CAJ | 학술논문

[目的]利用原子力显微镜对新布尼亚病毒进行观察。[方法]超速离心制备新布尼亚病毒HB29株，采用磷钨酸负染透射电子显微镜进行观察；制备新布尼亚病毒的原子力显微镜样片，在此基础上对病毒进行原子力显微镜观察，采用轻敲模式在大气常温下进行扫描成像。[结果]透射电子显微镜观察到新布尼亚病毒球形病毒粒子，并提供病毒二维图像，可见刺突结构；原子力显微镜则呈现了新布尼亚病毒三维图像，且可见病毒表面有凹凸不平的特征和边缘不平滑，同时获得表面粗糙度等可量化指标。两种方法最终得到相似的形态学结果。[结论]利用原子力显微镜首次观察到新布尼亚病毒的三维形态结构，与透射电镜观察相比，原子力显微镜是一种制样简单，观察直观的新型病毒形态学研究工具。
[목적]이용원자력현미경대신포니아병독진행관찰。[방법]초속리심제비신포니아병독HB29주，채용린오산부염투사전자현미경진행관찰；제비신포니아병독적원자력현미경양편，재차기출상대병독진행원자력현미경관찰，채용경고모식재대기상온하진행소묘성상。[결과]투사전자현미경관찰도신포니아병독구형병독입자，병제공병독이유도상，가견자돌결구；원자력현미경칙정현료신포니아병독삼유도상，차가견병독표면유요철불평적특정화변연불평활，동시획득표면조조도등가양화지표。량충방법최종득도상사적형태학결과。[결론]이용원자력현미경수차관찰도신포니아병독적삼유형태결구，여투사전경관찰상비，원자력현미경시일충제양간단，관찰직관적신형병독형태학연구공구。
The morphology of the severe fever with thrombocytopenia syndrome bunya virus(SFTSV) was investigated by the atomic force microscopy(AFM) . The ultracentrifuged SFTSV HB29 strains were negatively stained by phosphotungstic acid, and observed under the transmission electron microscopy (TEM). The SFTSV sample was prepared according to an AFM protocol, and then the SFTSV image was scanned with tapping mode at room temperature. The two-dimensional TEM image revealed a classical spike like sphere structure. The three-dimensional AFM image showed the characteristics and the morphology of the SFTSV strains. The SFTSV particle was an irregular sphere with an uneven edge. The measurements of the uneven edge were obtained in this study. This is the first time to have got the three-dimensional morphological structure of SFTSV by AFM. AFM is a new research tool for viral morphological observation with the advantages of simple preparing, 3D direct-viewing and visible interfaceing.