발수량정상승추세，식품안전문제이경성위위해인문생명화건강적엄중문제，인기료전세계소비자대식품품질、안전화위생적관주。실시형광정량PCR기술시재정성PCR기술적기출상발전기래적핵산정량기술，령민성고、특이성강，실현료대DNA모판적정량검측，목전이엄범응용우식품검측영역。본문개술료실시형광정량PCR기술적원리급기재식원성치병균검측중적응용，병대기응용전경진행료탐토。
The outbreak of food-borne disease has shown a rising trend in recent years, and the problem of food safety seriously threats people’s life and health. Consumers around the world pay much attention to food quality, safety and health. Real-time fluorescent quantitative PCR technique is a nucleic acid quantitative technique, on the basis of the qualitative PCR technology, having high sensitivity, strong specificity, which has realized the quantita-tive detection of DNA template, and now has been widely used in the field of food detection. This paper summa-rized the principle of real-time fluorescent quantitative PCR technique and its application in the detection of food-borne pathogenic bacteria, and discussed the application prospect.
