CAJ | 학술논문

目的：探讨Caveolin-1对下肢缺血糖尿病大鼠缺血部位血管形成的作用及与AKT信号通路的关系。方法健康、雄性SD大鼠，在腹腔注射链脲菌素构建的1型糖尿病大鼠模型上离断左侧股动脉及其分支，建立急性下肢缺血动物模型，14 d后用Western blot检测Caveolin-1、AKT的表达水平；28 d后进行CD34免疫组织化学染色和HE染色，评价血管密度。结果缺血组和糖尿病组与假手术组相比，Caveolin-1蛋白的表达水平明显增加(P<0.05)；且缺血组Caveolin-1蛋白含量显著高于糖尿病组(P<0.05)。缺血组、缺血转染组、缺血空转染组的Caveolin-1蛋白、AKT水平均明显高于假手术组(P<0.05)；缺血转染组与缺血组相比，Caveolin-1蛋白、AKT的表达水平明显增多(P<0.05)。与假手术组相比，缺血组、缺血转染组和缺血空转染组的微血管均有一定程度增加，其中缺血转染组的微血管密度增加尤为显著(P<0.05)。结论 Caveolin-1过表达后对糖尿病大鼠下肢缺血部位的血管形成有明显的促进作用，可能与激活AKT有关。
목적：탐토Caveolin-1대하지결혈당뇨병대서결혈부위혈관형성적작용급여AKT신호통로적관계。방법건강、웅성SD대서，재복강주사련뇨균소구건적1형당뇨병대서모형상리단좌측고동맥급기분지，건립급성하지결혈동물모형，14 d후용Western blot검측Caveolin-1、AKT적표체수평；28 d후진행CD34면역조직화학염색화HE염색，평개혈관밀도。결과결혈조화당뇨병조여가수술조상비，Caveolin-1단백적표체수평명현증가(P<0.05)；차결혈조Caveolin-1단백함량현저고우당뇨병조(P<0.05)。결혈조、결혈전염조、결혈공전염조적Caveolin-1단백、AKT수평균명현고우가수술조(P<0.05)；결혈전염조여결혈조상비，Caveolin-1단백、AKT적표체수평명현증다(P<0.05)。여가수술조상비，결혈조、결혈전염조화결혈공전염조적미혈관균유일정정도증가，기중결혈전염조적미혈관밀도증가우위현저(P<0.05)。결론 Caveolin-1과표체후대당뇨병대서하지결혈부위적혈관형성유명현적촉진작용，가능여격활AKT유관。
Objective To investigate the function of Caveolin-1 on angiogenesis in lower limb ischemia region of dia-betic rat and the relationship with AKT signal pathway. Methods Intraperitoneal injection of healthy,male,SD rats with streptozocin to construct type 1 diabetic rat model,then broken the left femoral artery to set up the acute lower limb is-chemia animal model.After 14 days with Western blot detection Caveolin-1 and AKT expression.CD34 immunohisto-chemical staining and HE staining was applied to detect the vascular density in 28 days. Results The level of Cave-olin-1 in diabetes group and ischemia group was obviously higher than that in sham group respectively,the level of Caveolin-1 in ischemia group was higher than that in diabetes group,with statistical difference (P<0.05).The level of Caveolin-1 and AKT in ischemia group,ischemic transfection group,ischemic idling transfected group was obviously higher than that in sham group respectively,the level of Caveolin-1 and AKT in ischemic transfection group was obvi-ously higher than that in ischemia group respectively,with statistical difference(P<0.05).Compared with the sham opera-tion group,microvascular had a certain degree increased in ischemia group,ischemia transfection group and ischemic idling transfected group,the microvessel density in the ischemic transfection group increased significantly (P<0.05). Conclusion The higher expression of Caveolin-1 in diabetic lower limb ischemia in rats has obvious promoting effect to its angiogenesis,this effect may be related to activation of AKT.