CAJ | 학술논문

目的：探讨吗啡对大鼠嗜铬细胞瘤 PC12细胞增殖的抑制作用及其分子机制。方法 PC12细胞体外培养，将吗啡加入细胞培养液中使吗啡浓度分别稀释至10、20、30μmol/L ，然后分别与 PC12细胞一同孵育，四甲基偶氮噻唑蓝法（MTT）观察吗啡对细胞增殖的抑制作用；免疫印迹法检测PC12细胞p38MAPK 表达及磷酸化水平。结果不同浓度吗啡对PC12细胞增殖有明显的抑制作用，抑制率为17．66％～31．05％；并且吗啡可以诱导PC12细胞p38MAPK 磷酸化水平升高，而p38MAPK 通路阻断剂SB203580可以抑制吗啡诱导 PC12细胞 p38MAPK 磷酸化水平升高，减轻吗啡对 PC12细胞增殖的抑制。结论吗啡可以明显抑制 PC12细胞的增殖，升高p38MAPK 的磷酸化水平可能是其重要分子机制之一。
목적：탐토마배대대서기락세포류 PC12세포증식적억제작용급기분자궤제。방법 PC12세포체외배양，장마배가입세포배양액중사마배농도분별희석지10、20、30μmol/L ，연후분별여 PC12세포일동부육，사갑기우담새서람법（MTT）관찰마배대세포증식적억제작용；면역인적법검측PC12세포p38MAPK 표체급린산화수평。결과불동농도마배대PC12세포증식유명현적억제작용，억제솔위17．66％～31．05％；병차마배가이유도PC12세포p38MAPK 린산화수평승고，이p38MAPK 통로조단제SB203580가이억제마배유도 PC12세포 p38MAPK 린산화수평승고，감경마배대 PC12세포증식적억제。결론마배가이명현억제 PC12세포적증식，승고p38MAPK 적린산화수평가능시기중요분자궤제지일。
Objective To investigate the effects of morphine on roliferation of pheochromocytoma cell and its mechanism. Methods PC12 cell cultures were exposed to 10,20,30 μmol/L morphine. The inhibition of the cell proliferation was determined by MTT assay. The level of p38MAPK was detected by immunoblotting. Results The proliferation of PC12 cells were inhibited after morphine exposure for 48 h.Immunoblotting showed that morphine increased the phosphorylation of p38MAPK was increased in stimulation of monphine. SB203580 , the inhibitor of p38MAPK , reduced inhibition the proliferation of PC12 cells by morphine. Conclusion These results suggest that morphine can significantly inhibits the proliferation of PC12 cells by an increase in p38MAPK phosphorylation.