CAJ | 학술논문

为获取高效表达且具有抗菌活性的新型杂合抗菌抗菌肽。根据 Spinigerin α、Japonicin II 抗菌肽的氨基酸序列，结合蛋白质分子的结构和抗菌肽的抑菌机制，选用毕赤酵母偏爱密码子，设计合成杂合抗菌肽 Sα-Jp基因，并将其定向克隆至真核表达载体 pPICZαA 上。经聚合酶链式反应，双酶切，序列分析等鉴定，所转化的宿主菌中含有插入 Sα-Jp 基因的重组表达载体 pPICZαA-Sα-Jp。结果表明：已成功构建真核表达载体，可用于真核分泌表达。
위획취고효표체차구유항균활성적신형잡합항균항균태。근거 Spinigerin α、Japonicin II 항균태적안기산서렬，결합단백질분자적결구화항균태적억균궤제，선용필적효모편애밀마자，설계합성잡합항균태 Sα-Jp기인，병장기정향극륭지진핵표체재체 pPICZαA 상。경취합매련식반응，쌍매절，서렬분석등감정，소전화적숙주균중함유삽입 Sα-Jp 기인적중조표체재체 pPICZαA-Sα-Jp。결과표명：이성공구건진핵표체재체，가용우진핵분비표체。
To obtain the new hybrid antibacterial peptide of high efficient expression and antimicrobial activity. Ac-cording to the sequence of amino acids about Spinigerin α, Japonicin II antibacterial peptide, combined with the structure of the protein molecules and antibacterial mechanism of antibacterial peptides , choose pichia preference codon, design and synthesis hybrid antimicrobial peptide Sα-Jp gene, and directed cloning to eukaryotic expression vector pPICZαA. By polymerase chain reaction, the double enzyme digestion and the sequence analysis to identify the transformation of host insert is contained in the recombinant expression vector of pPICZαA-Sα-Jp. Results show that we have been successfully build a eukaryotic expression vector, and it can be used in the secretion of eu-karyotic expression.