CAJ | 학술논문

目的：初步探讨健康人群颊黏膜菌群组成在不同年龄及牙列阶段的动态演替过程。方法25例健康受试者根据年龄及牙列情况分为5组：乳牙列组、混合牙列组、青少年组、青年组及老年组；分别提取各样本的细菌总DNA进行聚合酶链式反应，采用QuantityOne软件对扩增产物的变性梯度凝胶电泳（DGGE）指纹图谱进行生物信息学分析。结果1）颊黏膜菌群的组成具有个体差异性。2）乳牙列组、混合牙列组、青少年组、青年组及老年组DGGE指纹图谱的平均条带数分别为21.2±4.0、17.8±3.9、15.8±4.3、16.8±3.7、22.2±6.5，各组间的差异无统计学意义（P>0.05），提示颊黏膜优势菌群的数量在各个年龄阶段保持相对稳定。3）5组的Shannon指数分别为1.73±0.2、1.43±0.1、1.05±0.2、1.45±0.2和1.63±0.3，各组间的差异有统计学意义（P=0.003）；提示颊黏膜微生物的多样性在儿童至青少年阶段呈下降趋势，在青年至老年阶段呈上升趋势。4）群落结构相似性聚类分析发现：同组内大部分样本聚类位置相近，群落结构表现出较高的相似性；不同组的大部分样本未聚类在一起，群落结构呈现出一定的差异性。结论不同年龄组健康人群的颊黏膜微生物群落组成存在差异，青少年期可能是颊黏膜微生物多样性改变的重要转折点。
목적：초보탐토건강인군협점막균군조성재불동년령급아렬계단적동태연체과정。방법25례건강수시자근거년령급아렬정황분위5조：유아렬조、혼합아렬조、청소년조、청년조급노년조；분별제취각양본적세균총DNA진행취합매련식반응，채용QuantityOne연건대확증산물적변성제도응효전영（DGGE）지문도보진행생물신식학분석。결과1）협점막균군적조성구유개체차이성。2）유아렬조、혼합아렬조、청소년조、청년조급노년조DGGE지문도보적평균조대수분별위21.2±4.0、17.8±3.9、15.8±4.3、16.8±3.7、22.2±6.5，각조간적차이무통계학의의（P>0.05），제시협점막우세균군적수량재각개년령계단보지상대은정。3）5조적Shannon지수분별위1.73±0.2、1.43±0.1、1.05±0.2、1.45±0.2화1.63±0.3，각조간적차이유통계학의의（P=0.003）；제시협점막미생물적다양성재인동지청소년계단정하강추세，재청년지노년계단정상승추세。4）군락결구상사성취류분석발현：동조내대부분양본취류위치상근，군락결구표현출교고적상사성；불동조적대부분양본미취류재일기，군락결구정현출일정적차이성。결론불동년령조건강인군적협점막미생물군락조성존재차이，청소년기가능시협점막미생물다양성개변적중요전절점。
Objective This investigation aimed to examine how buccal mucosa microbiome succeeds in a healthy population with different ages and dentition stages. Methods Twenty-five subjects were recruited and subdivided into five groups: pri-mary dentition group, mixed dentition group, adolescent group, adult group, and elderly group. Individual mucosal microbiota was obtained by gently scraping both sides of the buccal mucosa with a cotton swab. Microbial diversity was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Results 1) The composition of buccal mucosa microbiota has great intra-individual divergence. 2) The average band numbers of the primary dentition group, mixed dentition group, adolescent group, adult group, and elderly group were 21.2±4.0, 17.8±3.9, 15.8±4.3, 16.8±3.7, and 22.2±6.5, respectively. No between-group differences was observed (P>0.05), indicating that predominant strains in the oral cavity may be stable throughout an individual’s lifetime. 3) The Shannon indices of primary dentition group, mixed dentition group, adolescent group, adult group, and elderly group were 1.73±0.2, 1.43±0.1, 1.05±0.2, 1.45±0.2, and 1.63±0.3, respectively. A significant between-group difference was observed (P=0.003), indicating that the microbial diversity of the buccal mucosa decreases from childhood through adolescence, but increases from adult through senescence. 4) The clustering analysis showed that most of the samples in the same group clustered together, indicating higher intra-group community structure similarity. Conclusion Composition of the buccal mucosa microbiota was different among age groups. Adolescence may be an essential turning point of microbial ecology succession throughout life.