CAJ | 학술논문

目的：通过聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析双生子儿童中同卵双生子和异卵双生子口腔微生物群组结构的差异。方法选取双生子儿童20对，其中同卵双生子10对，异卵双生子10对；乳牙列10对，混合牙列10对；有龋者17人，无龋者23人。采集唾液样本后，提取细菌DNA，经通用引物扩增16srRNA基因和PCR-DGGE分析后，计算PCR-DGGE条带数及多样性指数。结果同卵双生子与异卵双生子均表现出明显的聚类分布，但同卵双生子与异卵双生子之间无统计学差异（P>0.05）。乳牙列组中，有龋儿童的PCR-DGGE条带数（11.00±1.56）及多样性指数（1.05±0.36）低于无龋儿童（14.00±2.74，1.44±0.37），两者间均具有统计学差异（P<0.05）。混合牙列组中，有龋儿童的PCR-DGGE条带数（11.88±4.05）及多样性指数（1.18±0.36）低于无龋儿童（14.31±5.71，1.28±0.47），但两者间均无统计学差异（P>0.05）。结论亲缘之间口腔微生物组群结构更为相似，环境的影响可能大于遗传的影响。有龋儿童的微生物种类较无龋儿童有所减少。
목적：통과취합매련반응-변성제도응효전영（PCR-DGGE）분석쌍생자인동중동란쌍생자화이란쌍생자구강미생물군조결구적차이。방법선취쌍생자인동20대，기중동란쌍생자10대，이란쌍생자10대；유아렬10대，혼합아렬10대；유우자17인，무우자23인。채집타액양본후，제취세균DNA，경통용인물확증16srRNA기인화PCR-DGGE분석후，계산PCR-DGGE조대수급다양성지수。결과동란쌍생자여이란쌍생자균표현출명현적취류분포，단동란쌍생자여이란쌍생자지간무통계학차이（P>0.05）。유아렬조중，유우인동적PCR-DGGE조대수（11.00±1.56）급다양성지수（1.05±0.36）저우무우인동（14.00±2.74，1.44±0.37），량자간균구유통계학차이（P<0.05）。혼합아렬조중，유우인동적PCR-DGGE조대수（11.88±4.05）급다양성지수（1.18±0.36）저우무우인동（14.31±5.71，1.28±0.47），단량자간균무통계학차이（P>0.05）。결론친연지간구강미생물조군결구경위상사，배경적영향가능대우유전적영향。유우인동적미생물충류교무우인동유소감소。
Objective To analyze the differences between the oral microbiota of monozygotic and dizygotic twins by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Methods A total of 20 pairs of twin children were included in this study, in which 10 pairs were monozygotic (MZ) twins, and 10 pairs were dizygotic (DZ) twins. Of the 20 pairs, 10 pairs of twins had primary dentition, and 10 pairs had mixed dentition; 17 children had caries, and 23 children had no caries. Genomic DNA was extracted from saliva samples. The 16s rRNA was amplified and analyzed by PCR-DGGE. The PCR-DGGE band number and Shannon index were calculated. Results Cluster analysis showed high similarity in the oral bacterial community seen in co-twins. However, no significant difference was seen between MZ and DZ twins. In the primary dentition, the PCR-DGGE band number and Shannon index of children with caries (11.00±1.56, 1.05±0.36) were lower than those of children without caries (14.00±2.74, 1.44±0.37) (P<0.05). In mixed dentition, the PCR-DGGE band number and Shannon index of children with caries (11.88±4.05, 1.18±0.36) were lower than those of children without caries (14.31± 5.71, 1.28±0.47), but the differences were not statistically significant (P>0.05). Conclusion Environmental factors may have a stronger effect on the constitution of oral microbiota in children compared with genetic factors. Children without caries may have a richer microbial diversity compared with children with caries.