CAJ | 학술논문

目的：通过建立人免疫重建重症联合免疫缺陷（SCID）荷瘤鼠嵌合模型来探讨4-1BBL-B7-H3基因在非特异性抗肿瘤免疫中的作用。方法将40只SCID鼠随机分为5组，A组（对照组）行免疫重建加注射Tca8113细胞；B组（Ad4-1BBL-B7-H3组）行免疫重建加注射含有人4-1BBL-B7-H3基因腺病毒转染的Tca8113细胞；C组（空载组）行免疫重建加注射含有空载体腺病毒转染的Tca8113细胞；D组（非免疫重建组）不给予小鼠免疫重建，注射Tca8113细胞；E组（非肿瘤组）行免疫重建加注射PBS。每周定期测量肿瘤体积；酶联免疫吸附法检测人IgG蛋白含量；流式细胞仪检测外周血中人CD3+、CD56+淋巴细胞比例；免疫组织化学法观察自然杀伤细胞2族成员D（NKG2D）和Toll样受体2（TLR2）在肿瘤中的表达；逆转录聚合酶链反应（RT-PCR）检测4-1BBL-B7-H3mRNA的表达，实时定量聚合酶链反应（RT-qPCR）检测主要组织相容性复合体1类相关分子（M1C）A、B及TLR2的表达。结果1）B组小鼠肿瘤体积最小（P<0.05）；2）A、B、C、E组小鼠外周血中检测到人IgG、CD3+、CD56+淋巴细胞，B组淋巴细胞比例高于A、C和E组（P<0.05）；3）B组NKG2D和TLR2的表达较其余各组明显增强；4）人4-1BBL-B7-H3基因在B组小鼠肿瘤中稳定表达；5）B组M1CA、M1CB和TLR2的表达高于A、C、D组（P<0.05）。结论4-1BBL-B7-H3基因在肿瘤组织中的高表达能成功诱导人CD3+、CD56+细胞增殖，直接或间接活化TLR2，上调NKG2D及其配体M1CA、M1CB的表达，从而产生有效的抗肿瘤免疫应答。
목적：통과건립인면역중건중증연합면역결함（SCID）하류서감합모형래탐토4-1BBL-B7-H3기인재비특이성항종류면역중적작용。방법장40지SCID서수궤분위5조，A조（대조조）행면역중건가주사Tca8113세포；B조（Ad4-1BBL-B7-H3조）행면역중건가주사함유인4-1BBL-B7-H3기인선병독전염적Tca8113세포；C조（공재조）행면역중건가주사함유공재체선병독전염적Tca8113세포；D조（비면역중건조）불급여소서면역중건，주사Tca8113세포；E조（비종류조）행면역중건가주사PBS。매주정기측량종류체적；매련면역흡부법검측인IgG단백함량；류식세포의검측외주혈중인CD3+、CD56+림파세포비례；면역조직화학법관찰자연살상세포2족성원D（NKG2D）화Toll양수체2（TLR2）재종류중적표체；역전록취합매련반응（RT-PCR）검측4-1BBL-B7-H3mRNA적표체，실시정량취합매련반응（RT-qPCR）검측주요조직상용성복합체1류상관분자（M1C）A、B급TLR2적표체。결과1）B조소서종류체적최소（P<0.05）；2）A、B、C、E조소서외주혈중검측도인IgG、CD3+、CD56+림파세포，B조림파세포비례고우A、C화E조（P<0.05）；3）B조NKG2D화TLR2적표체교기여각조명현증강；4）인4-1BBL-B7-H3기인재B조소서종류중은정표체；5）B조M1CA、M1CB화TLR2적표체고우A、C、D조（P<0.05）。결론4-1BBL-B7-H3기인재종류조직중적고표체능성공유도인CD3+、CD56+세포증식，직접혹간접활화TLR2，상조NKG2D급기배체M1CA、M1CB적표체，종이산생유효적항종류면역응답。
Objective The non-specific antitumor immunity effect of 4-1BBL-B7-H3 gene was investigated by establishing an oral squamous cell carcinoma human peripheral blood lymphocyte-severe combined immunodeficient (SCID) mice chimeric model. Methods Forty mice were randomly divided into five groups. All groups, except the non-immune reconstitution group (group D), had reconstructed human partial immune system. The control group (group A) was injected with Tca8113 cells. The Ad4-1BBL-B7-H3 group (group B) was injected with Tca8113 cells transfected by adenovirus containing 4-1BBL-B7-H3 gene. The empty vector group (group C) was injected with Tca8113 cells transfected by adenovirus containing an empty vector. The non-immune reconstitution group (group D) was injected with Tca8113 cells. The non-tumor group (group E) was injected with PBS. The tumor volumes in each group were measured weekly. Human IgG in blood was obtained through the tail vein and was determined by enzyme-linked immu-nosorbent assay. Human CD3+ and D56+ lymphocytes were assessed by flow cytometry. Model animals were killed on the ninth week. Differences in the expression of the natural killer group 2 member D (NKG2D) and toll-like receptor 2 (TLR2) in tumor tissues of each group were observed by im-munohistochemical method. 4-1BBL-B7-H3 gene expression in mice tumor tissues was detected by reverse transcription polymerase chain reaction (PCR) and the expressions of major histocompatibility complex 1 class related molecule (M1C) A, M1CB, and TLR2 were detected by real-time quantitative PCR. Results The tumor volumes of group B were remarkably lower than those in the other groups (P<0.05). Human IgG and CD3+ and CD56+ lymphocytes were detected in the peripheral blood of immune-reconstituted mice. These lymphocytes were remarkably higher in group B than those in groups A, C, and E (P<0.05). Higher NKG2D and TLR2 expression were observed in group B tumor than those in the other groups. The stable expression of 4-1BBL-B7-H3 gene in group B was proven. The expression of M1CA, M1CB, and TLR2 were significantly higher in the group B tumor than those in groups A, C, and D (P<0.05). Conclusion The high 4-1BBL-B7-H3 gene expression in tumor tissues could successfully induce the proliferation of CD3+ and CD56+ lymphocytes. This expression can also directly or indirectly activate TLR2 and up-regulate the expression of NKG2D and its ligands (M1CA and M1CB), which result in an effective antitumor immune response.