CAJ | 학술논문

目的：探讨CC族趋化因子受体9（ CCR9）在非小细胞肺癌（ NSCLC ）肿瘤免疫机制中的作用。方法采用流式细胞术检测42例NSCLC患者和30名健康人手术前后T细胞亚群以及外周血中CD4＋T淋巴细胞表面CCR9的表达情况，计数各组细胞表达的百分率。免疫磁珠分选外周血CD4＋T淋巴细胞，采用transwell实验检测并分析CCL25/CCR9对CD4＋T淋巴细胞迁移的影响。结果 NSCLC患者外周血T淋巴细胞亚群均降低，术后外周血CD4＋T淋巴细胞、CD4＋/CD8＋比值均明显高于术前[（49.11±8.32） vs （46.17±8.71），P＝0.031和（1.66±0.09） vs （1.44±0.06），P＝0.001]；术前CD4＋CCR9＋T淋巴细胞的百分率低于术后[（3.33±1.11） vs （6.57±1.92），P＜0.05]和健康对照组[（3.33±1.11） vs （11.06±1.37），P＜0.05]。在CCL25诱导下，NSCLC患者外周血CD4＋T淋巴细胞趋化指数（CI）为3.14，明显低于健康对照组的3.83（ P＜0.05）。经过anti-CCR9单抗处理后，CD4＋T淋巴细胞的CI为0.62，与未经anti-CCR9 mAb处理者相比明显降低（ P＜0.05）。结论 NSCLC患者外周血T淋巴细胞调节机制紊乱，CL25/CCR9相互作用可介导外周血CD4＋T淋巴细胞迁移，NSCLC患者外周血淋巴细胞中CCR9低表达，影响淋巴细胞迁移，可能与肿瘤逃避免疫监视的机制有关。手术可以逆转CD4＋T淋巴细胞表面CCR9的表达变化， CCR9可能作为评价肺癌治疗后免疫重建的指标。
목적：탐토CC족추화인자수체9（ CCR9）재비소세포폐암（ NSCLC ）종류면역궤제중적작용。방법채용류식세포술검측42례NSCLC환자화30명건강인수술전후T세포아군이급외주혈중CD4＋T림파세포표면CCR9적표체정황，계수각조세포표체적백분솔。면역자주분선외주혈CD4＋T림파세포，채용transwell실험검측병분석CCL25/CCR9대CD4＋T림파세포천이적영향。결과 NSCLC환자외주혈T림파세포아군균강저，술후외주혈CD4＋T림파세포、CD4＋/CD8＋비치균명현고우술전[（49.11±8.32） vs （46.17±8.71），P＝0.031화（1.66±0.09） vs （1.44±0.06），P＝0.001]；술전CD4＋CCR9＋T림파세포적백분솔저우술후[（3.33±1.11） vs （6.57±1.92），P＜0.05]화건강대조조[（3.33±1.11） vs （11.06±1.37），P＜0.05]。재CCL25유도하，NSCLC환자외주혈CD4＋T림파세포추화지수（CI）위3.14，명현저우건강대조조적3.83（ P＜0.05）。경과anti-CCR9단항처리후，CD4＋T림파세포적CI위0.62，여미경anti-CCR9 mAb처리자상비명현강저（ P＜0.05）。결론 NSCLC환자외주혈T림파세포조절궤제문란，CL25/CCR9상호작용가개도외주혈CD4＋T림파세포천이，NSCLC환자외주혈림파세포중CCR9저표체，영향림파세포천이，가능여종류도피면역감시적궤제유관。수술가이역전CD4＋T림파세포표면CCR9적표체변화， CCR9가능작위평개폐암치료후면역중건적지표。
Objective To investigate the expression of CC chemokine receptor 9 ( CCR9 ) on peripheral blood CD4 +T lymphocyte cells of non small cell lung cancer ( NSCLC), and the effect of CCR9 and its ligand (CCL25) on peripheral blood CD4+T lymphocytes.Methods The study was performed in 42 NSCLC patients and 30 healthy controls .Flow cytometry was employed to detect the T lymphocyte subsets and the expression of CCR 9 on peripheral blood CD4 +T cells.Peripheral blood CD4 +T lymphocytes were isolated from all cases with magnetic-acti-vated cell sorting method .The cell trans-membrane test with 24-transwell was used to detect the effect of CCR 9/CCL25 on the lymphocyte migration .Results Compared with control group , the T lymphocyte subsets in pre-opera-tive patients were higher than that in post-operative patients(P<0.05).The frequency of CD4 +CCR9+T lympho-cyte in pre-operative group was both significantly decreased compared with post-operative group(3.33 ±1.11 vs 6.57 ± 1.92,P<0.05) and controls(3.33 ±1.11 vs 11.06 ±1.37,P<0.05).In the induce of CCL25, the chemotactic indexes(CI) of CD4 +T lymphocytes from NSCLC patients (3.14) was significantly lower than that from healthy per-sons(3.83).After anti-CCR9 mAb pretreatment, the lymphocytes CIs of NSCLC patients decreased significantly to 0.62 as compared with untreated controls .Conclusion The expression of CCR9 is down-regulated on NSCLC periph-eral blood CD4+T lymphocyte cells .CCR9/CCL25 interactions mediate the migration of CD 4 +T lymphocytes in bodies .The decreased expression of CCR 9 in the CD4+T lymphocytes of patients with NSCLC could effect the ability of lymphocytes migration , which might involve in the mechanisms of tumor escape immune surveillance .Operation could reverse the change of CCR 9 expression on CD4 +T lymphocyte cells .CCR9 may be suggested to indicate immu-nologic reconstitution after therapy and operation .